IFN-β (7F-D3) Antibody sc-57201 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemIFN-β (7F-D3) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-57201
Antibody TypePrimary Antibodies
Clone7F-D3
Quantity100 µg/ml
ApplicationsWB, IP, ELISA
Reactivitymouse
Conjugate/TagUnconjugated
Hostrat
FormatPurified
ClonalityMonoclonal
TargetIFN-beta
IsotypeIgG1
NCBI Full Gene Nameinterferon beta 1, fibroblast
NCBI Gene AliasesIFN-beta, IFNB, If1da1, Ifb

Questions and Answers

Can this antibody be used for measuring IFN-β levels in mouse samples?

Yes, the IFN-β (7F-D3) antibody has been utilized in ELISA assays for measuring mouse IFN-β levels. It has been shown to effectively capture IFN-β in various studies, including those that detail its application in quantifying cytokine levels in mouse supernatants (, , ).

What is the reactivity of this antibody, and in what species has it been tested?

The IFN-β (7F-D3) antibody is reactive with mouse samples. It has been specifically cited in studies involving murine models, demonstrating its utility in research focused on mouse immune responses (, ).

Has this antibody been referenced in studies related to viral infections?

Yes, the IFN-β (7F-D3) antibody has been referenced in studies investigating viral infections, such as those involving murine norovirus and other RNA viruses. It has been used to assess the type I interferon response during these infections, highlighting its relevance in virology research (, ).

Is there any information on the performance of this antibody in immunoprecipitation assays?

The IFN-β (7F-D3) antibody has been cited for use in immunoprecipitation assays, indicating its effectiveness in isolating IFN-β from biological samples. This application supports its role in studying the dynamics of interferon signaling pathways ().

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Elongator is required for pattern recognition receptor and type I interferon signaling in macrophagesJournal of Biological ChemistryDecember 1, 2025Jamie Murphy, Marcin Baran, Corinna Grünke, Darya Haas, Gillian Barber, Andreas Pichlmair, Andrew G. Bowiesecretion ELISA plates were coated with a monoclonal rat anti-mouse IFNβ capture antibody (Santa Cruz, Cat#SC-57201) in carbonate buffer overnight at 4 °C. Plates were then blocked in blocking solution (10% FCS/PBS)

(2) RNA N-glycosylation enables immune evasion and homeostatic efferocytosisNatureSeptember 1, 2025Graziano VR, Porat J, Ah Kioon MD, Mejdrová I, Matz AJ, Lebedenko CG, Chai P, Pluvinage JV, Ricci-Azevedo R, Harrison AG, et al.mode). ELISA Mouse IFNβ ELISA was performed as described previously49 using an anti-IFNβ monoclonal antibody (sc-57201, Santa Cruz Biotechnology) as a capture antibody and a rabbit polyclonal anti-mouse IFNβ antibody (32400–1,

(3) Delivering LINE1 antisense oligonucleotides via endothelial targeting extracellular vesicles to ameliorate myocardial infarction-induced cardiac senescenceBioact MaterJuly 8, 2025Enze Fu, Kai Pan, Benjamin Hinnant, Shang Chen, Zhibo Han, Zhikun Guo, Zhong-Chao Han, Qiong Li, Zongjin LiCell Signaling Technology), phospho-IRF3 (1:1000; 4947, Cell Signaling Technology), IFN-β (1:1000; sc-57201, Santa Cruz Biotechnology), p53 (1:2000; 2524, Cell Signaling Technology), p21 (1:1000; ab188224, Abcam),

(4) CD14 is a decision-maker between Fas-mediated death and inflammationCell reportsSeptember 24, 2024Magri Z, Jetton D, Muendlein HI, Connolly WM, Russell H, Smirnova I, Sharma S, Bunnell S, Poltorak A.were coated overnight at 4°C with monoclonal rat anti-mouse IFN-β antibody (Santa Cruz Biotechnology, sc-57201; 1:500 dilution in 0.1 M carbonate buffer) and blocked with 10% FBS in PBS for 2 h at 37°C. Samples

