Immunohistochemical analysis of CD68 using anti-CD68 Polyclonal Antibody (Product # PA5-32330) in Tonsil Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.

Western blot was performed using Anti-CD68 Polyclonal Antibody (Product # PA5-32330) and an 80 & 130 kDa band corresponding to Cd68 was observed in THP-1 and K-562 cells but not in Jurkat and Raji. Whole cell extracts (60 µg lysate, without adding DDT and boiling) of THP-1 (Lane 1), K-562 (Lane 2), Jurkat (Lane 3) and Raji (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).