Fig 1: The shASRGL1 suppressed C666-1 and SUN-1 cell proliferation viability and division. A ASRGL1 RNA interference genes were tested. The mRNA expression level of ASRGL1 was measured by RT-qPCR. Relative mRNA levels were compared in different groups, two-tailed, unpaired t-test, ***p < 0.001. B The protein expression level of ASRGL1 was measured by WB. Relative protein levels were compared in different groups, two-tailed, unpaired t-test, ***p < 0.001. C Cell proliferation was measured using a CCK-8 assay. The cell viability of the control group and the shASRGL1 group was compared by measuring the detection absorbance at 450 nm at different time points. Two-way ANOVA, ***p < 0.001, D The percentage of cell population in different phases of the cell cycle was quantified and compared between the control group and the shASRGL1 group. Two-tailed, unpaired t-test, *p < 0.05, *p < 0.01, ***p < 0.001
Fig 2: ASRGL1 was highly expressed in Nasopharyngeal carcinoma tissues and cell lines. A The mRNA expression level of ASRGL1 in NPC patients was detected by RT-qPCR. Relative mRNA levels were compared in different groups, two-tailed, unpaired t-test, ***p < 0.001. B The protein expression level of ASRGL1 in NPC patients was detected by WB. Representative bands for the 5 cases are shown in the figure. Relative protein levels were compared in different groups, two-tailed, unpaired t-test, *p < 0.05. C The mRNA expression level of ASRGL1 in NPC cell lines was detected by RT-qPCR. Relative mRNA levels were compared in different groups. One-way ANOVA, **p < 0.01, ***p < 0.001. D The protein expression level of ASRGL1 in NPC cell lines was detected by WB. Relative protein levels were compared in different groups. One-way ANOVA, ***p < 0.001
Supplier Page from Abcam for Anti-ASRGL1 antibody