Flow Cytometry analysis of TLR4 in formaldehyde-fixed THP-1 cells (green) compared to an isotype control (blue). Staining was performed using a TLR4 polyclonal antibody (Product # PA5-23124) at a dilution of 20µg x 10^6 cells, followed by secondary detection using a Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate (Product # A-11008) at a dilution of 1:200. Rabbit IgG Isotype Control (Product # MA5-16385) was used as a negative control at the same dilution as the primary antibody. Data provided courtesy of Antibody Resource.
Flow cytometry analysis of KDEL/ER was done on HeLa cells. Cells were fixed, permeabilized and stained with a KDEL/ER rabbit polyclonal antibody (Product # PA1-013, purple histogram) or a rabbit IgG isotype control (Product # MA5-16384, black histogram) at a dilution of 10 µg/mL. After incubation for 1 hour on ice, the cells were labeled with a Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 647 conjugate (Product # A27040) at a dilution of 1:50 for 1 hour on ice. A representative 10,000 cells were acquired and analyzed for each sample.
Supplier Page from Thermo Fisher Scientific for Rabbit IgG Isotype Control