Western blot was performed using CTH Polyclonal Antibody (Product # PA5-29725) and a 42 kDa, 39 kDa band corresponding to CTH was observed in tested cell lines and tissue positive (liver) for CTH respectively. Whole cell extracts (30 µg lysate) of BeWo (Lane 1), K 562 (Lane 2), tissue extracts (30 µg lysate) of Mouse liver (Lane 3), Mouse testis (Lane 4) and Mouse prostate (Lane 5) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).