Anti-PKA R2/PKR2 (phospho S99) antibody [E151] ab32390 from Abcam

Product Specs
ItemAnti-PKA R2/PKR2 (phospho S99) antibody [E151]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab32390
Antibody TypePrimary Antibody
IsotypeIgG
HostRabbit
ApplicationsICC/IF, IHC-P, WB
CloneE151
Quantity1 mL, 100 µL
ClonalityMonoclonal
Conjugate/TagUnconjugated
ModificationsPhospho-S99, Phospho-S99
Gene NamePRKAR2A
ReactivityHuman, Mouse, Pig, Rat
NCBI Gene AliasesPKR2, PRKAR2
Research AreaNeuroscience
TargetPRKAR2A phospho S99
NCBI Full Gene Nameprotein kinase cAMP-dependent type II regulatory subunit alpha

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Guilu erxian glue improves sperm quality in oligasthenospermia mice by promoting TDRP methylation and regulating the cAMP/PKA pathwaySci RepJuly 28, 2025Congxu Zhu, Yingqiu Li, Lumei Liu, Jing Xie, Zongren Hu, Qinghu He, Wen Shengincubated in the appropriately diluted primary antibody PKA (1: 1000, ab32514, Abcam), p-PKA (1: 1000, ab32390, Abcam), TDRP (1: 1000, PA5-52565, Thermo) or β-actin (1: 5000, 66009-1-Ig, Proteintech), while the

(2) Enhanced kinase translocation reporters for simultaneous real-time measurement of PKA, ERK, and calciumJournal of Biological ChemistryMarch 1, 2025Shang-Jui Tsai, Yijing Gong, Austin Dabbs, Fiddia Zahra, Junhao Xu, Aleksander Geske, Michael J. Caterina, Stephen J. Gouldincubated with primary antibodies for phospho-PKA (9101S, Cell Signaling Technology) and phospho-ERK (ab32390, Abcam). Specificity of these antibodies for phospho-PKA and phospho-ERK were established by the manufacturer

(3) Danggui-Shaoyao-San Can Ameliorate Alzheimer's Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum PharmacologyDrug Des Devel TherFebruary 10, 2025Kai-Xin Zhang, Ji-Wei Zhang, Yan-Hong Jiang, Yi-Ran Wang, Zhen-Ling Liu, Peng-Li Ding, Xiang-Ying Wang, Wen-Qiang Cui, Xiang-Qing Xu, Ya-Han Wangcovered with 3% bovine serum albumin(BSA) albumin and sealed for 30 mins. The prepared p-PKA (Abcam,ab32390,1:500) and p-CREB (Abcam,ab32096,1:100) antibodies were added to the well plate at 4 °C for the entire

(4) The Essential Role of Angiogenesis in Adenosine 2A Receptor Deficiency-mediated Impairment of Wound Healing Involving c-Ski via the ERK/CREB PathwaysInternational Journal of Biological SciencesAugust 19, 2024Yan Peng, Renping Xiong, Bo Wang, Xing Chen, Yalei Ning, Yan Zhao, Nan Yang, Jing Zhang, Changhong Li, Yuanguo Zhou, et al.Frontier Institute, AB_2571655), rabbit anti-Ski (1:100, Santa Cruz, sc-9140), anti-p-PKA (1:500, Abcam, ab32390), anti-p-ERK1/2 (1:200, Cell Signaling, 9101), and anti-p-CREB (1:500, Cell Signaling, 9198) at 4°C

(5) Protein kinase A is a functional component of focal adhesionsThe Journal of biological chemistryMay 1, 2024Kang M, Senatore AJ, Naughton H, McTigue M, Beltman RJ, Herppich AA, Pflum MKH, Howe AK.BD biosciences); Prx3 (PF-PA0255; Abfrontier); rabbit monoclonal anti–phospho-RII (pS99 clone E151, ab32390; Abcam); mouse anti-tubulin (DM1a; Sigma); anti-GAPDH (14C10; Cell Signaling Technologies); mouse anti-actin

(6) Excessive fat expenditure in MCT-induced heart failure rats is associated with BMAL1/REV-ERBα circadian rhythmic loop disruptionScientific ReportsApril 7, 2024Dufang Ma, Yiwei Qu, Tao Wu, Xue Liu, Lu Cai, Yong Wang(β-ACTIN) (1:1000, Cat No. 20536-1-AP, PROTEINTECH, Wuhan, China), protein kinase A (PKA) (1:1000, Cat No. ab32390, ABCAM, Cambridge, United Kingdom), phospho-PKA (p-PKA) (1:500, Cat No. Bs-3725r, BIOSS, Beijing, China),

