Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 from Abcam

Product Specs
ItemGoat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab150081
Quantity500 UG
ApplicationsELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections)
Conjugate/TagAlexa Fluor® 488
FormatAntiserum was cross adsorbed using a human, mouse and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads
IsotypeIgG
HostGoat
Antibody TypeSecondary
Concentration2 mg/ml
ClonalityPolyclonal
TargetIgG

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) The Hexane Extract of Citrus sphaerocarpa Ameliorates Visceral Adiposity by Regulating the PI3K/AKT/FoxO1 and AMPK/ACC Signaling Pathways in High-Fat-Diet-Induced Obese MiceMoleculesDecember 9, 2023Liqing Zang, Kazuhiro Kagotani, Takuya Hayakawa, Takehiko Tsuji, Katsuzumi Okumura, Yasuhito Shimada, Norihiro Nishimurafluorescence-conjugated secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed (ab150081; Abcam, Cambridge, MA, USA) at room temperature (25 °C) for 1 h and mounted using ProLong Gold Antifade Mountant with DAPI

(2) Orange-derived extracellular vesicles nanodrugs for efficient treatment of ovarian cancer assisted by transcytosis effectActa Pharmaceutica Sinica BDecember 1, 2023Feng Long, Yao Pan, Jinheng Li, Suinan Sha, Xiubo Shi, Haoyan Guo, Chuanqing Huang, Qian Xiao, Chao Fan, Xingmei Zhang, et al.(ab137029), Rab11 (ab65200) primary antibodies and Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) were purchased from Abcam company (USA). Horseradish peroxidase (HRP)-conjugated anti-rabbit secondary

(3) The survivin-ran inhibitor LLP-3 decreases oxidative phosphorylation, glycolysis and growth of neuroblastoma cellsBMC CancerNovember 25, 2023Celimene Galiger, Fatema Tuj Zohora, Carmen Dorneburg, Daniel Tews, Klaus-Michael Debatin, Christian Beltinger°C. Cells were washed with PEMT buffer before incubation with Alexa 488 goat-anti-rabbit IgG, Abcam, ab150081, 1:1000), Alexa 568 goat-anti-mouse IgG (Thermo Fisher Scientific, A-11031, 1:1000) and DAPI (Molecular

(4) Annexin A1 exerts analgesic effect in a mouse model of medication overuse headacheiScienceNovember 17, 2023Zihua Gong, Chunxiao Yang, Wei Dai, Shuai Miao, Yingyuan Liu, Zhiyang Jiao, Bozhi Li, Wei Xie, Wei Zhao, Xun Han, et al.fluor-488, donkey anti-goat or goat anti-mouse IgG conjugated with Alex fluor-594 (1:1000, abcam, ab150061, ab150081, ab150136, ab150120, Waltham, MA, USA) for 2 h at room temperature. After washing thrice with PBST,

(5) Site-selected in situ polymerization for living cell surface engineeringNature CommunicationsNovember 10, 2023Yihong Zhong, Lijia Xu, Chen Yang, Le Xu, Guyu Wang, Yuna Guo, Songtao Cheng, Xiao Tian, Changjiang Wang, Ran Xie, et al.secondary antibody goat anti-rabbit IgG H&L (Alexa Fluor 488, pre-adsorbed, 1:1000 dilution, Abcam #ab150081) for 30 min at r.t. Cells were collected by centrifugation, washed three times in 1% FBS-containing

(6) Repurposing dimethyl fumarate as an antiepileptogenic and disease-modifying treatment for drug-resistant epilepsyJournal of Translational MedicineNovember 8, 2023Sereen Sandouka, Prince Kumar Singh, Aseel Saadi, Rhoda Olowe Taiwo, Yara Sheeni, Taige Zhang, Larin Deeb, Michelle Guignet, Steve H. White, Tawfeeq Shekh-Ahmadeach, the sections were incubated with Alexa Fluor® 488 goat anti-rabbit secondary antibody (1:500; ab150081, Abcam, Cambridge, UK) for 2 h at room temperature in the dark. The sections were washed three times

(7) Therapeutic potential of Lianhua Qingke in airway mucus hypersecretion of acute exacerbation of chronic obstructive pulmonary diseaseChinese MedicineNovember 3, 2023Yuanjie Hao, Tongxing Wang, Yunlong Hou, Xiaoqi Wang, Yujie Yin, Yi Liu, Ningxin Han, Yan Ma, Zhen Li, Yaru Wei, et al.PBS and incubated with the secondary antibodies of goat anti-rabbit IgG H&L (Alexa Fluor® 488, Abcam, ab150081,1:500), goat anti-mouse IgG (H + L) (CoraLite594, Proteintech, SA00013-3, 1:500), Goat Anti-Rat IgG

