PD-L1 Antibody PA5-28115 from Thermo Fisher Scientific

Product Specs
ItemPD-L1 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-28115
Quantity100 µL
ApplicationsFlow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Conjugate/TagUnconjugated
FormatPBS with 20% glycerol and 0.025% Proclin 300; pH 7
ReactivityHuman
ClonalityPolyclonal
TargetPD-L1
IsotypeIgG
HostRabbit
NCBI Full Gene NameCD274 molecule
NCBI Gene AliasesB7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1
Concentration0.97 mg/mL
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles
ImmunogenSynthetic peptide encompassing a sequence within the Intracellular domain of human PD-L1. The exact sequence is proprietary

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Waltham, MA 02451

United States

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Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Butyrate enhances tumor immune evasion by increasing PD-L1 abundanceInt J Biol SciJuly 20, 2026Xue Sun, Yi Gao, Jiaxi Li, Yan Ma, Hongchen Li, Zhen Wang, Lei Lv, Yanping Xuobtain the sample for WB. The antibodies used in this study include PD-L1 (CST, 51296; Invitrogen, PA5-28115), mouse-PD-L1 (Abcam, ab213480), TGM2 (Proteintech, 15100-1-AP), importin α3 (Proteintech, 12463-1-AP),

(2) The role of amino bisphosphonates zoledronate and alendronate in shaping immunomodulatory profile and angiogenic potential of human periodontal ligament stem cellsPLoS OneDecember 1, 2025Jessica Bria, Emanuela Chiarella, Jessica Sovereto, Francesco Baudi, Marta Greco, Daniela Patrizia Foti, Amerigo Giudice, Anna Di Vitoblock the nonspecific binding sites. Treated and untreated cells were incubated with anti-PD-L1 (no. PA5–28115; 1:200, rabbit; ThermoFisher Scientific) for 16 h (4°C) and then with Alexa-Fluor 488-conjugated anti-rabbit

(3) MINDY1 Induces PD-L1 Deubiquitination to Promote Immune Escape in Hepatocellular Carcinoma by the Wnt/β-Catenin PathwayOncology ResearchOctober 22, 2025Xingchao Song, Qiuyu Song, Xiao Ma, Anzhi Xu, Chunyan Tiantreated with RIPA lysis buffer for 1 h. The resulting lysate supernatant was mixed with anti-PD-L1 (PA5-28115, 1:500) at 4°C for 2 h, with immunoglobulin G (IgG, MA5-42729, 1:100) as a control. The above antibodies

(4) Combination therapy with Chicoric acid and PD-1/PD-L1 blockade improves the immunotherapy response in patient-derived ovarian cancer xenograft modelCell Commun SignalMarch 14, 2025Hongwei Lan, Jingjuan Zhu, Helei Hou, Chuantao Zhang, Xingfa Huo, Yuming Zhang, Fangfang Yang, Na Zhou, Xiaochun Zhanganti-human CD8 alpha (ab205921, 1:1000 dilution; Abcam, Cambridge, UK) or rabbit anti-human PD-L1 (PA5-28115, 1:800 dilution; Invitrogen, USA) was added. The next steps were performed via the Mouse and Rabbit

(5) Immunological Tumor Microenvironment of Solitary Fibrous Tumors—Associating Immune Infiltrate with Variables of Prognostic SignificanceCancers (Basel)September 21, 2024Emilio Medina-Ceballos, Isidro Machado, Francisco Giner, Álvaro Blázquez-Bujeda, Mónica Espino, Samuel Navarro, Antonio Llombart-Bosch(KP1—Dako, Santa Clara, CA, USA), CD3 (Polyclonal Rabbit—Dako), CD8 (C8/144B—Dako), CD20 (L26—Dako), PDL-1 (PA5-28115—Thermo Fisher Scientific, Waltham, MA, USA), PD-1 (SP142—Roche, Basel, Switzerland), and LAG1 (Gennova

