Immunofluorescent analysis of MEF2A in paraformaldehyde-fixed HeLa cells using a MEF2A polyclonal antibody (Product # PA5-27344) at a 1:200 dilution.

Western blot was performed using Anti-MEF2A Rabbit Polyclonal Antibody (Product # PA5-27344) and 47-76 kDa bands corresponding to canonical as well as SUMOylated MEF2A isoforms were observed in tissues tested except Mouse Liver which is reported to be negative. Uncharacterized bands (*) were observed at around 38 kDa and 200 kDa. Whole cell extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal muscle (Lane 2), Mouse Heart (Lane 3) and Mouse Liver (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..