Immunohistochemistry (Paraffin) analysis of RelB was performed in paraffin-embedded human breast carcinoma tissue using RelB Polyclonal Antibody (Product # PA5-27679) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western blot was performed using Anti-RelB Polyclonal Antibody (Product # PA5-27679) and a 63 kDa band corresponding to Transcription factor RelB was observed across across the cell lines tested. Whole cell extracts (30 µg lysate) of LNCaP (Lane 1), Raji (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10,000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).