Estrogen Receptor alpha (D8H8) Rabbit mAb 8644S from Cell Signaling Technology

Product Specs
ItemEstrogen Receptor alpha (D8H8) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number8644S
Quantity100 µl
ApplicationsW, W-S, IP, ChIP, ChIP-seq, C&R
ReactivityH
TargetER-alpha
Molecular Weight66
ClonalityMonoclonal
HostRabbit
NCBI Full Gene Nameestrogen receptor 1
NCBI Gene AliasesER, ESR, ESRA, ESTRR, Era, NR3A1
CloneD8H8
Research AreaNuclear Receptor Signaling

Questions and Answers

Can this antibody be used for studying estrogen receptor signaling in breast cancer?

Yes, the Estrogen Receptor alpha (D8H8) Rabbit mAb has been utilized in various studies related to breast cancer. For instance, it was employed in Western blot analyses to assess ERα expression in breast cancer cell lines, demonstrating its relevance in understanding estrogen receptor signaling pathways in this context ().

Is this antibody effective for immunoprecipitation experiments?

The Estrogen Receptor alpha (D8H8) Rabbit mAb has been shown to work effectively in immunoprecipitation experiments, as indicated by its use in studies examining the interactions and functions of estrogen receptors in various cellular contexts ().

What applications has this antibody been cited in?

This antibody has been cited in several applications, including Western blotting, immunoprecipitation, chromatin immunoprecipitation, and ChIP-seq, making it versatile for various experimental setups in cell biology and cancer research (, ).

Does this antibody have any documented interactions with other proteins in cancer research?

Yes, the Estrogen Receptor alpha (D8H8) Rabbit mAb has been referenced in studies that explore its interactions with other proteins, such as COL11A1, which is associated with tamoxifen resistance in breast cancer. This highlights the antibody's utility in investigating complex signaling networks in cancer biology ().

Cell Signaling Technology

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Phone: 877-616-CELL
Phone: 978-867-2388
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Prenatal bisphenol A exposure reprograms SF1-lactylation pathways to promote endometriosis susceptibilityiScienceMay 15, 2026Xiaohan Liu, Yanxia Fu, Liuxuan Huang, Donghan Li, Paul Yao, Liqin ZengRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, ERβ antibody,Cell Signaling Technology,Cat# 8644 SF1 antibody,Abcam,Cat# ab217317 Ki-67 antibody,Abcam,Cat# ab15580 γH2AX antibody,Cell Signaling Technology

(2) AdipoR1–AMPK axis suppresses breast cancer across molecular subtypes via multimodal cell death pathways, including ferroptosis and apoptosisCell Death & DiseaseMarch 26, 2026Shinya Sato, Takashi Yamanaka, Yukako Komori, Mutsumi Ishida, Yoshiyasu Nakamura, Toshinari Yamashita, Yohei Miyagi(Cell Signaling Technology, Danvers, MA, USA; 5831S, 2535S, 4970S, 3294S, 2895S, 43996S, 14823S, 10741S, 8644S, and 9664S, respectively). The blots were incubated with HRP-conjugated goat anti-rabbit antibody (Cytiva,

(3) Role of DCAF8 in Mammary Ductal Elongation and Branching MorphogenesisJournal of Mammary Gland Biology and NeoplasiaMarch 6, 2026Qianying Han, Miaomiao Ban, Ting Zhang, Tianyun Yu, Xingyue Yan, Yumeng Hu, Pengfei Wu, Yanfang Ma, Luzheng Xu, Yanrong Su, et al.(#sc-52950, Santa Cruz, 1:1000 dilution), anti-β-actin (#A1978, Sigma-Aldrich, 1:8000 dilution), anti-ERα (#8644S, Cell Signaling Technology, 1:1000 dilution), anti-PR (#8757S, Cell Signaling Technology, 1:1000 dilution).

