Fig 1: PRPS1 overexpression increased cell growth and decreased apoptosis of B-ALL cells. (A) PRPS1 overexpressing Sup-b15 and Raji cells exhibited increased cell growth compared with the vector control group. (***P<0.001). (B) Flow cytometric cell counts of PRPS1 overexpressing and vector control Sup-b15 and Raji cells stained with annexin V-APC/7AAD. (C) Apoptotic rate of Sup-b15 and Raji cells overexpressing PRPS1 or not; PRPS1 overexpression significantly decreased the apoptosis of B-ALL cells. (**P<0.01). PRPS1+, cells overexpressing phosphoribosyl pyrophosphate synthetase 1; B-ALL, B-cell acute lymphoblastic leukemia; APC, allophycocynanin; 7AAD, 7-aminoactinomycin.
Fig 2: PRPS1 expression in Sup-b15 and Raji cells transfected with lentivirus. (A and B) PRPS1 mRNA levels detected by reverse transcription-quantitative polymerase chain reaction in the (A) Sup-b15 and (B) Raji cells infected with empty vector control and PRPS1-overexpressing lentivirus, respectively (***P<0.001). (C and D) PRPS1 protein levels detected by western blotting in (C) Sup-b15 and (D) Raji cells infected with empty vector control and PRPS1-overexpressing lentivirus, respectively. β-actin level was used as endogenous control. PRPS1+, cells overexpressing phosphoribosyl pyrophosphate synthetase 1.
Fig 3: PRPS1 was highly expressed in samples from patients newly diagnosed with acute leukemia. (A) PRPS1 expression was detected by reverse transcription-quantitative polymerase chain reaction in different group of samples and was increased in B-ALL patients and was associated with disease progression and treatment status (***P<0.001). (B) The representative figure of PRPS1 proteins in samples of B-ALL and control patients stained by immunohistochemical staining (magnification, ×100). PRPS1, phosphoribosyl pyrophosphate synthetase 1; CR, complete remission; B-ALL, B-cell acute lymphoblastic leukemia.
Fig 4: PRPS1 overexpression significantly increased the expression of Bcl-2. (A) mRNA expression of CDK2, cyclin E1, c-Myc and Bcl-2 detected by reverse transcription-quantitative polymerase chain reaction in PRPS1 overexpressing and vector control Sup-b15 and Raji cells; the mRNA expression of Bcl-2 was increased by PRPS1 overexpression. (**P<0.01; ***P<0.001). (B) The protein expression of CDK2, Cyclin E1, c-Myc and Bcl-2 detected by western blotting in PRPS1 overexpressing and vector control Sup-b15 and Raji cells; the level of Bcl-2 protein was increased by PRPS1 overexpression. PRPS1+, cells overexpressing phosphoribosyl pyrophosphate synthetase 1; Bcl-2, B-cell lymphoma 2; c-Myc, MYC proto-oncogene, bHLH transcription factor; CDK2, cyclin-dependent kinase 2.
Fig 5: PRPS1 expression level was associated with risk stratification of B-ALL. PRPS1 expression was detected by reverse transcription-quantitative polymerase chain reaction in B-ALL patients grouped with diseases and risk stratification. **P<0.01; ***P<0.001. PRPS1, phosphoribosyl pyrophosphate synthetase 1; B-ALL, B-cell acute lymphoblastic leukemia SR, standard risk; IR, intermediate risk; HR, high risk.
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