(6) Alignment, segmentation and neighborhood analysis in cyclic immunohistochemistry data using CASSATTCytometry Part B: Clinical CytometrySeptember 1, 2023Asa A. Brockman, Rohit Khurana, Todd Bartkowiak, Portia L. Thomas, Shamilene Sivagnanam, Courtney B. Betts, Lisa M. Coussens, Christine M. Lovly, Jonathan M. Irish, Rebecca A. IhrieCD68,Leica,PA0191,NA RTU anti CD3,Leica,PA0553,NA anti-PDCD1,Sigma-Aldrich,HPA035981,1:75 anti-PD-L1,ThermoFisher,PA5–28115,1:7500 anti-FoxP3,eBioscience,14–4777-82,1:100 anti-Iba1,FujiFilm,019–19741,1:1000 RTU anti CD4,Leica,AR9640,NA RTU

(7) Immunohistochemical Profiling of PD-1, PD-L1, CD8, MSI, and p53 and Prognostic Implications in Advanced Serous Ovarian Carcinoma: A Retrospective StudyJournal of personalized medicineJune 26, 2023Dergham AP, Vaz de Paula CB, Nagashima S, Olandoski M, de Noronha L, Sotomaior VS.primary antibodies for PD-1 (J116/14-9989-82; Thermo Fisher (Waltham, MA, USA); dilution 1:100), PD-L1 (PA5-28115; Thermo Fisher; dilution 1:200), CD8 (SP16; Thermo Fisher; dilution 1:100), and p53 (D07/BSB5844; BioSB;

(8) Iron-mediated oxidative stress induces PD-L1 expression via activation of c-Myc in lung adenocarcinomaFrontiers in cell and developmental biologyJanuary 1, 2023Battaglia AM, Sacco A, Aversa I, Santamaria G, Palmieri C, Botta C, De Stefano R, Bitetto M, Petriaggi L, Giorgio E, et al.at room temperature and incubated overnight at 4°C with primary antibodies against PD-L1 (1:1,000, PA5-28115, ThermoFisher Scientific), c-Myc (1:500, sc-42, Santa Cruz Biotechnology), Nrf2 (1:200, sc-365949, Santa Cruz Biotechnology

(9) Evaluation PD-L1, CD8 and CD20 as early predictor and tracking markers for breast cancer (BC) in EgyptHeliyonMay 1, 2022Hamed MM, Gouida MS, Abd El-Aziz SR, El-Sokkary AMA.peroxide 0.3% and protein block followed by incubation with PD-L1/CD274 polyclonal antibody (Product # PA5-28115) at a dilution of 1:100, Invitrogen, Zymed San Francisco CA (USA). The slides were rinsed three times

(10) Therapeutic and immunomodulatory potential of pazopanib in malignant phyllodes tumorNPJ breast cancerApril 1, 2022Ng DYX, Li Z, Lee E, Kok JST, Lee JY, Koh J, Ng CC, Lim AH, Liu W, Ng SR, et al.For PD-L1 staining, cells were incubated with PD-L1 antibody (1:100, 30 min at room temperature) (#PA5-28115, Thermo Fisher Scientific, MA, USA), washed and resuspended in PBS and marked with PE-conjugated secondary

(11) Deep Phenotypic Characterisation of CTCs by Combination of Microfluidic Isolation (IsoFlux) and Imaging Flow Cytometry (ImageStream)CancersDecember 20, 2021Ruiz-Rodríguez AJ, Molina-Vallejo MP, Aznar-Peralta I, González Puga C, Cañas García I, González E, Lorente JA, Serrano MJ, Garrido-Navas MC.

(12) Licorice extract inhibits growth of non-small cell lung cancer by down-regulating CDK4-Cyclin D1 complex and increasing CD8⁺ T cell infiltrationCancer cell internationalOctober 12, 2021Zhu J, Huang R, Yang R, Xiao Y, Yan J, Zheng C, Xiao W, Huang C, Wang Y.(1:50000, ab32053, Abcam), P21 (1:5000, ab109520, Abcam), PD-L1 (1:500, ab205921, Abcam or 1:2000, PA5-28115, Thermo Fisher scientific) and β-actin (1:2000, ab8227, Abcam); Secondary antibodies were goat anti-rabbit

(13) Enterovirus 71 Antagonizes Antiviral Effects of Type III Interferon and Evades the Clearance of Intestinal Intraepithelial LymphocytesFrontiers in microbiologyJanuary 1, 2021Dong Y, Liu J, Lu N, Zhang C.States). The following antibodies were used in this study. Rabbit anti-PD-L1 polyclonal antibody (Catalog# PA5-28115) and rabbit anti-MICA/B polyclonal antibody (Catalog# PA5-109323) were purchased from Invitrogen. Rabbit-anti-human

