Cre-Recombinase Antibody 257 003 from Synaptic Systems GmbH

Product Specs
ItemCre-Recombinase Antibody
CompanySynaptic Systems GmbH
Price $385.00Supplier Page View Company Product Page
Catalog Number257 003
Quantity50 µg
ApplicationsICC, IHC, IHC-P
TargetCre-Recombinase
Hostrabbit
Clonalitypolyclonal
Purityaffinity purified
ImmunogenRecombinant protein corresponding to AA 1 to 343 from Bacteriophage P1 Cre-Recombinase

Questions and Answers

Can this Cre-Recombinase antibody be used for immunohistochemistry in brain tissue?

Yes, the Cre-Recombinase antibody has been shown to be effective for immunohistochemistry applications, as evidenced by its use in studies involving brain tissue, including the analysis of serotonergic circuits in the dorsal raphe and the investigation of cerebellar nuclei neurons .

What are the implications of using this antibody in studies involving Cre expression in the central nervous system?

The use of this antibody in studies has highlighted potential toxicity associated with Cre expression in the central nervous system, as demonstrated in research that observed motor deficits and neuroinflammation when Cre was expressed throughout the CNS .

Is this antibody suitable for Western blotting applications?

Yes, the Cre-Recombinase antibody has been utilized in Western blotting, with specific dilutions reported in various studies, indicating its effectiveness for detecting Cre recombinase in protein extracts .

What species does this antibody react with?

The Cre-Recombinase antibody is designed to react with bacteria and archaea, as it targets the Cre protein derived from Bacteriophage P1 .

Synaptic Systems GmbH

Rudolf-Wissell-Str. 28

Goettingen 37079

Germany

Phone: +49-551-50556-0
+49-551-50556-384

Company Profile

Website: http://www.sysy.com/

(1) Dissociating the Hallucinogenic and Neuroplastic Effects of PsilocybinbioRxivApril 9, 2026Jacob J. Baker, Emily Kogan, Ju Lu, Yi Zuothen stained with a primary antibody against Cre recombinase (rabbit polyclonal, Synaptic Systems # 257 003, 1:1000) in a solution of 5% NGS in PBS at 4°C overnight. After rinsing in PBS, slices were stained

(2) Distinct CA1 inputs support shifts in neural dimensionality and memory resolutionbioRxivJanuary 20, 2026Cory McKenzie, Arna Ghosh, Adam I. Ramsaran, Bozhi Wu, Sakthivel Srinivasan, Tianwei Liu, Sheena A. Josselyn, Blake Richards, Paul W. Frankland600-401-379), mouse anti-PV (1:1000, Sigma, cat# 1572), rabbit anti-SST (1:250, Synaptic Systems, cat#257003) for 24 h or 48 h (for c-Fos and PV experiments) at 4 °C. Sections were washed three times for 15 min

(3) Juvenile reinstatement of TCF4 in Pitt-Hopkins syndrome model mice reveals a critical window for genetic interventionbioRxivDecember 26, 2025Lucas M. James, Carlee A. Friar, Siyuan Liang, Eric B. Gao, Alain. C. Burette, Benjamin D. Philpottemperature (RT). Sections were then incubated with Cre-recombinase primary antibody (Synaptic Systems, #257003, 1:500) in 10% FBS in PBST for 24 hours at RT. Sections were then washed in PBST and incubated for 24

(4) Deletion of Endocannabinoid Synthesizing Enzyme DAGLα in Pcp2-Positive Cerebellar Purkinje Cells Decreases Depolarization-Induced Short-Term Synaptic Plasticity, Reduces Social Preference, and Heightens AnxietyeNeuroJune 16, 2025Gabriella Smith, Kathleen McCoy, Gonzalo Viana Di Prisco, Alexander Kuklish, Emma Grant, Mayil Bhat, Sachin Patel, Ken Mackie, Brady Atwood, Anna Kalinovsky(Katona et al., 2006)",AB_2813825,"1:1,000" Rabbit anti Cre,"Synaptic Systems, polyclonal, catalog #257003",AB_2619968,1:200 Mouse anti Plcβ4,"Santa Cruz Biotechnology, monoclonal, clone A-8, catalog #sc-166131",AB_2237003,1:500 Rabbit