(8) A non-human primate derived anti-P-selectin glycoprotein ligand-1 antibody curtails acute pancreatitis by alleviating the inflammatory responsesActa Pharmaceutica Sinica BNovember 1, 2023Yuhan Li, Xiangqing Ding, Xianxian Wu, Longfei Ding, Yuhui Yang, Xiaoliang Jiang, Xing Liu, Xu Zhang, Jianrong Su, Jianqing Xu, et al.and anti CD-68 (Abcam, #ab201340), followed by goat anti-rabbit IgG H&L (Alexa Fluor® 488) (Abcam, #ab150081) or goat anti-mouse IgG H&L (Alexa Fluor® 568) (Abcam, #ab175473) antibody at 1:2000 dilution and the

(9) Exosomes Derived from M2 Microglial Cells Modulated by 1070‐nm Light Improve Cognition in an Alzheimer's Disease Mouse ModelAdvanced ScienceNovember 1, 2023Chengwei Chen, Yuting Bao, Lu Xing, Chengyong Jiang, Yu Guo, Shuangmei Tong, Jiayi Zhang, Liang Chen, Ying Maowashed three times with PBS for 10 min each time and subsequently reacted with Alexa Fluor 488 (1:1000, ab150081, Abcam), Alexa Flour 594 (1:1000, ab150120, Abcam), or Alexa Fluor 647 (1:1000, ab150083, Abcam) secondary

(10) Endotenon-Derived Type II Tendon Stem Cells Have Enhanced Proliferative and Tenogenic PotentialInternational Journal of Molecular SciencesOctober 12, 2023Marta Clerici, Vera Citro, Amy L. Byrne, Tina P. Dale, Aldo R. Boccaccini, Giovanna Della Porta, Nicola Maffulli, Nicholas R. Forsythtimes with PBS and a secondary antibody (Goat Anti-Rabbit IgG H&L Alexa Fluor® 48, pre-adsorbed) (Abcam ab150081) was added (dilution 1:500) and incubated in the dark for 1 h at room temperature. After incubation,

(11) EYA4 promotes breast cancer progression and metastasis through its role in replication stress avoidanceMolecular CancerSeptember 30, 2023Bárbara de la Peña Avalos, Romain Tropée, Pascal H. G. Duijf, Eloïse Dray(H-260; Santa Cruz sc-22791), γH2AX (S139) (Millipore 05–636). Secondary antibodies: α-Rabbit (Abcam ab150081, Alexa Fluor 488), α-Mouse (Abcam ab150103, Alexa Fluor 647). Nuclear foci quantification was performed

(12) Engineered bone marrow as a clinically relevant ex vivo model for primary bone cancer research and drug screeningProceedings of the National Academy of SciencesSeptember 26, 2023Katherine H. Griffin, Steven W. Thorpe, Aimy Sebastian, Nicholas R. Hum, Thomas P. Coonan, Isabel S. Sagheb, Gabriela G. Loots, R. Lor Randall, J. Kent Leachtemperature. Slides were then treated with a secondary goat anti-rabbit antibody conjugated to AF488 (ab150081, Abcam) at a concentration of 1:300 for 1 h at room temperature. CD31 slides were counterstained with

(13) CTGF promotes the repair and regeneration of alveoli after acute lung injury by promoting the proliferation of subpopulation of AEC2sRespiratory researchSeptember 23, 2023Sun J, Zhang H, Liu D, Liu W, Du J, Wen D, Li L, Zhang A, Jiang J, Zeng L.(Invitrogen, L7526), and p63 (Abcam, ab735, 1:200). The secondary antibodies were Alexa Fluor 488 (Abcam, ab150081, 1:400) and Alexa Fluor 594 (Abcam, ab150120, 1:400). Images were obtained using a Zeiss confocal microscope

