ChAT Antibody PA5-29653 from Thermo Fisher Scientific

Product Specs
ItemChAT Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-29653
ApplicationsWB, ICC, IHC-fr, IHC-p
ReactivityHu, Ms, Rt
Concentration0.15 mg/mL
ImmunogenRecombinant protein encompassing a sequence within the N-terminus region of human Choline Acetyltransferase. The exact sequence is proprietary
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles
NCBI Gene AliasesCMS1A, CHOACTASE, CHOACTase, CMS6, B230380D24Rik, CMS1A2
NCBI Full Gene Namecholine O-acetyltransferase, choline acetyltransferase
HostRabbit
IsotypeIgG
TargetChAT
ClonalityPolyclonal
FormatPBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
Conjugate/TagUnconjugated
Quantity100 µL

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(1) Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell TypesBio ProtocJune 20, 2026Melanie K. Becher, Kaela Wilson, Italo Mocchettinumber: MA5-35259)" Acetylcholine,"Choline O-acetyltransferase (ChAT) (Invitrogen, catalog number: PA5-29653)","Acetylcholinesterase (AChE) (Invitrogen, catalog number: MA3-042)","Vesicular acetylcholine transporter

(2) Stromal and Neuronal Sources of Slit2/3 Ligands in the Adult Pancreas Exhibit Distinct Expression Patterns Independent of Robo2 Receptor Expression in the IsletbioRxivMay 21, 2026Matthew R. Wagner, Nicolas G. Pintozzi, Bjorn M. Schoff, Marissa I. Gold, Rachel H. Kasper, Nina G. Steele, Barak Blum802001); rabbit anti-NeuN, 1:100 (Cell Signaling Technology, 24307); rabbit anti-ChAT, 1:25 (Invitrogen, PA5–29653). Secondary antibodies used for immunostaining were AlexaFluor 488 (anti-rabbit, anti-rat, and anti-guinea pig

(3) The impact of seizures on REM sleep and the cholinergic pedunculopontine nucleus in a mouse model of Dravet SyndromebioRxivFebruary 21, 2026Krystal M. Santiago-Colon, Chandni Rana, Brandon Toth, Julia A. Kravchenko, Joseph Barden, Isabella VanHorn, Alexandra Jack, Alexander Hollier, Rida Qureshi, Hudson Haberland, et al.PBS) containing the primary antibody, rabbit polyclonal ChAT (1:1000; Thermo Fisher Scientific, Cat# PA5–29653, RRID:AB_2547128) for 48 hours at room temperature. The following primary antibodies were incubated

(4) Domain-like patterning of dendritic asymmetry and orientation defined by gap junctions in the mouse retinaiScienceDecember 19, 2025Gergely Szarka, Anett Futácsi, Tamás Kovács-Öller, Boglárka Balogh, Boldizsár Czéh, Béla Völgyi4,Rabbit,Alomone,APC-052,2000× an S-opsin,Rabbit,ThermoFisher,ABN1660,2000× a ChAT -Choline Acetyltransferase,Rabbit,ThermoFisher,PA5-29653,1500× a ChAT -Choline Acetyltransferase,Goat,ThermoFisher,PA1-4710,1000× a SMI32 –Non-phosphorylated

(5) Dorsolateral striatal acetylcholine reorganizes neural ensembles to anticipate threatbioRxivDecember 12, 2025Oyku Dinckol, Noah Harris Wenger, Charlie Maddox, Taylor Good, Aryanna Copling, Bhumi Pradipkumar Patel, F. Zehra Bozdag, Munir Gunes Kutlu1:1000 in 5% BSA) for ChrimsonR-tdTomato and an anti-ChAT antibody (rabbit anti-ChAT, Thermo Fisher #PA529653; 1:1000 in 5% BSA) for ChAT. Sections were then incubated with secondary antibodies (for mCherry ab:

