Knockdown of Interferon-induced protein with tetratricopeptide repeats 1 was achieved by transfecting HeLa treated with Human IFN Beta (1000 U/mL for 24hrs) with Interferon-induced protein with tetratricopeptide repeats 1 specific siRNAs (Silencer® select Product # s7150, s7151). For detectable levels of IFIT1, transfected cells were treated with Human IFN Beta. Western blot analysis (Fig. a) was performed using Whole cell extracts from the Interferon-induced protein with tetratricopeptide repeats 1 knockdown cells treated with Human IFN Beta (lane 6), IFIT3 control HeLa cells (Lane 5), non-targeting scrambled siRNA transfected HeLa cells treated with Human IFN Beta (lane 4), non-targeting scrambled siRNA transfected HeLa cells (lane 3), Untransfected HeLa cells treated with Human IFN Beta (Lane 2) and untransfected HeLa cells (lane 1).The blot was probed with IFIT1 Polyclonal Antibody (Product # PA5-31254, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Interferon-induced protein with tetratricopeptide repeats 1.