Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human thyroid tissue stained using a ARRB2 polyclonal antibody (Product # PA5-23296) at 5 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Western blot was performed using Anti-beta-Arrestin 2 Polyclonal Antibody (Product # PA5-23296) and a 42 kDa band corresponding to beta-Arrestin 2 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of U-937 (Lane 1), HEL 92.1.7 (Lane 2), A549 (Lane 3), HEK-293 (Lane 4), MOLT-4 (Lane 5), SH-SY5Y (Lane 6), U-87 MG (Lane 7), Neuro-2a (Lane 8), HL-60 (Lane 9) and tissue extract (30 µg lysate) of Mouse Brain (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..