Western blot was performed using Anti-CD10 Rabbit Polyclonal Antibody (Product # PA5-29354) and a 100 kDa band corresponding to CD10 was observed in cell lines and tissues tested except for PC-3, Mouse Heart and Mouse Skeletal Muscle. Membrane enriched extracts (30 µg lysate) of LNCaP (Lane 1), PC-3 (Lane 2), Raji (Lane 3), Ramos (Lane 4), Mouse Kidney (Lane 5), Mouse Lungs (Lane 6), Mouse Heart (Lane 7), Mouse Skeletal Muscle (Lane 8) and Rat Kidney (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band (*) of 60 kDa was observed across cell lines and tissues.