(14) MLLT6 maintains PD-L1 expression and mediates tumor immune resistanceEMBO reportsDecember 3, 2020Sreevalsan S, Döring M, Paszkowski-Rogacz M, Brux M, Blanck C, Meyer M, Momburg F, Buchholz F, Theis M.anti‐PD‐L1 (E1L3N) XP (Cell Signaling Technology, 13684), rabbit anti‐PD‐L1 (Thermo Fisher scientific, PA5‐28115), goat anti‐GAPDH (Acris Antibodies, AP16240PU‐N), rabbit anti‐IFNGR1 (Cell Signaling Technology, 34808),

(15) The deubiquitinase USP22 regulates PD-L1 degradation in human cancer cellsCell communication and signaling : CCSJuly 14, 2020Wang Y, Sun Q, Mu N, Sun X, Wang Y, Fan S, Su L, Liu X.regulating tumor immunosuppression. Methods Reagents and transfection The primary antibodies for PD-L1 (PA5–28115; Thermo Fisher Scientific) and (MAB90781; R&D SYSTEMS), USP22 (SC-390585; Santa Cruz), CSN5 (SC-13157;

(16) Rapidly fatal pneumonitis from immunotherapy and concurrent SARS-CoV-2 infection in a patient with newly diagnosed lung cancermedRxiv : the preprint server for health sciencesMay 1, 2020Lovly CM, Boyd KL, Gonzalez-Ericsson PI, Lowe CL, Brown HM, Hoffman RD, Sterling BC, Kapp ME, Johnson DB, Kopparapu PR, et al.

(17) A case report of clonal EBV-like memory CD4⁺ T cell activation in fatal checkpoint inhibitor-induced encephalitisNature medicineAugust 1, 2019Johnson DB, McDonnell WJ, Gonzalez-Ericsson PI, Al-Rohil RN, Mobley BC, Salem JE, Wang DY, Sanchez V, Wang Y, Chastain CA, et al.(StatLab, cat# RM27–10, prediluted), anti-CD8 (StatLab, cat# MM39–10, prediluted), anti-PD-L1 (Cat.#PA5–28115 ThermoFisher, Grand Island, NY; 1:7500), anti-CD68 (PA0191, Leica, Buffalo Grove, IL; prediluted), anti-PD-1

(18) HDAC6‑selective inhibitor synergistically enhances the anticancer activity of immunomodulatory drugs in multiple myelomaInternational journal of oncologyAugust 1, 2019Won HR, Lee DH, Yeon SK, Ryu HW, Kim GW, Kwon SH.1:1,000) was purchased from Aviva Systems Biology. Anti‑programmed death‑ligand 1 (PD‑L1) antibody (PA5‑28115; 1:1,000) was purchased from Invitrogen; Thermo Fisher Scientific, Inc. The membranes were then washed

(19) The Binding of PD-L1 and Akt Facilitates Glioma Cell Invasion Upon Starvation via Akt/Autophagy/F-Actin SignalingFrontiers in oncologyJanuary 1, 2019Chen RQ, Xu XH, Liu F, Li CY, Li YJ, Li XR, Jiang GY, Hu F, Liu D, Pan F, et al.phospho-P70S6K, LC3B were purchased from Cell Signaling Technology (MA, USA). Antibodies against PD-L1/CD274 (PA5-28115, Thermo Fisher, IL, USA), PD-L1 (ABM4E54, Abcam, Cambridge, UK), GST (Z-5, Santa Cruz Biotechnology,

(20) Immuno-PET Imaging of the Programmed Cell Death-1 Ligand (PD-L1) Using a Zirconium-89 Labeled Therapeutic Antibody, AvelumabMolecular imagingJanuary 1, 2019Jagoda EM, Vasalatiy O, Basuli F, Opina ACL, Williams MR, Wong K, Lane KC, Adler S, Ton AT, Szajek LP, et al.conditions: heat-induced epitope retrieval for 20 minutes and human-specific PD-L1 (Thermo, Catalog # PA5-28115) dilution 1:250 for 30 minutes. Positive control tissues included human thymus. Negative controls included

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PD-L1 Antibody from Thermo Fisher Scientific

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