(4) Structural and mechanistic analysis of covalent ligands targeting the RNA-binding protein NONOCell Chemical BiologyFebruary 19, 2026Garrett L. Lindsey, Thomas K. Hockley, Alejandro Villa Gomez, Andrew C. Marshall, William R. Brothers, Colin T. Finney, Jacob Gross, Archa H. Fox, Gene W. Yeo, Bruno Melillo, et al.RESOURCES,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Rabbit anti- ESR1 Receptor,Cell Signaling Tech,Cat#:8644S; RRID: AB_2617128 RXRA Rabbit Poly Ab,Proteintech,Cat#: 21218-1-AP; RRID: AB_10693633 Mouse anti-

(5) Squalene synthase induces ERα expression via cholesterol supplementation to confer statin resistance in lung cancer cellsCancer Cell InternationalFebruary 13, 2026Yi-Fang Yang, Yu-Chan Chang, Ming-Hsien Chan, Min-Hsi Lin, Chih-Jen Yang, Ming-Shyan Huang, Yi-Hua Jan, Michael Hsiaousing primary antibodies against SQS (1:2000, Genetex, GTX104091) and ERα (1:1000, Cell Signaling, 8644 s) with overnight incubation at 4 °C. Following washing and incubation with the appropriate horseradish

(6) Combined therapy with the estrogen receptor ERα and IFNα-2b suppresses HBV replication by inducing GBP1 expressionFrontiers in ImmunologyJanuary 16, 2026Yadi Li, Jiaojiao Gong, Guili Tan, Haiying Luo, Xiaoxia Hu, Bo Qinfixed in 4% polyethylene glycol (PEG) methanol. Anti-GBP1 (15303-1-AP, Prointech, China), anti-ERα (8644, CST, USA), and anti-FLAG-Tag (monoclonal mouse Flag, 66008-3, murine IgX, Proteintech) were diluted

(7) ERα activates NAMPT/IL-33 signaling to enhance beige thermogenesis and metabolic fitnessScience AdvancesJanuary 9, 2026Ruoci Hu, Jooman Park, Yanyu Qian, Shaolei Xiong, Ahmad Hamza Elsabbagh, Aditya Chhikara, Huailing Fan, Zuoxiao Shi, Lifeng Liu, Yanhui Li, et al.antibodies at RT for 2 hours. Antibodies used for Western blotting were rabbit anti-ERα (1:1000; no. 8644, Cell Signaling Technology), mouse anti-ERβ (1:500; GTX70174, GeneTex), rabbit anti-β actin (1:2000;

(8) CDK2 inhibition enhances CDK4/6 inhibitor antitumor activity in comprehensive breast cancer PDX model screenNPJ Breast CancerDecember 3, 2025Nealia C. House, Maxine M. Chen, Sima Khazaei, Victoria Brown, Philip Ramsden, David H. Peng, Sydney Moore, Liang Yuan, Rentian Wu, Fangyang Wang, et al.3541; RRID:AB_331497), pNCL1 1:500 (ab155977; RRID:AB_3075493), Estrogen Receptor 1 Alpha 1:1000 (CST-8644; RRID:AB_2617128), HER2 1:1000 (CST 2165; RRID:AB_10692490), HSP90 1:500 (SC-13119; RRID:AB_675659),

(9) Inhibition of TBK1/IKKε mediated RIPK1 phosphorylation sensitizes tumors to immune cell killingCell Death DiscoveryNovember 28, 2025Anastasia Piskopou, David W. Vredevoogd, Xiangjun Kong, Daniel S. Peeper, Maarten Altelaar, Kelly E. Steckerovernight at 4 °C with the following primary antibodies: RIPK1 (#3493, CST), TBK1 (#5483, CST), IKKε (#8644, CST), and β-actin (#4970, CST). Subsequently, membranes were incubated with an anti-rabbit horseradish