(5) Oligoadenylate synthetase 1 displays dual antiviral mechanisms in driving translational shutdown and protecting interferon productionImmunityMarch 1, 2024Munesh K. Harioudh, Joseph Perez, Zhenlu Chong, Sharmila Nair, Lomon So, Kevin D. McCormick, Arundhati Ghosh, Lulu Shao, Rashmi Srivastava, Frank Soveg, et al.neutralization),Leinco Technology,Cat# I-1182 Rat monoclonal anti-IFNβ (ELISA Capture Ab),Santa Cruz Biotechnology,Cat# sc-57201 Rabbit polyclonal anti-IFNβ (ELISA Detection Ab),R&D Systems,Cat# 32400-1 Bacteria and Virus Strains,Bacteria

(6) Neuroimmune characterization of optineurin insufficiency mouse model during ageingScientific reportsJuly 22, 2023Mohovic N, Peradinovic J, Markovinovic A, Cimbro R, Minic Z, Dominovic M, Jakovac H, Nimac J, Rogelj B, Munitic I.(#88-7324-88) were purchased from Invitrogen (Carlsbad, CA, USA). The capture antibody for IFN-β ELISA (#sc-57201) was from Santa Cruz (Dallas, TX, USA), the detection antibody (#32400-1) was from PBL (Piscataway,

(7) PYHIN protein IFI207 regulates cytokine transcription and IRF7 and contributes to the establishment of K. pneumoniae infectionCell reportsApril 25, 2023Baran M, Feriotti C, McGinley A, Carlile SR, Jiang Z, Calderon-Gonzalez R, Dumigan A, Sá-Pessoa J, Sutton CE, Kearney J, et al.

(8) A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3Nature communicationsApril 11, 2023Ta A, Ricci-Azevedo R, Vasudevan SO, Wright SS, Kumari P, Havira MS, Surendran Nair M, Rathinam VA, Vanaja SK.

(9) Neutrophils and macrophages drive TNF-induced lethality via TRIF/CD14-mediated responsesScience immunologyDecember 23, 2022Muendlein HI, Connolly WM, Cameron J, Jetton D, Magri Z, Smirnova I, Vannier E, Li X, Martinot AJ, Batorsky R, et al.ELISA plates were coated overnight at 4°C with monoclonal rat anti-mouse IFNβ antibody (Santa Cruz sc-57201, 1:500 dilution in 0.1M carbonate buffer) and blocked with 10% FBS in PBS for 2hrs at 37°C. Samples

(10) Mitochondrial ROS promotes susceptibility to infection via gasdermin D-mediated necroptosisCellAugust 18, 2022Weindel CG, Martinez EL, Zhao X, Mabry CJ, Bell SL, Vail KJ, Coleman AK, VanPortfliet JJ, Zhao B, Wagner AR, et al.(Cell Signaling Technology #95702), and α-IL-1β (Cell Signaling Technology #12507) α-IFNβ (Santa Cruz Biotechnology # sc-57201) GSDMD/CASP11 protein expression and purification The cDNAs encoding human and mouse GSDMD were inserted

(11) ZBP1 promotes inflammatory responses downstream of TLR3/TLR4 via timely delivery of RIPK1 to TRIFProceedings of the National Academy of Sciences of the United States of AmericaJune 14, 2022Muendlein HI, Connolly WM, Magri Z, Jetton D, Smirnova I, Degterev A, Balachandran S, Poltorak A.384-well ELISA plates coated overnight at 4 °C with monoclonal rat anti-mouse IFNβ antibody (Santa Cruz sc-57201, 1:500 dilution in 0.1 M carbonate buffer) and blocked with 10% FBS in PBS for 2 h at 37 °C. Supernatants

(12) Inhibition of IRGM establishes a robust antiviral immune state to restrict pathogenic virusesEMBO reportsNovember 4, 2021Nath P, Chauhan NR, Jena KK, Datey A, Kumar ND, Mehto S, De S, Nayak TK, Priyadarsini S, Rout K, et al.

(13) Dendritic Cell-Specific Role for Pellino2 as a Mediator of TLR9 Signaling PathwayJournal of immunology (Baltimore, Md. : 1950)November 1, 2021Oleszycka E, Rodgers AM, Xu L, Moynagh PN.