(6) Phase synchrony between prefrontal noradrenergic and cholinergic signals indexes inhibitory controlNat CommunAugust 6, 2025Yuxiang Andy Liu, Yuhan Nong, Jiesi Feng, Guochuan Li, Paul Sajda, Yulong Li, Qi Wang(EPR20385, Abcam #ab209487) for Dbh detection and 1:300 rabbit anti-choline acetyltransferase (ChAT) (PA5-29653, ThermoFisher) for ChAT detection. On the next day, slices were washed 3× in PBS + Tween (0.0005%) solution

(7) Divalent metal ions enhance bone regeneration through modulation of nervous systems and metabolic pathwaysBioactive MaterialsMay 1, 2025Ying Luo, Baoyi Liu, Yashi Qiu, Lichen Li, Fan Yang, Chao Zhang, Jiali Wangconfocal microscope (Nikon AX). The following primary and secondary antibodies were utilized: ChAT (PA5-29653, Thermo Fisher Scientific), TH (AB152, Sigma-Aldrich), CGRP (14959S, Cell Signaling Technology), CD31

(8) Gap junctions fine-tune ganglion cell signals to equalize response kinetics within a given electrically coupled arrayiScienceJune 21, 2024Szarka G, Ganczer A, Balogh M, Tengölics ÁJ, Futácsi A, Kenyon G, Pan F, Kovács-Öller T, Völgyi B.the same protocol as the serotonin labeling for rabbit choline acetyltransferase (ChAT; ThermoFisher, PA5-29653, 1,500x) and rabbit hyperpolarization-activated cyclic nucleotide-gated potassium channel 4 (HCN4; Alomone Labs

(9) Comparative In Vivo Imaging of Retinal Structures in Tree Shrews, Humans, and MiceeNeuroMarch 1, 2024Grannonico M, Miller DA, Liu M, Krause MA, Savier E, Erisir A, Netland PA, Cang J, Zhang HF, Liu X.Cat#14-1441-82,AB_842767,Rat,1:200,N/Y aChAT,Choline acetyltransferase,Thermo Fisher Scientific catalog #PA5-29653,AB_2547128,Rabbit,1:500,Y/Y aGAD67,Glutamate decarboxylase 67,Millipore catalog #MAB5406,AB_2278725,Mouse,1:250,Y/Y aGFAP,Glial

(10) Characterization of neural damage and neuroinflammation in Pax6 small-eye miceExperimental Eye ResearchJanuary 1, 2024James D. Cole, John A. McDaniel, Joelle Nilak, Ashley Ban, Carlos Rodriguez, Zuhaad Hameed, Marta Grannonico, Peter A. Netland, Hu Yang, Ignacio Provencio, et al.AB_2923082, Waltham, MA), anti-choline acetyltransferase (ChAT,1:250; Thermo Fisher Scientific Cat# PA5–29653, RRID: AB_2547128, Waltham, MA), anti-AP-2 (1:200, DSHB Cat# 5E4, RRID: AB_2056333, Iowa City, IA) anti-glial

(11) Serotonin is a gap junction-permeable neuronal tracer in the mouse retinaFront Ophthalmol (Lausanne)January 1, 2023Szarka G, Hoffmann G, Kovács-Öller T, Völgyi B.the same protocol as the serotonin labeling for rabbit choline acetyltransferase (ChAT; ThermoFisher, PA5-29653, 1,500×) and rabbit hyperpolarization-activated cyclic nucleotide-gated potassium channel 4 (HCN4; Alomone Labs

(12) Deciphering the dynamic niches and regeneration-associated transcriptional program of motoneurons following peripheral nerve injuryiScienceSeptember 16, 2022Zhang Y, Xu L, Li X, Chen Z, Chen J, Zhang T, Gu X, Yang J.mouse anti-ATF3 antibody (1:100 dilution, ab58668, Abcam), rabbit anti-ChAT antibody (1:250 dilution, PA5-29653, Invitrogen), and mouse anti-NeuN antibody (1:250 dilution, MAB377, Sigma) and Hoechst 33342 (1:3000

