PKR (D7F7) Rabbit mAb 12297S from Cell Signaling Technology

Product Specs
ItemPKR (D7F7) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number12297S
Quantity100 µl
ApplicationsW, W-S, IP
ReactivityH
ClonalityMonoclonal
TargetPKR
HostRabbit
NCBI Full Gene Nameeukaryotic translation initiation factor 2 alpha kinase 2
NCBI Gene AliasesDYT33, EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR
Research AreaTranslational Control
IsotypeIgG
CloneD7F7
Molecular Weight74

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) CAV2 Modulates Cetuximab Sensitivity in HNSCC via Ubiquitin-Mediated Disruption of the PACT-PKR AxisCancers (Basel)April 2, 2026Yun Wang, Yafei Wang, Dongqi Yuan, Shenge Liu, Peng Chenantibodies were employed: CAV2 (1:1000, NBP1-31116, Novus Biologicals, Centennial, CO, USA), PKR (1:1000, 12297S, CST, Danvers, MA, USA), PACT (1:1000, 13490S, CST, Danvers, MA, USA), Ki67 (62548SF, 1:1000, CST, Danvers,

(2) SARS-CoV-2 Nsp15 facilitates immune evasion and viral replication by limiting multiple host innate immune pathways, including cGAS-STINGJ Gen VirolMarch 25, 2026Hsin-Ping Chiu, Yao Yu Yeo, Tsoi Ying Lai, Chuan-Tien Hung, Shreyas Kowdle, Griffin D. Haas, Sizun Jiang, Weina Sun, Benhur Leeanti-GAPDH (ab8245) from Abcam; rabbit anti-STAT1 (#14994), rabbit anti-peIF2α (#3398), rabbit anti-PKR (#12297), rabbit anti-V5 (#13202), mouse anti-FLAG (#8146) and rabbit anti-COXIV (#4850) from Cell Signaling Technology

(3) USP30-AS1 functions as a post-transcriptional amplifier of interferon signalling to drive autoimmune pathogenesisCell & BioscienceFebruary 26, 2026Jie Pan, Min-Yi Feng, Tian Xia, Shuai Jiang, Peng-Peng Zhu, Xi-Ping Yu, Zhi-Hui Su, Yu Liu, Jia-Qi Wu, Ming Zhao, et al.(sc-515235) and anti-β-Actin (sc-47778) were from Santa Cruz Biotechnology; anti-MDA5 (5321S) and anti-PKR (12297S) were from Cell Signalling Technology; anti-GAPDH (10494-1-AP) was from Proteintech Group; anti-RIG-I

(4) Overcoming the eIF2α Brake in Human Cell-Derived Translation SystemsbioRxivJanuary 16, 2026Nikolay A. Aleksashin, Rohan R. Shelke, Tianhao Yin, Jamie H. D. Cateband. Antibodies The following antibodies were used in this study. Antibodies for eIF2𝘢 (9722S), PKR D7F7 (12297T), phospho-eIF2𝘢 (S51) (9721S), eEF2 (2332S) and phospho-eEF2 (T56) (2331S) were from Cell Signaling Technology

(5) Directed evolution generates an oncolytic Sindbis virus with enhanced tumor-killing capacity in osteosarcomaMolecular Therapy OncologyDecember 18, 2025Kangyixin Kangyixin, Qinghan Qinghan, Li Li, Hongjun Hongjun, Zijun Zijun, Yang Yang, Jia Fan, Fuqiang Fuqiangantiphospho-STAT1 (Cell Signaling Technology, no. 9167), rabbit monoclonal anti-PKR (Cell Signaling Technology, no. 12297), rabbit monoclonal antiphospho-PKR (Abcam, no. ab32036), HRP-conjugated goat anti-rabbit IgG (Proteintech,

(6) SARS-CoV-2 Nsp15 endoribonuclease subverts host defenses to enhance viral fitness in lung cellsJournal of VirologySeptember 23, 2025Xiang Chi, Xueying Liang, Kishore Vaddadi, Xiaoming Zhang, Chaitanya Gandikota, Sunil More, Lin Liu, Xufang Dengsignal, 9722S","1:1,000" Phospho-PKR (Thr446),"Fisher, PIMA532086","1:1,000" PKR,"Cell signaling, 12297S","1:1,000" Goat anti-rabbit IgG,"Fisher, OB4030-05","1:7,000" Goat anti-mouse IgG,"Fisher, OB1010-05","1:7,000" Sources

