Western blot analysis was performed on whole cell extracts (30 µg lysate) of LNCaP (Lane 1), PC-3 (Lane 2), DU 145 (Lane 3), MCF7 (Lane 4), A-431 (Lane 5), HEK 293 (Lane 6), tissue extracts of Mouse Liver (Lane 7) and Rat Liver (Lane 8). The blot was probed with Anti- FAAH Polyclonal Antibody (Product # PA5-32183, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 60 kDa band corresponding to FAAH was detected across cell lines tested except PC-3 and DU 145 which are reported to be negative for FAAH expression in comparison to LNCaP cell line.