TNF-alpha (D2D4) XP ® Rabbit mAb 11948S from Cell Signaling Technology

Product Specs
ItemTNF-alpha (D2D4) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number11948S
Quantity100 µl
ApplicationsW, IP, IF-IC, FC-FP
ReactivityM
TargetTNF-alpha mouse
Molecular Weight17,25,28
ClonalityMonoclonal
HostRabbit
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesDIF, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnfa, Tnfsf1a, Tnlg1f
CloneD2D4
Research AreaImmunology and Inflammation

Questions and Answers

How has the TNF-α (D2D4) antibody been utilized in research related to neuronal excitability?

The TNF-α (D2D4) antibody has been shown to mitigate hyperexcitability in CA1 pyramidal neurons in a model of hyperinflammatory experimental cerebral malaria. In studies, treatment with this anti-TNF antibody reduced neuronal hyperexcitability and associated sickness behavior, indicating its potential role in modulating TNF-mediated neuroinflammation ().

Can this antibody be used effectively in studies involving macrophage polarization?

While specific studies using the TNF-α (D2D4) antibody in macrophage polarization were not detailed, TNF-α is a well-known cytokine involved in macrophage activation and polarization. Therefore, it is reasonable to consider that this antibody could be relevant in experiments investigating the effects of TNF-α on macrophage phenotypes, particularly in inflammatory contexts.

What evidence supports the use of this antibody in inflammation-related research?

The TNF-α (D2D4) antibody has been cited in numerous studies, including those examining the role of TNF-α in various inflammatory conditions. For instance, it has been implicated in the modulation of inflammatory responses in models of diseases such as atherosclerosis and periodontitis, where TNF-α plays a critical role in disease progression and immune response (, ).

Is there any indication of the antibody's effectiveness in vivo?

Yes, the TNF-α (D2D4) antibody has demonstrated effectiveness in vivo, particularly in studies where it was used to block TNF-α signaling, leading to reduced inflammation and improved outcomes in models of disease. For example, its application in experimental cerebral malaria showed a reduction in neuronal hyperexcitability and behavioral changes associated with the disease ().

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Glycosylated graphene-nylon fiber: A new material platform to balance the nanotoxicity and bioactivity for anti-infective therapyBioactive MaterialsJuly 1, 2026Gefei Li, Juan Mo, Jie Jing, Lucen Li, Yuhao Ju, Wenxiao Ma, Ziyao Zhuang, Xuanxuan Yu, Jie Deng, Jing Lin, et al.min. Sections were incubated in a humidifying box with the primary antibodies (rabbit anti-TNF-α, CST, 11948, 1:100; rabbit anti-IL-6, CST, 12912, 1:100; rabbit anti-CD31, CST, 15585, 1:100; mouse anti-α-SMA,

(2) Ganoderma lucidum mediates microglial polarization and ameliorates experimental autoimmune encephalomyelitis by reducing oxidative stress and inhibiting NF-κB/STAT3 pathwayChin MedApril 10, 2026Lu Zhang, Jie Chen, Sauchu Yuen, Meiling Wu, Wenting Li, Shenyu Yan, Jiangang ShenCell Signaling), COX2(sc1747, Santa Cruz), IL-6 (ab290735, Αbcam), IL-1β(sc-52012, Santa Cruz), TNF-α (11948 T, CST), Anti-mouse antibody (7076 s, Cell Signaling), Anti-rabbit antibody (7074 s, Cell Signaling),

(3) Ginsenoside Rb1 Ameliorates Age‐Related Cognitive Impairment Potentially by Modulating the NF‐κB Signaling PathwayBrain and BehaviorApril 1, 2026Wei‐Feng Lu, Yu‐Zhen Zhang, Zhi‐Gang Chen, Qun‐Lin Xiao, Shi‐Ye Ke1A; purity greater than 98%) was obtained from Victory (Sichuan, China). Antibodies against TNF‐α (11948S) and GAPDH (5174S), and an NF‐κB pathway sampler kit (9936) were purchased from Cell Signaling Technology