(7) The SHFV nsp2 and nucleocapsid proteins recruit G3BP1 to sites of viral replication, but stress granules are not induced by the infectionJournal of VirologyJuly 22, 2025Ayisha A. Lavender, Oreoluwa Solanke, Hsin-Yao Tang, Hannah E. Peck, Daryll Vanover, Philip J. Santangelo, Margo A. Brintonat 4°C while rocking. The antibodies used were rabbit anti-PKR (1:400, Cell Signaling Technology, #12297), rabbit anti-p-PKR (1:500, Invitrogen, #MA5-38282), mouse anti-eIF2α (1:500, Abcam, #5369), rabbit

(8) Effective recognition of double-stranded RNA does not require activation of cellular inflammationSci AdvApril 11, 2025Karolina Drazkowska, Julia Cieslicka, Michal Kitowicz, Anna Pastucha, Lukasz Markiewicz, Wiktoria Szymanek, Krzysztof Goryca, Tomasz Kowalczyk, Dominik Cysewski, Andreas R. Bausch, et al.RRID:AB_2724898), rabbit anti-OAS3 (Invitrogen, PA5-59494, RRID:AB_2644932), rabbit anti-PKR (Cell Signaling, 12297S, RRID:AB_2665515), mouse anti-Puromycin (Sigma-Aldrich, MABE343, RRID:AB_2566826), rabbit anti-RIG-I

(9) PACT prevents aberrant activation of PKR by endogenous dsRNA without sequestrationNat CommunApril 8, 2025Sadeem Ahmad, Tao Zou, Jihee Hwang, Linlin Zhao, Xi Wang, Anton Davydenko, Ilana Buchumenski, Patrick Zhuang, Alyssa R. Fishbein, Diego Capcha-Rodriguez, et al.to pre-equilibrated protein G Dyanbeads (Invitrogen) along with Anti-HA (CST #3724), Anti-PKR (CST #12297), or Anti-Flag (Millipore Sigma #F1804) antibody. The mixture was incubated at 4°C for 2 h before washing

(10) Coronavirus endoribonuclease nsp15 suppresses host protein synthesis and evades PKR-eIF2α-mediated translation shutoff to ensure viral protein synthesisPLOS PathogensMarch 17, 2025Xiaoqian Gong, Shanhuan Feng, Jiehuang Wang, Bo Gao, Wenxiang Xue, Hongyan Chu, Shouguo Fang, Yanmei Yuan, Yuqiang Cheng, Min Liao, et al.anti-Flag (Gentaur, #AFLAG), rabbit anti-phosphorylated PKR (Abcam, #ab32036), rabbit anti-PKR (CST, #12297), rabbit anti-phosphorylated eIF2α (CST, #3398), rabbit anti-eIF2α (CST, #5324), rabbit anti-RPS6 mAb

(11) RNA sensing induced by chromosome missegregation augments anti-tumor immunityMolecular CellFebruary 20, 2025Nobunari Sasaki, Mizuki Homme, Takahiko Murayama, Tatsuya Osaki, Toshiyuki Tenma, Tadaichi An, Yujiro Takegami, Tetsuo Tani, Patrick C. Gedeon, Yoshihisa Kobayashi, et al.anti-phospho-PKR,abcam,Cat# ab32036 RRID: AB_777310 Rabbit monoclonal anti-PKR,Cell signaling technology,Cat# 12297S RRID: AB_2665515 Rabbit polyclonal anti-MAVS,Cell signaling technology,Cat# 3993S RRID: AB_823565 Rabbit

(12) DHX15 and Rig-I Coordinate Apoptosis and Innate Immune Signaling by Antiviral RNase LVirusesDecember 13, 2024Barkha Ramnani, Trupti Devale, Praveen Manivannan, Aiswarya Haridas, Krishnamurthy Malathicaspase-3 (9664), FLAG (1479), Myc (2272), Phospho-JNK (9251), Total-JNK (9252), Phospho-p38 (9211), PKR (12297), RIG-I (3743), P-cJun (9261), P-MAPKAPK-2 (3007), IRF-3 (4302), Lamin A/C (2032), and β-actin (3700)

(13) Circ-CAMTA1 regulated by Ca(2+) influx inhibited pyruvate carboxylase activity and modulate T cell function in patients with systemic lupus erythematosusArthritis research & therapyOctober 29, 2024Yu HC, Tseng HH, Huang HB, Lu MC.NFAT1 (#4389, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal antibodies against PKR (#12297, Cell Signaling Technology, Danvers, MA, USA), and rabbit polyclonal antibodies against pyruvate carboxylase