(10) Highly efficient and long-acting split-and-mix proteolysis targeting chimera based on self-assembled polylactic acidNature CommunicationsNovember 26, 2025Mei-miao Zhan, Hailing Chen, Meiling He, Chunli Song, Zijun Jiao, Na Liu, Zhihong Liu, Zhanfeng Hou, Ying Chen, Zhibo Song, et al.Image LabTM Software. Primary antibodies included BRD4 (1:1000; Abcam, ab128874), ERα (1:1000; CST, #8644), CDK4 (1:1000; CST, #12790), β-Tubulin (1:1000; CST, #15115) and GAPDH (1:5000; CST, #97166). For immunoprecipitation,

(11) Co-targeting CDK4/6 enhances anti-cancer activity and alleviates immune-related adverse events of anti-PD-1 antibody for breast cancerCell Reports MedicineNovember 18, 2025Shu-Wei Hu, Ming-Hsin Yeh, Yu-Hao He, Ya-Ling Wei, Fang-Ju Cheng, Yi-Lun Yeh, Thanh Kieu Huynh, Pei-Tong Liu, Dai-Wei Hu, Tzu-Hsuan Chang, et al.antibody,Sigma-aldrich,SAB4504178 Anti-Phospho-ERα antibody,Cell Signaling,#2511; RRID: AB_331289 Anti-ERα antibody,Cell Signaling,#8644; RRID: AB_2617128 ,, Biological samples,Biological samples,Biological samples ,, Human breast tumor

(12) An integrated computational, in vitro and metabolomics approach to the discovery of novel aromatase inhibitors with anti-breast cancer activitiesScientific ReportsNovember 17, 2025Arnatchai Maiuthed, Jaruwan Chatwichien, Nichawadee Sandech, Meng Chieh Yang, Sucheewin Krobthong, Pattarawit Rukthong, Jisnuson Svasti, Somsak Ruchirawat, Chatchakorn Eurtivong#89954) and ERα activation antibody sampler kit (cell signaling #49757) including ERα (D8H8) rabbit mAb (#8644), phospho-estrogen receptor α (Ser118) (16J4) mouse mAb (#2511), and phospho-ERα (Ser167) (D5W3Z) rabbit

(13) Integrative miRNOMe profiling reveals the miR ‐195‐5p– CHEK1 axis and its impact on luminal breast cancer outcomesMolecular OncologyNovember 1, 2025Veronika Boušková, Marie Ehrlichová, Alžběta Spálenková, Ivona Krus, Simona Šůsová, Viktor Hlaváč, Vlasta Němcová, Renata Koževnikovová, Markéta Trnková, David Vrána, et al.monoclonal antibody, #336445, R&D Systems, Minneapolis, MN, USA), ERα (1:000, rabbit monoclonal antibody, #8644, Cell Signaling, Danvers, Massachusetts, USA), cleaved caspase‐3 (1:500, rabbit monoclonal antibody,

(14) Targeting ESR1 restores SQSTM1-dependent autophagy and sensitizes ER-positive breast cancer to oxidative and radiation stressCell Death DiscovOctober 7, 2025Yi-Fang Yang, Zhao-Jing He, Han-Hsi Kuo, Yu-Yu Lin, Cheorl-Ho Kim, Huei-Yu Cai, Chi-Long Chen, Michael Hsiao, Ying-Chung Chen, Peter Mu-Hsin Chang, et al.Antibody,Catalog number,Company,Dilution Estrogen receptor α,8644S,Cell Signaling,1:1000 Phospho-ESR1 (Ser118),ab32396,Abcam,1:1000 Phospho-ESR1 (Ser106),ab75753,Abcam,1:1000 Phospho-ATM

(15) Preclinical Efficacy of the Estrogen Receptor Degrader Fulvestrant in Combination with RAF/MEK Clamp Avutometinib and FAK Inhibitor in a Low-Grade Serous Ovarian Cancer Animal Model with Acquired Resistance to Chemotherapy and Aromatase InhibitorInternational Journal of Molecular SciencesSeptember 13, 2025Cem Demirkiran, Stefania Bellone, Victoria M. Ettorre, Miranda Mansolf, Tobias Max Philipp Hartwich, Blair McNamara, Michelle Greenman, Yang Yang-Hartwich, Elena Ratner, Niccoló G. Santin, et al.topoisomerase 1 (TOP1) (BD Biosciences # 556597, Franklin Lakes, NJ, USA), and estrogen receptor alpha (D8H8) (#8644, Cell Signaling Technology). Blots were designed by using Clarity or Clarity Max Western ECL Blotting

