Immunofluorescent analysis of CD36 in paraformaldehyde-fixed mouse ESC D3 cells using a CD36 polyclonal antibody (Product # PA5-27236) at a 1:200 dilution.

Western Blot was performed using Anti-CD36 Polyclonal Antibody (Product # PA5-27236) and a 88 kDa band corresponding to Platelet glycoprotein 4 was observed across tissues tested and the expression was found to be higher in adipose and brown adipose tissues in comparison to other tissues tested. Tissue extracts (30 µg lysate) of Mouse Adipose (Lane 1), Mouse Brown Adipose (Lane 2), Rat Adipose (Lane 3), Rat Brown Adipose (Lane 4), Mouse Brain (Lane 5), Rat Brain (Lane 6), Mouse Kidney (Lane 7), Mouse Liver (Lane 8), Rat Testis (Lane 9), Mouse Cerebellum (Lane 10), Mouse Colon (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Additional uncharacterized bands were also observed between 30-50 kDa.