(5) Deletion of Endocannabinoid Synthesizing Enzyme DAGLα in Pcp2-Positive Cerebellar Purkinje Cells Decreases Depolarization-Induced Short–Term Synaptic Plasticity, Reduces Social Preference, and Heightens AnxietyeNeuroJune 13, 2025Gabriella Smith, Kathleen McCoy, Gonzalo Viana Di Prisco, Alexander Kuklish, Emma Grant, Mayil Bhat, Sachin Patel, Ken Mackie, Brady Atwood, Anna Kalinovsky(Katona et al., 2006)",AB_2813825,"1:1,000" Rabbit anti Cre,"Synaptic Systems, polyclonal, catalog #257003",AB_2619968,1:200 Mouse anti Plcβ4,"Santa Cruz Biotechnology, monoclonal, clone A-8, catalog #sc-166131",AB_2237003,1:500 Rabbit

(6) A Study on Potential Sources of Perineuronal Net-Associated Sema3A in Cerebellar Nuclei Reveals Toxicity of Non-Invasive AAV-Mediated Cre Expression in the Central Nervous SystemInternational journal of molecular sciencesJanuary 19, 2025Gimenez GA, Romijn M, van den Herik J, Meijer W, Eggers R, Hobo B, De Zeeuw CI, Canto CB, Verhaagen J, Carulli D.chicken anti-GFP (1:1000, #AB16901, Millipore, Merck, Darmstadt, Germany,), rabbit anti-Cre (1:1500, #257 003, Synaptic Systems, Goettingen, Germany) [18,54,55,56], Mouse anti-calbindin (1:1500, #300, Swant, Burgdorf,

(7) Cell-type-specific representation of spatial context in the rat prefrontal cortexiScienceMay 17, 2024Hans Brünner, Hoseok Kim, Sofie Ährlund-Richter, Josina Anna van Lunteren, Ana Paula Crestani, Konstantinos Meletis, Marie Carlénthereafter incubated with primary antibodies (1:250 Cre-recombinase, rabbit, Synaptic Systems, cat. no. 257 003, and 1:1000 Parvalbumin, guinea pig, Swant, code no. GP72, or Parvalbumin, guinea pig, Synaptic Systems,

(8) Context-dependent requirement of G protein coupling for Latrophilin-2 in target selection of hippocampal axonseLifeMarch 20, 2023Pederick DT, Perry-Hauser NA, Meng H, He Z, Javitch JA, Luo L.anti-mCherry (1:1000, ThermoFisher, M11217, RRID:AB_2536611), rabbit anti-Cre (1:500, Synaptic Systems, 257 003, RRID:AB_2619968), chicken anti-GFP (1:2500, Aves Labs, GFP-1020, RRID:AB_10000240) goat anti-FLAG (1:3000,

(9) CYFIP2 p.Arg87Cys Causes Neurological Defects and Degradation of CYFIP2Annals of neurologyJanuary 1, 2023Kang M, Zhang Y, Kang HR, Kim S, Ma R, Yi Y, Lee S, Kim Y, Li H, Jin C, et al.

(10) A critical period of translational control during brain development at codon resolutionNature structural & molecular biologyDecember 1, 2022Harnett D, Ambrozkiewicz MC, Zinnall U, Rusanova A, Borisova E, Drescher AN, Couce-Iglesias M, Villamil G, Dannenberg R, Imami K, et al.

(11) The role of α-tubulin tyrosination in controlling the structure and function of hippocampal neuronsFrontiers in molecular neuroscienceJanuary 1, 2022Hosseini S, van Ham M, Erck C, Korte M, Michaelsen-Preusse K.Systems Cat# 302 117, RRID:AB_2620047), an anti-Cre recombinase antibody (1:500, Synaptic Systems Cat# 257 003, RRID:AB_2619968), an anti-MAP-2 antibody (1:500, Synaptic Systems Cat# 188 011, RRID:AB_2147096), and

(12) Npas1⁺-Nkx2.1⁺ Neurons Are an Integral Part of the Cortico-pallido-cortical LoopThe Journal of neuroscience : the official journal of the Society for NeuroscienceJanuary 22, 2020Abecassis ZA, Berceau BL, Win PH, García D, Xenias HS, Cui Q, Pamukcu A, Cherian S, Hernández VM, Chon U, et al.

