Immunofluorescent analysis of CREM in paraformaldehyde-fixed HeLa cells using a CREM polyclonal antibody (Product # PA5-29927) at a 1:200 dilution.

Western blot was performed using Anti-CREM Polyclonal Antibody(Product # PA5-29927) and a 37 kDa band corresponding to CREM was observed across the cell lines and tissues tested. Whole Cell Extract (1% SDS), (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), Mouse Brain (Lane 4), Mouse Testis (Lane 5) and Mouse Kidney (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).