(7) MPP2 interacts with SK2 to rescue the excitability of glutamatergic neurons in the BLA and facilitate the extinction of conditioned fear in miceCNS Neuroscience & TherapeuticsJanuary 1, 2024Xiaohan Peng, Panpan Chen, Yang Zhang, Ke Wu, Ningning Ji, Jinghua Gao, Hui Wang, Yong‐mei Zhang, Tie Xu, Rong Hua(1:500; Alomone labs, Israel), anti‐PKA (1:1000 Abcam ab38949), anti‐pPKA (phospho S96, 1:1000 Abcam ab32390), anti‐MPP2 (1:1000; Abcam, USA), anti‐GAPDH (1:1000), or anti‐β actin (1:3000) primary antibodies.

(8) C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophagesiScienceOctober 20, 2023Tian L, Li H, Zhao P, Liu Y, Lu Y, Zhong R, Jin Y, Tan T, Cheng Y.AB_2867012 Anti-PKA alpha/beta/gamma (WB),Abcam,Cat# ab75991;RRID: AB_1524202 Anti-PKA R2 (WB),Abcam,Cat# ab32390;RRID: AB_779040 Anti-GSK3 beta (WB),Abcam,Cat# ab32391;RRID: AB_2115066 Anti-GSK3 beta+alpha (WB),Abcam,Cat#

(9) Ketogenic diet ameliorates attention deficit hyperactivity disorder in rats via regulating gut microbiotaPLoS OneAugust 16, 2023Yu Liu, Changhong Yang, Yingxue Meng, Yonghui Dang, Lin Yangantibodies against DRD1 (1:1000; ab279713, Abcam, UK), DAT (1:1000; ab184451, Abcam, UK), PKA (1:5000; ab32390, Abcam, UK), DARPP32 (1:1000; ab40801, Abcam, UK), cAMP (1:20000; ab76238, Abcam, UK), and GAPDH (1:2000;

(10) Emergence of nociceptive functionality and opioid signaling in human induced pluripotent stem cell-derived sensory neuronsPainAugust 1, 2023Röderer P, Belu A, Heidrich L, Siobal M, Isensee J, Prolingheuer J, Janocha E, Valdor M, Hagendorf S, Bahrenberg G, et al.NB110-58872, only for DRG neurons), rabbit monoclonal anti-RIIα (phospho-Ser96) (1:1000, clone 151, Abcam, ab32390), and mouse monoclonal anti-PKA RIIβ (1:1000, BD Transduction Laboratories, Franklin Lakes, NJ, 610625).

(11) Astragalus polysaccharide prevents heart failure-induced cachexia by alleviating excessive adipose expenditure in white and brown adipose tissueLipids in Health and DiseaseJanuary 21, 2023Dufang Ma, Tao Wu, Yiwei Qu, Jinlong Yang, Lu Cai, Xiao Li, Yong Wang(1:2000, Cat No. ab181602, Abcam, Cambridge, United Kingdom), protein kinase A (PKA) (1:1000, Cat No. ab32390, Abcam, Cambridge, United Kingdom), phospho-PKA (p-PKA) (1:500, Cat No. Bs-3725r, Bioss, Beijing, China),

(12) GLP1R inhibits the progression of endometrial carcinoma through activation of cAMP/PKA pathwayJournal of clinical laboratory analysisOctober 1, 2022Li W, Gu Y, Liu S, Ruan F, Lv W.against GLP1R (1:500; Rabbit; bs‐1559R, 51 kDa, Bioss), phosphorylated‐PKA (p‐PKA; 1:1000; Rabbit; ab32390, 45 kDa, Abcam), PKA (1:10000; Rabbit; ab32514, 45 kDa, Abcam), and glyceraldehyde‐3‐phosphate dehydrogenase

(13) Inhibitory effect of PDE2 on inflammation and apoptosis in cerebral ischemia‑reperfusion injuryInternational Journal of Molecular MedicineJune 24, 2022Ding Wan, XIAOYAN WANG, Jin Feng, Peng Wang, Hua Xucleaved PARP (1:1,000; cat. no. ab32064); PARP (1:1,000; cat. no. ab191217); PKA (1:1,000; cat. no. ab32390) and GAPDH (1:1,000; cat. no. ab8245) all from Abcam, overnight at 4°C. The following day, the Goat Anti-Mouse IgG H&L (Alexa Fluor 647