(13) Targeting the tamoxifen receptor within sodium channels to block osteoarthritic painCell reportsAugust 23, 2022McCollum MM, Larmore M, Ishihara S, Ng LCT, Kimura LF, Guadarrama E, Ta MC, Vien TN, Frost GB, Scheidt KA, et al.(Proteintech, 26975-1-AP); RFP-Booster 1:2000x (Chromotek, rb2AF568-50); anti-ChAT antibody 1:500x (Invitrogen, PA5-29653); DAPI (Invitrogen, D1306) and cell membrane stain 1:1000x (Invitrogen, C10607). The images were obtained

(14) Focal impaired awareness seizures in a rodent model: A functional anatomyEpilepsia openMarch 1, 2022Adotevi N, Kapur J.following antibodies: mouse anti‐NeuN (1:500; Millipore, MAB377), rabbit anti‐ChAT (1:500, Invitrogen, PA5‐29653), Alexa Fluor 488 goat anti‐mouse (1:500; Invitrogen, A11029), and Alexa Fluor 488 goat anti‐rabbit

(15) Pegylated Insulin-Like Growth Factor 1 attenuates Hair Cell Loss and promotes Presynaptic Maintenance of Medial Olivocochlear Cholinergic Fibers in the Cochlea of the Progressive Motor Neuropathy MouseFrontiers in neurologyJanuary 1, 2022Bieniussa L, Kahraman B, Skornicka J, Schulte A, Voelker J, Jablonka S, Hagen R, Rak K.NHS, 1% BSA, 0.3% TritonX100, 0.1% Tween20) containing primary antibodies against ChAT (anti-rabbit, PA529653, Invitrogen, 1:1000) and ßIII-tubulin (anti-mouse, MAB1192, R&D System, Tuj1, 1:1000) for 3 h at room

(16) Motor neuron preservation and decrease of in vivo TDP-43 phosphorylation by protein CK-1δ kinase inhibitor treatmentScientific reportsMarch 10, 2020Martínez-González L, Rodríguez-Cueto C, Cabezudo D, Bartolomé F, Andrés-Benito P, Ferrer I, Gil C, Martín-Requero Á, Fernández-Ruiz J, Martínez A, et al.anti-Iba-1 (019-19741) was purchased in Wako Chemicals (Richmond, VI, USA), and anti-ChAT antibody (PA5-29653) was obtained from Life Technologies (Carlsbad CA, US). Dilutions are shown in Table 1. Animal procedures Prp-hTDP-43(A315T)

(17) SnRNA-seq reveals the heterogeneity of spinal ventral horn and mechanism of motor neuron axon regenerationiScienceJanuary 1, 0001"Zhu Y, Luan C, Gong L, Gu Y, Wang X, Sun H, Chen Z, Zhou Q, Liu C, Shan Q, et al."RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, ChAT Polyclonal Antibody,Invitrogen,Cat# PA5-29653; RRID: AB_2547128 Cacna2d2 Polyclonal Antibody,Invitrogen,Cat# PA5-95536; RRID: AB_2807338 Anti-Mouse

(18) Angiopoietin-1 Knockout Mice as a Genetic Model of Open-Angle GlaucomaTranslational vision science & technologyJanuary 1, 0001"Thomson BR, Grannonico M, Liu F, Liu M, Mendapara P, Xu Y, Liu X, Quaggin SE."anti-calbindin (Chemicon, #AB2724, 1:1000; Sigma-Aldrich), rabbit anti-choline acetyltransferase (#PA5-29653, 1:500; ThermoFisher Scientific), goat anti-PROX1 (#AF2727, 1:500; R&D Systems, Minneapolis, MN, USA),

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ChAT Antibody from Thermo Fisher Scientific

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ChAT Antibody from Thermo Fisher Scientific

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