(4) Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathwayChin MedMarch 26, 2026Zhi-Xian Jiang, Xue-Liang Chen, Qi Sun, Li-Chao Yang, Ya-Wei Zhang, Qiang Wu, Heng-Chang Yao, Dan Zhang, Lian-Wen Yuan(DF6921, Affinity Bioscience), IL-1β [12242, Cell Signaling Technology (CST), Danvers, USA], TNF-α (11948, CST), IL-6 (CSB-PA06757A0RB, CUSABIO, Wuhan, China), p-STAT3 (AF3293, Affinity Bioscience), STAT3 (10253-2-AP,

(5) Dimercaprol Reprograms Intestinal Redox Homeostasis and Organelle Crosstalk to Combat Iron-Induced Gut Dysbiosis Through NRF2/HO-1 SignalingAntioxidants (Basel)March 11, 2026Asad Khan, Zongliang Xiong, Iftikhar Ali Khan, Xiangyu Cheng, Qihui Luo, Lanlan Jia, Wentao Liu, Chao Huang, Zhengli ChenChina)",A16288,WB Anti-IL-6,"Huabio (Hangzhou, China)",EM1701-45,"WB, IF" Anti-TNF-α,"Cell Signaling Technology (Danvers, MA, USA)",11948T,"WB, IHC" Anti-GRP78,"Proteintech (Wuhan, China)",11587-1-AP,"WB, IF" ATF4,"Huabio (Hangzhou, China)",ET1612-37,"WB, IF

(6) Effects of Fipronil Exposure on Glucose Metabolism Disorder via the Gut Microbiota and InflammationToxicsFebruary 27, 2026Ziquan Lv, Yuxuan Wu, Tingting Cao, Changfeng Peng, Xuan Zou, Xinyue Xu, Dan Wang, Ying Chen, Guangnan Liu, Yuebin Ke, et al.(9315S) anti-tubulin (3873S), anti-IL-6 (D5W4V) (12912S), anti-IL-1β (3A6) (12242S), anti-TNF-α (D2D4) (11948S), anti-NF-κB (C22B4) (66286SF) (all obtained from Cell Signaling Technology, Beverly, MA, USA); anti-Pepck

(7) Evaluation of Intratympanic Alpha-Lipoic Acid and Diltiazem as Alternatives to Dexamethasone in Noise-Induced Hearing Loss in a Murine ModelAntioxidants (Basel)February 21, 2026Jae Sang Han, Kyusun Park, Ye Lin Kim, Ji Hyung Lim, So Young Park, Shi Nae Parkwith primary antibodies against NF-κB (#8242, Cell Signaling Technology, Danvers, MA, USA), TNF-α (#11948S, Cell Signaling Technology, MA, USA), IL-6 (#ab290735, Abcam, Cambridge, UK), IL-1β (#AF-401-NA, R&D System

(8) Natural polysaccharide riclin acts as an immune adjuvant to enhance chemotherapy efficacy in NSCLCnpj Precision OncologyFebruary 4, 2026Yaqiong Miao, Xiaofen Liu, Jing Tao, Yang Jiao, Yizhuo Fan, Xiaqing Sun, Junhao Liu, Zhao Ding, Jianfa Zhang, Qin Gao, et al.(Beyotime) and quantified with ImageJ. Following antibodies were used: β-actin (3700, monoclonal), TNF-α (11948, monoclonal), IL-6 (12912s, monoclonal), and p-NF-κB p65 (3033T, monoclonal) from Cell Signaling Technology,

(9) Temporal Proteomic and Phosphoproteomic Profiling Deciphers Molecular Dynamics of Acute-to-Chronic Kidney Disease After Ischemia-Reperfusion Injury, With Dock2 Emerging as a Key RegulatorMolecular & Cellular ProteomicsFebruary 1, 2026Shaowu Zhang, Huasheng Luo, Miaotao Wei, Yanmei Yu, Hongluan Wu, Tongtong Ma, Minjie Zhang, Huafeng Liu, Peng Wang(Cell Signaling Technology, cat. 2029S, dilution 1:1000); anti-TNF-α (Cell Signaling Technology, cat. 11948S, dilution 1:1000); anti-Dock2 (Millipore, cat. 09–454, dilution 1:500); anti-IKKβ (Starter, cat. S0B1387,

