Western blot was performed using Calreticulin Polyclonal Antibody (Product # PA1-16924) and a ~55kDa band corresponding to Calreticulin was observed across all the cell lines tested with a uncharacterized band (*). Whole cell extracts (30 µg lysate) of HL-60 (Lane 1), LNCaP(Lane 2), HeLa (Lane 3), MCF-7 (Lane 4), Mouse Liver (Lane 5) and Rat Liver (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).