(14) Ceftriaxone Suppresses Group II Metabotropic Glutamate Receptor Expression Contributing to Reversal of Recognition Memory Deficits of Amyloid Precursor Protein/Presenilin 1 AD MiceFrontiers in neuroscienceJanuary 1, 2022Fan S, Li L, Liu L, Li H, Xian X, Li W.United Kingdom, Cat: ab150387, Lot: GR105860-10, 1:1000 dilution), PKA (Abcam, United Kingdom, Cat: ab32390, Lot: GR31302-44, 1:1000 dilution), SNAP25 (Abcam, United Kingdom, Cat: ab5666, Lot: GR3255764-5, 1:1000

(15) AKAP79 enables calcineurin to directly suppress protein kinase A activityeLifeOctober 6, 2021Church TW, Tewatia P, Hannan S, Antunes J, Eriksson O, Smart TG, Hellgren Kotaleski J, Gold MG.,BD Biosciences,Cat# 610166; RRID:AB_397567,(0.8 μg/mL) Antibody,(Rabbit monoclonal) anti-PKA phospho-RIIα,Abcam,Cat# ab32390; RRID:AB_779040,(0.8 μg/mL) Antibody,(Rabbit polyclonal) anti-GFP,Sigma Aldrich,Cat# SAB4301138; RRID:AB_2750576,(0.5

(16) Depolarization induces nociceptor sensitization by CaV1.2-mediated PKA-II activationThe Journal of cell biologyOctober 4, 2021Isensee J, van Cann M, Despang P, Araldi D, Moeller K, Petersen J, Schmidtko A, Matthes J, Levine JD, Hucho T.(1:2,000; Novus; #NB110-58872), rabbit monoclonal anti-RIIα (phospho-Ser96; 1:1,000, clone 151; Abcam; #ab32390), mouse monoclonal anti-RIIβ (1:2,000; BD Transduction Laboratories; #610625), mouse monoclonal anti-NF200

(17) Serotonin enhances depolarizing spontaneous fluctuations, excitability, and ongoing activity in isolated rat DRG neurons via 5-HT₄ receptors and cAMP-dependent mechanismsNeuropharmacologyFebruary 15, 2021Lopez ER, Carbajal AG, Tian JB, Bavencoffe A, Zhu MX, Dessauer CW, Walters ET.Novus Biologicals, # NB110–58872), rabbit monoclonal anti-phospho RII (S99) (1:1000, clone 151, Abcam, # ab32390), and mouse anti-CGRP (1:1000, Santacruz #SC-57053). Secondary antibodies (all 1:1000): goat anti-chicken-DyLight

(18) Depolarization-Dependent C-Raf Signaling Promotes Hyperexcitability and Reduces Opioid Sensitivity of Isolated Nociceptors after Spinal Cord InjuryThe Journal of neuroscience : the official journal of the Society for NeuroscienceAugust 19, 2020Garza Carbajal A, Bavencoffe A, Walters ET, Dessauer CW.Primary antibodies are as follows: rabbit monoclonal anti-phospho RII (S99, 1:1000, clone 151, Abcam, #ab32390), mouse monoclonal anti phospho-p44/42 mitogen-activated protein J. Neurosci., August 19, 2020 • 40(34):6522–6535

(19) A(2A) R inhibition in alleviating spatial recognition memory impairment after TBI is associated with improvement in autophagic flux in RSCJournal of cellular and molecular medicineJune 1, 2020Zeng XJ, Li P, Ning YL, Zhao Y, Peng Y, Yang N, Xu YW, Chen JF, Zhou YG.protein 1 (LAMP1) (1:2000, Abcam, ab24170), anti‐phosphorylated protein kinase A (p‐PKA) (1:5000, Abcam, ab32390), anti‐PKA (1:2000, Abcam, ab75993), anti‐phosphorylated extracellular signal‐regulated kinase 2 (p‐ERK2)

(20) Norepinephrine Inhibits the Proliferation of Human Bone Marrow-Derived Mesenchymal Stem Cells via β2-Adrenoceptor-Mediated ERK1/2 and PKA PhosphorylationInternational Journal of Molecular SciencesMay 30, 2020Jessica Hedderich, Karima El Bagdadi, Peter Angele, Susanne Grässel, Andrea Meurer, Rainer H. Straub, Frank Zaucke, Zsuzsa Jenei-LanzlY204)/rabbit/mAb/Cell Signaling Technology®® /1:2500 ab32514, PKA R2, (Y116), 0,15 mg/mL/rabbit/mAb/Abcam/ 1:5000 ab32390, PKA R2 (E151) (phospho S99), 0,351 mg/mL/rabbit/ mAb/Abcam/1:2500 GAPDH Loading control antibody (GA1R)/mouse/mAb/

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Anti-PKA R2/PKR2 (phospho S99) antibody [E151] from Abcam

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Anti-PKA R2/PKR2 (phospho S99) antibody [E151] from Abcam

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