(10) Piezo1-Targeted Magnetoacoustic Nanobubbles Rescue Alzheimer’s Pathology by Electromechanical Neuromodulation and Amyloid-β ClearanceACS NanoJanuary 20, 2026Fangxuan Chu, Xiaoqing Zhou, Ren Ma, Ruixu Liu, Xu Liu, Kai Zhu, Yuheng Wang, Xin Wang, Ying Li, Shunqi Zhang, et al.(ab32127, Abcam, 1:1000), IL-1β (ab254360, Abcam, 1:1000), NR-2B (ab65783, Abcam, 1:1000), TNF-α (#11948, CST, 1:1000), PSD-95 (ab238135, Abcam, 1:1000), IL-6 (#12912S, CST, 1:1000), IL-10 (ab189392, Abcam,

(11) Modulation of the NF-κB signaling pathway by the combined strategy of tocilizumab and dexamethasone for asthma therapyRespiratory ResearchJanuary 8, 2026Xinyi Li, Jingyi Zhang, Yihui Feng, Qihui Zhou, Chunling Zhang(TBST). The primary antibodies, including TNF-α rabbit monoclonal antibody (Cell Signaling Technology, #11948T), NF-κB rabbit-derived monoclonal antibody (Cell Signaling Technology, #8242), p-NF-κB rabbit-derived

(12) Antispasmodic and Anti-inflammatory Effects of Kratom Leaf Extract on Acetic Acid-induced Ulcerative Colitis in MiceIn VivoJanuary 2, 2026SAKDA PRADAB, CHUTHA TAKAHASHI YUPANQUI, CHITTIPONG TIPBUNJONG, FITTREE HAYEEAWAEMA, NARUMON SENGNON, JURAITHIP WUNGSINTAWEEKUL, PISSARED KHUITUANwas from Helena Laboratories (Beaumont, TX, USA). Rabbit anti-β-actin (#4976), rabbit anti-TNF-α (#11948), and rabbit anti-IL-1β (#31202) were purchased from Cell Signaling Technology (Danvers, MA, USA). Other

(13) Luteolin attenuates RA-associated chronic pain by targeting the LDHA/H3K9la/NFATC2 axis to suppress Th17 cell differentiation and central infiltrationJournal of Pharmaceutical AnalysisJanuary 1, 2026Yuepeng Jiang, Yang Zhao, Xiao Ma, Xiaoxuan Zhao, Mengjia Zheng, Junjun Wen, Cunrui Yuan, Xinyi Ding, Chengping Wenantibody (Cat. No.: 12912; Cell Signaling Technology, Inc.), rabbit anti-TNF-α antibody (Cat. No.: 11948; Cell Signaling Technology, Inc.), rabbit anti-NFATC2 antibody (Cat. No.: 5861; Cell Signaling Technology,

(14) EpCAM activates the ERK-EGR1 signaling axis and promotes TNF-α-induced the progression of anaplastic thyroid cancerJournal of Translational MedicineDecember 22, 2025Chi-Chiu Lee, Yi-Jen Su, Sushree Shankar Panda, Chiung-Yi Chiu, Chung-Hsuan Wu, Tsai-Ming Lu, Pi-Chun Li, Yen-Lin Huang, Ruey-Long Hong, Shao-Hsi Hung, et al.(#2568), OCT4a (#2840), E-cadherin (#3195), Vimentin (#5741), Snail (#3879), Slug (#9585), TNF-α (#11948), and EGR1 (#4154) were obtained from Cell Signaling Technology. Antibodies against human α-tubulin