(14) The opportunistic intracellular bacterial pathogen Rhodococcus equi elicits type I interferon by engaging cytosolic DNA sensing in macrophagesPLoS pathogensSeptember 1, 2021Vail KJ, da Silveira BP, Bell SL, Cohen ND, Bordin AI, Patrick KL, Watson RO.Nunc MaxiSorp 96-well plates were coated with 50μl of capture antibody diluted 1:5000 (Santa Cruz, sc-57201) in 0.1M carbonate buffer and were incubated at 4°C overnight. Wells were then blocked using PBS+10%

(15) Murine norovirus virulence factor 1 (VF1) protein contributes to viral fitness during persistent infectionThe Journal of general virologySeptember 1, 2021Borg C, Jahun AS, Thorne L, Sorgeloos F, Bailey D, Goodfellow IG.IFN-β ELISA protocol has been previously described [27]. A monoclonal rat anti-mouse IFN-β antibody (sc57201; Santa Cruz) was used for capture, a polyclonal rabbit anti-mouse IFN-β antibody (32401-1; R and D Systems)

(16) Chitin-derived polymer deacetylation regulates mitochondrial reactive oxygen species dependent cGAS-STING and NLRP3 inflammasome activationBiomaterialsAugust 1, 2021Turley JL, Moran HBT, McEntee CP, O''Grady K, Muñoz-Wolf N, Jin L, Follmann F, Andersen P, Andersson M, Lavelle EC.

(17) AIM2 controls microglial inflammation to prevent experimental autoimmune encephalomyelitisThe Journal of experimental medicineMay 3, 2021Ma C, Li S, Hu Y, Ma Y, Wu Y, Wu C, Liu X, Wang B, Hu G, Zhou J, et al.CCL2 (DY479) according to the manufacturer’s instructions (R&D Systems). IFN-β ELISA capture antibody (sc-57201) was from Santa Cruz; IFN-detection antibody (32400–1) and standard protein (12400–1) were from R&D Systems

(18) STING Agonist Mitigates Experimental Autoimmune Encephalomyelitis by Stimulating Type I IFN-Dependent and -Independent Immune-Regulatory PathwaysJournal of immunology (Baltimore, Md. : 1950)May 1, 2021Johnson BM, Uchimura T, Gallovic MD, Thamilarasan M, Chou WC, Gibson SA, Deng M, Tam JW, Batty CJ, Williams J, et al.protocols. Murine IFN-β ELISA was developed in-house using primary Abs from Santa Cruz Biotechnology (sc-57201) and R&D Systems (32400–1; Minneapolis, MN), rIFN-β standard from R&D Systems (12401–1), and secondary

(19) Hierarchical cell-type-specific functions of caspase-11 in LPS shock and antibacterial host defenseCell reportsApril 20, 2021Kumari P, Russo AJ, Wright SS, Muthupalani S, Rathinam VA.RRID:AB_592012 Rat monoclonal anti-IFN-β antibody (7F-D3; ELISA capture antibody),Santa Cruz Biotechnology,Cat# sc-57201; RRID:AB_2122911 Rabbit polyclonal anti-IFN-β antibody (ELISA detection antibody),PBL,Cat# 32400-1;

(20) TRIM14 Is a Key Regulator of the Type I IFN Response during Mycobacterium tuberculosis InfectionJournal of immunology (Baltimore, Md. : 1950)July 1, 2020Hoffpauir CT, Bell SL, West KO, Jing T, Wagner AR, Torres-Odio S, Cox JS, West AP, Li P, Patrick KL, et al.Nanopore Maxisorp 96-well plates were coated with 50 μl of capture antibody diluted 1:500 (Santa Cruz, #sc-57201) in 0.1M carbonate buffer and were incubate 4°C overnight. Samples were then blocked using PBS+10%FBS

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IFN-β (7F-D3) Antibody

IFN-β (7F-D3) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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IFN-β (7F-D3) Antibody from Santa Cruz Biotechnology, Inc.

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