(14) SARS-CoV-2 Nsp15 antagonizes the cGAS-STING-mediated antiviral innate immune responsesbioRxiv : the preprint server for biologySeptember 5, 2024Chiu HP, Yeo YY, Lai TY, Hung CT, Kowdle S, Haas GD, Jiang S, Sun W, Lee B.anti-GAPDH (ab8245) from Abcam. Rabbit anti-STAT1 (#14994), rabbit anti-peIF2α (#3398), rabbit anti-PKR (#12297), rabbit anti-V5 (#13202), mouse anti-FLAG (#8146), and rabbit anti-COXIV (#4850) from Cell Signaling Technology

(15) RNA helicase SKIV2L limits antiviral defense and autoinflammation elicited by the OAS-RNase L pathwayThe EMBO JournalAugust 7, 2024Kun Yang, Beihua Dong, Abhishek Asthana, Robert H Silverman, Nan Yan,Cell Signaling,5625 PKR (phospho T446) antibody [E120],Abcam,ab32036 PKR (D7F7) Rabbit mAb,Cell Signaling,12297 Phospho-eIF2α (Ser51) (119A11) Rabbit mAb,Cell Signaling,3597 eIF2α (D7D3) XP® Rabbit mAb,Cell Signaling,5324 ADAR1

(16) The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphateThe EMBO journalJuly 1, 2024Bhattacharjee P, Wang D, Anderson D, Buckler JN, de Geus E, Yan F, Polekhina G, Schittenhelm R, Creek DJ, Harris LD, et al.(Millipore). Membranes were probed with primary antibodies; anti-PKR (sc-6282, Santa Cruz; EPR19374, Abcam; #12297 D7F7, Cell Signaling Technology or BC1 (Zamanian-Daryoush et al, 1999)), -G6PD (#12263 D5D2, Cell Signaling Technology

(17) Genetic alteration of SJ293TS cells and modification of serum-free media enhances lentiviral vector productionMolecular Therapy - Methods & Clinical DevelopmentJune 13, 2024Matthew Bauler, Francesca Ferrara, Brandon Lowe, Jordan A. Beard, Chris Wincek, Matthew M. Wielgosz, Jeoungeun J. Park, Na Shang, Saikat Nandy, Cai Li, et al.gel electrophoresis and blotting. The primary antibodies used were rabbit anti-PKR (Cell Signaling, Danvers, MA, #12297; clone D7F7), rabbit anti-B2M (Cell Signaling, #12851; clone D8P1H), mouse anti-Gapdh (Santa Cruz Biotechnology,

(18) Comparison of SARS-CoV-2 variants of concern in primary human nasal cultures demonstrates Delta as most cytopathic and Omicron as fastest replicatingmBioApril 10, 2024Tanneti NS, Patel AK, Tan LH, Marques AD, Perera RAPM, Sherrill-Mix S, Kelly BJ, Renner DM, Collman RG, Rodino K, et al.(anti-rabbit, 1:1000, Abcam Cat. No. 32036), PKR (anti-rabbit, 1:1000, Cell Signaling Technology Cat. No. 12297S), p-eIF2α (anti-rabbit, 1:1000, Cell Signalining Technology Cat. No. 9721S), eIF2α (anti-rabbit, 1:1000,

(19) Chronic endoplasmic reticulum stress in myotonic dystrophy type 2 promotes autoimmunity via mitochondrial DNA releaseNature communicationsFebruary 20, 2024Rösing S, Ullrich F, Meisterfeld S, Schmidt F, Mlitzko L, Croon M, Nattrass RG, Eberl N, Mahlberg J, Schlee M, et al.sc-47778, 1:2000 and Licor 926-42212, 1:1000), α-Tubulin (Neomarker MS-581-P1, 1:1000) PKR (Cell Signaling #12297, 1:1000), phospho-STAT1 (Cell Signaling #9167, 1:1000) and cleaved caspase 3 (Cell Signaling #9661,

(20) G3BP1-dependent condensation of translationally inactive viral RNAs antagonizes infectionScience advancesFebruary 2, 2024Burke JM, Ratnayake OC, Watkins JM, Perera R, Parker R.(Cell Signaling Technology, 2118 L) was used at 1:2000 for IB analysis. Rabbit anti-PKR (Cell Signaling Technology, 12297S) was used at 1:1000 for IB analysis. Rabbit polyclonal anti-PABP antibody (Abcam, ab21060) was used

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PKR (D7F7) Rabbit mAb

PKR (D7F7) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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PKR (D7F7) Rabbit mAb from Cell Signaling Technology

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