(15) Flipping the Switch: MeCP2‐Mediated Lactylation Rewires Microglial Metabolism and Inflammation via the HK2/mTOR Axis in Poststroke NeuroinflammationAdvanced ScienceDecember 22, 2025Zengyu Zhang, Shanshan Huang, Yong Wang, Zhiwen Jiang, Zhuohang Liu, Chenran Wang, Rong Ji, Yiwen Yuan, Xueyu Mao, Kaicheng Yang, et al.553 142), Rabbit anti‐iNOS (CST, 13120S), Rabbit anti‐COX‐2 (CST, 12282S), Rabbit anti‐TNF‐α (CST, 11948S), Rabbit anti‐HK2 (Abcam, ab209847), Rabbit anti‐Ki67 (Abcam, ab15580), Rabbit anti‐pH3 (Sigma, 0 6570),

(16) Intermittent exercise alleviates MI-induced renal injury in mice via IGF-1Front PhysiolDecember 19, 2025Wanyu Zhu, Wenyan Bo, Yixuan MaIGF-1 (Abcam, ab133542), IGF-1R (Abcam, ab182408), PI3K (CST, 4257S), AKT (CST, 9272S), TNF-α (CST, 11948S), IL-6 (Abcam, ab9324), Cleaved Caspase-3 (CST, 9661) and GAPDH (Abcam, ab181602). After 2 h at room

(17) Preclinical Evaluation of Atorvastatin-Loaded PEGylated Liposomes in a Mouse Model of Traumatic Brain InjuryInt J Mol SciDecember 18, 2025Eun-Sol Hwang, Ja-Hae Kim, Ji-Hye Kim, Raveena Nagareddy, Yong-Yeon Jeong, Kang-Ho ChoiCell Signaling, 12912s, USA), Iba-1 (1:1000, Cell Signaling, 17198s, USA) and TNF-α (1:1000, Cell Signaling, 11948s, USA) in the BBB on day 5 post-TBI. In immunoblot analyses, β-actin (1:2000, Santa Cruz Biotechnology,

(18) METTL3-mediated m6A modification of TACSTD2 mRNA inhibits papillary thyroid cancer progression by activating the TNF signaling and blocking epithelial–mesenchymal transitionScientific ReportsDecember 18, 2025Jianting Man, Zongyuan Zhang, Tianxing Cui, Tiefeng Shi#46324), MMP2 (CST, #40994), MMP3 (CST, #14351), MMP7 (CST, #3801), MMP9 (CST, #24317), TNF-α (CST, #11948), TNF-R1 (CST, #3736), TNF-R2 (CST, #72337), TRADD (CST, #3684), Bax (CST, #5023), Bcl-2 (CST, #4223),

(19) Inflammation-specific DNA origami nanodevice for delivery of siRNAs to treat ulcerative colitisNature CommunicationsDecember 8, 2025Tianci Zhang, Ruoqing Li, Zhenghao Wang, Ye Zhou, Yaojia Zhou, Xiaoting Chen, Cui Peng, Yi Jiang, Nanwei Tong, Wei Liantigen retrieval and blocking with 5% BSA, and then were incubated overnight at 4 °C with TNF-α (CST, #11948, USA), integrin α4 (CST, #8440, USA), CD3 (Abcam, #ab16669, UK), F4/80 (Abcam, #ab300421, UK) and APE1

(20) Porcine decellularized splenic matrix hydrogel-based vesicle sustained-release System: A dual-regulatory biomaterial for accelerated wound healing via angiogenesis and immune remodelingMaterials today. BioDecember 1, 2025Peng P, Fan S, Liu P, Huang X, Xuan Z, Liu A, Wu M, Sui B, Ye B, Zhang S, et al.Arginase 1(Arg1) antibody (93668s, Cell Signaling Technology, USA; diluted 1:200), Anti-CD4 antibody (11948s, Cell Signaling Technology, USA; diluted 1:200), Anti-CD25 antibody (11948s, Cell Signaling Technology,

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TNF-alpha (D2D4) XP ® Rabbit mAb (Mouse Specific)

TNF-alpha (D2D4) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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TNF-alpha (D2D4) XP ® Rabbit mAb from Cell Signaling Technology

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