Western blot analysis was performed on whole cell extract (30 µg lysate) of SKOV3 (Lane 1), MDA-MB-231 (Lane 2), MCF7 (Lane 3), GTL16 (Lane 4), HCT116 (Lane 5), PC3 (Lane 6), A431 (Lane 7), PANC-1 (Lane 8), and A549 (Lane 9). The blot was probed with Anti-VCP Polyclonal Antibody (Product # PA5-29638, 1:8,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 97 kDa band corresponding to VCP was observed in all cell lines.