Nogo Receptor Antibody PA5-28639 from Thermo Fisher Scientific

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Western blot was performed using Anti-Nogo Receptor Polyclonal Antibody (Product # PA5-28639) and 80 kDa band corresponding to Nogo receptor was observed along with uncharacterized bands (*) across cell lines and tissues tested except Mouse Skeletal Muscle, Mouse and Rat Heart. Tissue extracts of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse cerebellum (Lane 3), Rat cerebellum (Lane 4), Mouse skeletal Muscle (Lane 5), Mouse Heart (Lane 6), Rat Heart (Lane 7), whole cell extracts (30 µg lysate) of Hep G2 (Lane 8), IMR-32 (Lane 9) and SH-SY5Y (Lane 10) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Relative expression of Nogo receptor was observed in Mouse Brain, Rat Brain, Mouse cerebellum, and Rat cerebellum in higher levels in comparison to low levels in Mouse Skeletal Muscle, Mouse Heart, and Rat heart using Anti-Nogo receptor Polyclonal Antibody (Product # PA5-28639) in Western Blot. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with primary antibody (1:1000 Dilution) and detected by chemiluminescence Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
ICC/IF

Immunofluorescence analysis of Nogo Receptor was performed using SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PRKCI Rabbit Polyclonal Antibody (Product # PA5-28639) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing membrane staining of Nogo receptor in SH-SY5Y cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Nogo Receptor Antibody

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Thermo Fisher Scientific for
Nogo Receptor Antibody

The Nogo Receptor Antibody from Thermo Fisher Scientific is a Rabbit Polyclonal antibody to Reticulon 4 receptor, and rtn4r. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Nogo Receptor Antibody has been shown to work in the following applications: Immunocytochemistry, and Western Blot.

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Nogo Receptor Antibody

Nogo Receptor Antibody from Thermo Fisher Scientific

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Nogo Receptor Antibody from Thermo Fisher Scientific

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