(14) Human umbilical cord-derived mesenchymal stem cells ameliorate perioperative neurocognitive disorder by inhibiting inflammatory responses and activating BDNF/TrkB/CREB signaling pathway in aged miceStem Cell Research & TherapySeptember 21, 2023Penghui Wei, Min Jia, Xiangyi Kong, Wenyuan Lyu, Hao Feng, Xinyi Sun, Jianjun Li, Jian-jun Yanganti-rabbit Alexa Fluor® 647 (1:500, Abcam, ab150083), goat anti-rabbit Alexa Fluor® 488 (1:500, Abcam, ab150081), goat anti-mouse Alexa Fluor® 488 (1:500, Abcam, ab150117) or goat anti-mouse Alexa Fluor® 647 (1:500,

(15) An in vivo model of ligamentum flavum hypertrophy from early‐stage inflammation to fibrosisJOR SPINESeptember 1, 2023Kevin G. Burt, Dan C. Viola, Lauren E. Lisiewski, Joseph M. Lombardi, Louis F. Amorosa, Nadeen O. Chahineroom temperature with secondary fluorescent antibodies goat anti‐rabbit Alexa Fluor (AF) 488 (Abcam, AB150081, 1:100) or goat anti‐mouse AF595 (Invitrogen, A‐11005, 1:100). Sections were mounted with VECTASHEILD

(16) Esculetin Alleviates Inflammation, Oxidative Stress and Apoptosis in Intestinal Ischemia/Reperfusion Injury via Targeting SIRT3/AMPK/mTOR Signaling and Regulating AutophagyJ Inflamm ResAugust 23, 2023Xin Shen, Hai Shi, Xinli Chen, Junwei Han, Haiwang Liu, Jie Yang, Yuan Shi, Jiajia MaAbcam) overnight at 4°C. On the second day, Alexa Fluor® 488 goat anti-rabbit IgG antibody (cat. no. ab150081; Abcam) was added and incubated for another 1 h at room temperature. Nuclear DNA was labeled with DAPI.

(17) Astrocyte senescence-like response related to peripheral nerve injury-induced neuropathic painCellular & Molecular Biology LettersAugust 15, 2023Jingyi Du, Nan Cheng, Yifan Deng, Ping Xiang, Jianfen Liang, Zhenye Zhang, Ziqing Hei, Xiang LiCell Signaling Technology). Then, the cultured astrocytes and spinal tissues were incubated with Alexa 488-conjugated (1:500, ab150081, Abcam) or Alexa 594-conjugated (1:500, ab150120, Abcam) secondary antibody and treated with 4′,6-diamidino-2-phenylindole

(18) Physicochemical Nature of SARS-CoV-2 Spike Protein Binding to Human VimentinACS Applied Materials & InterfacesJuly 19, 2023Piotr Deptuła, Krzysztof Fiedoruk, Monika Wasilewska, Łukasz Suprewicz, Mateusz Cieśluk, Paulina Żeliszewska, Magdalena Oćwieja, Zbigniew Adamczyk, Katarzyna Pogoda, Robert Buckialbumin (BSA) in PBS was added for 30 min at RT. A secondary antirabbit Alexa Fluor 488 antibody (Abcam, #ab150081) was used at 1:1000 dilution for 1 h at RT in the dark. Nuclei were counterstained with NucBlue Live ReadyProbes Reagent

(19) Melatonin regulates the periodic growth of secondary hair follicles through the nuclear receptor RORαFrontiers in Veterinary ScienceJuly 10, 2023Zeyu Lu, Jing Wu, Jing Wu, Tiejia Zhang, Junyang Liu, Qing Mu, Terigele, Zixian Wu, Yanjun Zhang, Rui Su, et al.incubated with 0.01 mol/L secondary antibody [Goat Anti-Rabbit IgG H&L (Alexa Fluor®) preabsorbed, ab150081, Abcam, America] containing 0.4% Triton prepared with PBS at room temperature for 1 h. Rinse with 0.01

(20) Dim artificial light at night alters immediate early gene expression throughout the avian brainFrontiers in NeuroscienceJuly 4, 2023Cassandra K. Hui, Nadya Chen, Arunima Chakraborty, Valentina Alaasam, Simon Pieraut, Jenny Q. Ouyangat room temp and incubated overnight at 4° C with secondary antibodies (anti-rabbit 488 (from ABCAM ab150081) diluted 1:1000 and anti-mouse 594 (from ABCAM ab150116) diluted 1:1000), protected from light. We then

Write a Review

(1)

6/23/2020This Anti-Rabbit Secondary Antibody Works Extremely Well Samantha LanjewarGreat secondary antibody!

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed from Abcam

Abcam

Citations (177)

Reviews

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map