Immunofluorescence analysis of CTGF was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with CTGF Polyclonal Antibody (Product # PA5-32193) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization. Panel (a-d) shows representative HUVEC control cell, whereas Panel (e-h) represent HUVEC cells treated with Protein Transport Inhibitor Cocktail (500X), 1X, 4 hours. Similarly, panel (i-l) represent HEK 293 control cells and Panel (m-p) represent HEK 293 treated with Protein Transport Inhibitor Cocktail (500X), 1X, 4 hours. The images were captured at 60X magnification.