(13) Regulation of BDNF Release by ARMS/Kidins220 through Modulation of Synaptotagmin-IV LevelsThe Journal of neuroscience : the official journal of the Society for NeuroscienceJune 6, 2018López-Benito S, Sánchez-Sánchez J, Brito V, Calvo L, Lisa S, Torres-Valle M, Palko ME, Vicente-García C, Fernández-Fernández S, Bolaños JP, et al.rabbit ␤-tubulin III polyclonal (T8328, SigmaAldrich), 1:10,000 for immunoblot; rabbit Cre polyclonal (257003, Synaptic Systems), 1:750 for immunoblot and 1:500 for immunohistochemistry; rabbit Syt-IV polyclonal

(14) Satb2^Cre/+ mouse as a tool to investigate cell fate determination in the developing neocortexJournal of neuroscience methodsNovember 1, 2017Ambrozkiewicz MC, Bessa P, Salazar-Lázaro A, Salina V, Tarabykin V.

(15) [Unemployment and utilization of the public health system]Das Offentliche GesundheitswesenDecember 1, 1988M J Studnicka, V Scheiber

(16) Polarity Acquisition in Cortical Neurons Is Driven by Synergistic Action of Sox9-Regulated Wwp1 and Wwp2 E3 Ubiquitin Ligases and Intronic miR-140NeuronJanuary 1, 0001"Ambrozkiewicz MC, Schwark M, Kishimoto-Suga M, Borisova E, Hori K, Salazar-Lázaro A, Rusanova A, Altas B, Piepkorn L, Bessa P, et al."

(17) A divergent astrocytic response to stress alters activity patterns via distinct mechanisms in male and female miceNature communicationsJanuary 1, 0001"Depaauw-Holt LR, Duquenne M, Hamane S, Peyrard S, Rogers B, Ireland C, Nasu Y, Fulton S, Bosson A, Alquier T, et al."[rabbit] anti-Glutamine Synthetase (1:500, Abcam, ab73593), [rabbit] anti-Cre (1:500, Synaptic Systems, 257 003). After primary antibody incubation slices were washed 3 times in 1X PBS for 15 min to remove non-specific

(18) Cerebellin-2 regulates a serotonergic dorsal raphe circuit that controls compulsive behaviorsMolecular psychiatryJanuary 1, 0001"Seigneur E, Wang J, Dai J, Polepalli J, Südhof TC."anti-6xHis (1:200; 75-169 NeuroMab; RRID: AB_10673446), polyclonal rabbit anti-Cre-Recombinase (1:250, 257 003 Synaptic Systems; RRID: AB_2619968), polyclonal chicken anti-GFP (1:1000; GFP-1020 Aves Labs; RRID:

(19) Human OPRM1 and murine Oprm1 promoter driven viral constructs for genetic access to μ-opioidergic cell typesNature communicationsJanuary 1, 0001"Salimando GJ, Tremblay S, Kimmey BA, Li J, Rogers SA, Wojick JA, McCall NM, Wooldridge LM, Rodrigues A, Borner T, et al."NBP1-97371], rabbit anti-Iba1 [1:1000, Wako, 019-19741], rabbit anti-Cre [1:1000, Synaptic Systems, 257 003], rabbit anti-MOR [1:500, Abcam, ab134054], mouse anti-CC1/anti-APC [1:200, Millipore Sigma, Op80],

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Cre-Recombinase Antibody

Cre-Recombinase Antibody

Cre-Recombinase Antibody

Cre-Recombinase Antibody

Cre-Recombinase Antibody from Synaptic Systems GmbH

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Cre-Recombinase Antibody from Synaptic Systems GmbH

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