Western blot was performed using Anti- PCK2 Polyclonal Antibody (Product # PA5-28078) and a band around 78 kDa corresponding to PCK2 was observed in cell lines and tissues tested. Membrane enriched extracts (30 µg lysate) of A-549 (Lane 1), Neuro-2A (Lane 2), HepG2 (Lane 3), HCT 116 (Lane 4), PC-12 (Lane 5), HEK-293 (Lane 6), HeLa (Lane 7) and Mouse Small Intestine (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).