(16) Estrogen-like Activity of Scrophularia buergeriana Root Extracts in MCF-7 CellsBiomedicinesSeptember 4, 2025Hye-Yeong Song, Jinsu Choi, Eunwoo Jeong, Harang Park, Juyeong Moon, Min-ah Kim, Javokhir Rustamov, Hwan-Soo Yoo, Tack-Joong Kimspecific primary antibodies (1:2500) that target ERα (Cell Signaling Technology, Danvers, MA, USA; #8644), p-AKT (Cell Signaling Technology, Danvers, MA, USA; #9271), AKT (Cell Signaling Technology, Danvers,

(17) HDAC7 influences ER-⍺ transcription via NCoR-HDAC3 dissociationBiochimica et Biophysica Acta (BBA) - Proteins and ProteomicsSeptember 1, 2025Ishadi K. M. Kodikara, Valentine O. Nwanelo, Angela K. Belanger, Mary Kay H. Pflum(Cell signaling, Cat. No. 12041T), RXR (Cell signaling, Cat. No. 5388S), ER-α (Cell signaling, Cat. No. 8644), and HDAC7 (Cell signaling, Cat. No. 10831S) primary antibodies, along with anti-rabbit IgG HRP-linked

(18) Harnessing artificial intelligence to identify Bufalin as a molecular glue degrader of estrogen receptor alphaNat CommunAugust 22, 2025Shilong Jiang, Keyi Liu, Ting Jiang, Hui Li, Xiao Wei, Xiaoya Wan, Changxin Zhong, Rong Gong, Zonglin Chen, Chan Zou, et al.Recombinant human PKC delta protein (ab60844) was purchased from Abcam (Cambridge, UK). Antibodies targeting ERα (Cat. no. 8644, WB, 1:1000), LC3 (Cat. no. 12741, WB,1:1000), PARP (Cat. no. 9532, WB,1:1000), and Bcl-2 (Cat. no.15071,

(19) JNK pathway suppression mediates insensitivity to combination endocrine therapy and CDK4/6 inhibition in ER+ breast cancerJ Exp Clin Cancer ResAugust 19, 2025Sarah Alexandrou, Christine S. Lee, Kristine J. Fernandez, Celine E. Wiharja, Leila Eshraghi, John Reeves, Daniel A. Reed, Neil Portman, Zoe Phan, Heloisa H. Milioli, et al.Cell Signalling Technology, detecting JNK2 protein), JNK1 (3708; Cell Signalling Technology), ERα (8644; Cell Signalling Technology), phospho-cJUNSer63 (sc-822; Santa Cruz Biotechnology), cJUN (9165; Cell Signalling

(20) Combined inhibition of KAT6A/B and Menin reverses estrogen receptor-driven gene expression programs in breast cancerCell Reports MedicineJuly 15, 2025Sarah Naomi Olsen, Bryn Anderson, Charlie Hatton, Zhengtao Chu, Christopher Simpkins, Yanhe Wen, Wallace Bourgeois, Elena L. Haarer, Myles Brown, Rinath Jeselsohn, et al.,Cell Signaling Technology,Cat#14623; RRID: AB_2798543 Rabbit anti-Estrogen Receptor alpha,Cell Signaling Technology,Cat#8644S; RRID: AB_2617128 Estrogen Receptor alpha Monoclonal Antibody (TE111.5D11),Invitrogen,Cat#MA112692;

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Estrogen Receptor alpha (D8H8) Rabbit mAb

Estrogen Receptor alpha (D8H8) Rabbit mAb from Cell Signaling Technology

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Estrogen Receptor alpha (D8H8) Rabbit mAb from Cell Signaling Technology

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