RAD51 Antibody PA5-27195 from Thermo Fisher Scientific

Product Specs
ItemRAD51 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-27195
Quantity100 µL
ApplicationsImmunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate/TagUnconjugated
FormatPBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
ReactivityHuman, Mouse, Rat, Zebrafish
ClonalityPolyclonal
TargetRAD51
IsotypeIgG
HostRabbit
NCBI Full Gene NameRAD51 recombinase
NCBI Gene Aliaseszgc:77754, Reca, HsT16930, FANCR, BRCC5, RECA, HRAD51, RGD1563603, Rad51a, MRMV2, wu:fb38e12, RAD51A, HsRad51
Concentration0.14 mg/mL
ImmunogenRecombinant protein encompassing a sequence within the center region of human Rad51. The exact sequence is proprietary
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles

Add to Compare List

Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Short-Term Exposure to a 50 Hz Extremely Low-Frequency Electromagnetic Field (ELF-EMF) Leads to ROS-Mediated DNA Damage in Gynecological and Urological Cancer Cells In VitroInternational Journal of Molecular SciencesFebruary 7, 2026Gabriela Betlej, Ewelina Bator, Aleksandra Kwiatkowska, Maria Romerowicz-Misielak, Anna Koziorowska, Marek Koziorowski, Iwona RzeszutekSAB5700155), NSUN2 (1:100, BT-AP11581), METTL3 (1:50, RA20420), APE1 (1:100, BT-AP07734), RAD51 (1:100, PA5-27195), HuR (1:100, #07-1735), HO-2 (1:100, PA5-28334), XRCC1 (1:50, MA5-13412), VIM (1:250, MA5-11883), HSP90

(2) HAT1 functions as a lactyltransferase and mediates RPA1 lactylation to promote DNA repair and radioresistance in lung adenocarcinomaCell Death & DiseaseNovember 21, 2025Jiang He, Tangmin Lai, Yuzu Zhao, Zhiying Zhou, Liu Zhou, Dan Tao, Haonan Yang, Nan Li, Yu He, Shuheng Yang, et al.from PTM BIO. γ-H2AX (#9718) and RPA1 (#2267) were purchased from Cell Signaling Technology. RAD51 (#PA5-27195) was purchased from Thermo Fisher. Ki67(#HA721115) was obtained from HUABIO. For co-Immunoprecipitation,

(3) Melatonin suppresses cancer cell proliferation, DNA repair and expression of the oncogene TRIP13Cell Death DiscovOctober 27, 2025Wenqing Liu, Ans M. M. van Pelt, Geert HamerMTNR1A (1:1000, sc390328, Santa Cruz Biotechnology), MTNR1B (1:1000, ab203346, Abcam), RAD51 (1:1000; PA5-27195, Thermo Fisher Scientific), XRCC5 (1:1000, ab2172-500, Abcam). Secondary antibodies were IRDye 800CW

(4) UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in miceiScienceAugust 15, 2025Xiaochen Yu, Jie Cen, Yaxuan Zhang, Tongtong Li, Mingyu Zhang, Fei Gao, Hongbin Liu, Yongzhi Cao[EPR2877Y],Abcam,Cat#ab76420; RRID: AB_1524336 RAD51 Polyclonal Antibody,Thermo Fisher Scientific,Cat#PA5-27195; RRID: AB_2544671 MSH4 antibody,Abcam,Cat#ab58666; RRID: AB_881394 RNF212 antibody,"gifted from Dr.

(5) MCM9 deficiency impairs DNA damage repair during spermatogenesis, leading to Sertoli cell-only syndrome in humansCell Death DiscovJuly 1, 2025Xuan Sha, Xin Zhang, Hao Geng, Yuqian Li, Xun Xia, Guotong Li, Rong Hua, Kuokuo Li, Yang Gao, Qunshan Shen, et al.ab191914, ab235335, ab76420, all from Abcam), anti-RAD51, and anti-beta-tubulin (both 1:2000 dilution, PA5-27195, MA5-16308, both from Thermo Fisher Scientific). After three washes with TBST, membranes were incubated

(6) Targeting Replication Fork Processing Synergizes with PARP Inhibition to Potentiate Lethality in Homologous Recombination Proficient Ovarian CancersAdvanced science (Weinheim, Baden-Wurttemberg, Germany)May 1, 2025Pai Bellare G, Kundu K, Dey P, Philip KT, Chauhan N, Sharma M, Rajput SK, Patro BS.Imaging‐Alexa Fluor 488 dye and 594 (# C10337 and C10339), PCNA (#13‐3900, RRID AB_2533016), RAD51 (#PA527195, RRID: AB_2544671) antibody was from Invitrogen, Thermo Fisher Scientific (Waltham, MA, USA). MRE11

(7) Testis‐Specific PDHA2 Is Required for Proper Meiotic Recombination and Chromosome Organisation During SpermatogenesisCell ProliferationFebruary 20, 2025Guoqiang Wang, Kailun Fang, Yongliang Shang, Xu Zhou, Qiqi Shao, Si Li, Ping Wang, Charlie Degui Chen, Liangran Zhang, Shunxin Wangrabbit anti‐RPA2 (IF, 1:200; Abcam #ab76420), rabbit anti‐RAD51 (IF, 1:200; Thermo Fisher Scientific #PA5‐27195), rabbit anti‐DMC1 (IF, 1:200; Proteintech #13714‐1‐AP50), mouse anti‐phospho‐histone H2A.X (Ser139)

(8) Particulate hexavalent chromium exposure induces DNA double-strand breaks and inhibits homologous recombination repair in rat and human lung tissuesChemosphereFebruary 1, 2025Haiyan Lu, Sandra S. Wise, Jennifer H. Toyoda, Rachel M. Speer, Tayler J. Croom-Perez, Idoia Meaza, J. Calvin Kouokam, Jamie Young Wise, Gary Hoyle, Ning Chen, et al.2577S; 1:750) and anti-cytokeratin (Sigma-Aldrich, # C2931; 1:100), or anti-RAD51 (Thermo Fisher, # PA5-27195; 1:10000) and anti-PMCA ATPase (Invitrogen, # MA3-914; 1:500) diluted with TrueBlack background suppressor

(9) Inhibition of GSK3β is synthetic lethal with FHIT loss in lung cancer by blocking homologous recombination repairExperimental & molecular medicineFebruary 1, 2025Tao S, Pu Y, Yang EJ, Ren G, Shi C, Chen LJ, Chen L, Shim JS.(AF1522, Beyotime), PARP (9532 s, CST), cleaved-caspase3 (9664 s, CST), γ-H2A. X (9718 s, CST), RAD51 (PA5-27195, Invitrogen), BRCA1 (sc-6954, Santa Cruz), p-BRCA1 (s1423) (AB600, Beyotime), HSP90 (37-9400, Thermo Fisher

(10) USP1 deubiquitinates PARP1 to regulate its trapping and PARylation activityScience advancesNovember 15, 2024Nespolo A, Stefenatti L, Pellarin I, Gambelli A, Rampioni Vinciguerra GL, Karimbayli J, Barozzi S, Orsenigo F, Spizzo R, Nicoloso MS, et al.

(11) Matrine alkaloids modulating DNA damage repair in chemoresistant non-small cell lung cancer cellsBMC cancerOctober 16, 2024Wang F, Liu J, Liao W, Zheng L, Qian S, Mao L.antibodies, including Ki67 antibody (MA5-14520, dilution 1:200, Thermo Fisher, USA), RAD51 antibody (PA5-27195, 1:200, Thermo Fisher, USA), or POLD2 antibody (PA5-55401, 1:200, Thermo Fisher, USA), were used for

(12) Defining the KRAS- and ERK-dependent transcriptome in KRAS-mutant cancersScienceJune 7, 2024Jeffrey A Klomp, Jennifer E Klomp, Clint A Stalnecker, Kirsten L Bryant, A Cole Edwards, Kristina Drizyte-Miller, Priya S Hibshman, J Nathaniel Diehl, Ye S Lee, Alexis J Morales, et al.[RRID:AB_477629]; KRAS (3B10-2F2), cat. no. WH0003845M1 [RRID:AB_1842235]. From Invitrogen: RAD51, cat. no. PA5-27195 [RRID:AB_2544671]; SKA1, cat. no. PA5-61019 [RRID:AB_2647324], and NUF2, cat. no. MA5-31536 [RRID:AB_2787164].

(13) ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progressionCell proliferationApril 1, 2024Zhou X, Fang K, Liu Y, Li W, Tan Y, Zhang J, Yu X, Wang G, Zhang Y, Shang Y, et al.SC‐6248), rabbit anti‐RPA2 (1:200; Abcam, ab76420), rabbit anti‐RAD51 (1:200; Thermo Fisher Scientific, PA5‐27195), rabbit anti‐DMC1 (1:200; Proteintech, 13714‐1‐AP50), rabbit anti‐TEX11 (1:200), rabbit anti‐MSH4 (1:200;

(14) Regulation of meiotic telomere dynamics through membrane fluidity promoted by AdipoR2-ELOVL2Nat CommunMarch 14, 2024Jingjing Zhang, Mario Ruiz, Per-Olof Bergh, Marcus Henricsson, Nena Stojanović, Ranjan Devkota, Marius Henn, Mohammad Bohlooly-Y, Abrahan Hernández-Hernández, Manfred Alsheimer, et al.GR3184119-1), γH2AX (1:3000 for IF, Abcam; Ab11174, GR294890-8), RAD51 (1:500 for IF, Thermo Fisher Scientific; PA5-27195, UI2840658J), ELOVL2 (1:1000 for WB, Abcam; Ab176327, GR139744-4), CerS3(1:1000 for WB, 1:100 for IF)51,

(15) Caseinolytic mitochondrial matrix peptidase X is essential for homologous chromosome synapsis and recombination during meiosis of male mouse germ cellsAsian journal of andrologyMarch 1, 2024Feng HW, Zhao Y, Gao YL, Liu DT, Huo LJ.anti-ubiquitin antibody Lys48-specific clone Apu2-07 (1:100, #051307, Millipore), rabbit anti-RAD51 (1:200, PA5-27195, Invitrogen, Carlsbad, MA, USA), rabbit anti-HORMA domain-containing protein 1 (HORMAD1; 1:400, 13917-1-AP,

(16) Germline specific genes increase DNA double-strand break repair and radioresistance in lung adenocarcinoma cellsCell death & diseaseJanuary 12, 2024Liu W, Bruggeman JW, Lei Q, van Pelt AMM, Koster J, Hamer G.by the addition of primary antibodies against γ-H2AX (1:10.000, 05-636, Millipore) or RAD51 (1:50, PA5-27195, Thermo Fisher Scientific), or isotype immunoglobulin G in the case of negative controls. After overnight

(17) TLK1-mediated RAD54 phosphorylation spatio-temporally regulates Homologous Recombination RepairNucleic acids researchSeptember 8, 2023Ghosh I, Kwon Y, Shabestari AB, Chikhale R, Chen J, Wiese C, Sung P, De Benedetti A.protein from cell lysates were loaded in 8% SDS-PAGE gel and immunoblotted with RAD51 antibody (1:1000; PA5-27195, Thermofisher Scientific) diluted in 1% BSA + TBS + 0.1% Tween 20, RAD54 antibody (1:1000; sc-374598;

(18) SPIDR is required for homologous recombination during mammalian meiosisNucleic Acids ResearchMay 8, 2023Tao Huang, Xinyue Wu, Shiyu Wang, Ziyou Bao, Yanling Wan, Ziqi Wang, Mengjing Li, Xiaochen Yu, Yue Lv, Zhaojian Liu, et al.anti-RPA2 (1:200 dilution; Abcam #ab76420), rabbit anti-RAD51 (1:200 dilution; Thermo Fisher Scientific #PA5-27195), rabbit anti-DMC1 (1:200 dilution; homemade); mouse anti-γH2AX (pSer139) (1:1000 dilution; Millipore

(19) DNA repair protein FANCD2 has both ubiquitination-dependent and ubiquitination-independent functions during germ cell developmentThe Journal of biological chemistryMarch 1, 2023Zhao S, Huang C, Yang Y, Xu W, Yu Y, Wen C, Cao L, Gao F, Qin Y, Chen ZJ, et al.1:500 dilution), mouse anti-γH2AX (Millipore 05-636, 1:500 dilution), rabbit anti-RAD51 (Invitrogen PA5-27195, 1:100 dilution), mouse anti-MLH1 (BD Biosciences 550838, 1:50 dilution), rabbit anti-H3K4me2 (Millipore

(20) RBM46 is essential for gametogenesis and functions in post-transcriptional roles affecting meiotic cohesin subunitsProtein & cellJanuary 1, 2023Lv Y, Lu G, Cai Y, Su R, Liang L, Wang X, Mu W, He X, Huang T, Ma J, et al.Abclonal, A18850), rabbit anti-RAD51 polyclonal antibody (1:200 dilution; Thermo Fisher Scientific, PA5-27195) Primary antibody incubated overnight at four degrees in the refrigerator and were detected with Alexa

Be the First to Write a Review!

ICC/IF

ICC/IF

RAD51 Antibody

RAD51 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

Citations (39)

Reviews

RAD51 Antibody from Thermo Fisher Scientific

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map