Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb 8599S from Cell Signaling Technology

Product Specs
ItemPhospho-Akt2 (Ser474) (D3H2) Rabbit mAb
CompanyCell Signaling Technology
Price $401.00 Inquire
Catalog Number8599S
ApplicationsWB, IP
ReactivityHu, Ms, Rt
ModificationsAkt2 (Ser474) phosphate
Research AreaPI3K / Akt Signaling
IsotypeIgG
CloneD3H2
Molecular Weight60
NCBI Gene AliasesHIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA
NCBI Full Gene NameAKT serine/threonine kinase 2
HostRabbit
TargetAkt2 (Ser474) phosphate
ClonalityMonoclonal
Quantity100 µl

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Cytoskeletal Protein 4.1R Inhibits BCR-Mediated B-Cell Activation by Restraining AKT1 PhosphorylationCellsJuly 13, 2026Yuying Guo, Dandan Fan, Denghui Liu, Qi Shao, Siyao Sang, Yuting Niu, Lixiang Chen, Taotao Liang1:1000), anti-AKT2 (2964, Cell Signaling Technology, Danvers, MA, USA; 1:1000), anti-Phospho-AKT2 (8599, Cell Signaling Technology, Danvers, MA, USA; 1:1000), anti-pan-AKT (9272, Cell Signaling Technology,

(2) Protein Tyrosine Phosphatase 1B-Mediated Granulosa Cell Insulin Resistance Links Metabolic Stress to Aging-Relevant Ovarian Dysfunction and Is Reversed by GengnianchunAging CellJune 9, 2026Yanqiu Rao, Ting Xu, Yan Ding, Jun Li, Lingyun Gao, Yun Wang, Wenjun WangPTP1B (Proteintech, Cat# 11334‐1‐AP, 1:100), phospho‐AKT2 (Ser474) (Cell Signaling Technology, Cat# 8599, 1:200), and GLUT4 (Proteintech, Cat# 66846‐1‐Ig, 1:200), followed by appropriate HRP‐conjugated secondary

(3) Optineurin modulates mTORC2/AKT/STAT3 signaling to control MHC class II expression and adaptive immunity against HSV-1Cell ReportsApril 16, 2026Rashmi Kadam, Chandrashekhar Patil, Leonid Feferman, Mark Maienschein-Cline, George Chlipala, Piyush Borole, Ilina Bhattacharya, Chima Orameh, Tara Nyugen, Henry Tseng, et al.,Cell Signaling Technology,Cat#9018 RRID:AB_2629283 Rabbit monoclonal anti-pAKT2 (S474),Cell Signaling Technology,Cat#8599 RRID:AB_2630347 Rabbit monoclonal anti-pRICTOR,Cell Signaling Technology,Cat#3806 RRID:AB_10557237 Rabbit

(4) Nr4a1 regulates microglia overactivation-mediated neuroinflammation to ameliorate cognitive impairment in trigeminal neuralgia ratsThe Journal of Headache and PainFebruary 20, 2026Junjie Li, Zhilun Yang, Xiaojuan Mi, Qiang Wang, Juan Ding, Ziqi Feng, Xia Ma, Liping Zhang, Juan Liuincubated overnight at 4 °C with primary antibodies against Iba1 (1:300; ab283319, Abcam), p-Akt2 (1:200; 8599, Cell Signaling Technology), or Postsynaptic density protein 95 (PSD95) (1:200; 3409, Cell Signaling Technology

(5) Vav1 Sustains the Expression of Insulin, PDX1 and miR-375 During Differentiation of hiPSCs to β Cells: A Potential Target to Improve the In Vitro Generation of Insulin-Producing CellsTissue Engineering and Regenerative MedicineFebruary 1, 2026Marina Pierantoni, Valentina Zamarian, Federica Brugnoli, Silvia Grassilli, Laura Monaco, Marcello Dell’Aira, Valeria Sordi, Valeria Bertagnolo(16364-1-AP Proteintech, Manchester, UK), PDX1 (AF2419 R&D Systems, Minneapolis, MN, USA), pSer474-Akt2 (8599S) and Akt2 (3063 s) from Cell Signaling Technology, pSer473-Akt1 (05-736 Merck Millipore, Milan, Italy),

(6) Non-redundant roles of the phosphoinositide phosphatases PTEN and PIPP in PI3K/AKT signaling in breast cancerCommunications BiologyDecember 17, 2025Lisa M. Ooms, Daniel T. Ferguson, Samuel J. Rodgers, Karmanpreet K. Sukhija, Emily I. Jones, Mariah P. Csolle, Hon Yan Kelvin Yip, Roger J. Daly, Tony Tiganis, Catriona A. McLean, et al.were from: pAKTThr308 (#2965), pAKTSer473 (#4058), AKT (#4685), pAKT1Ser473 (#9018), pAKT2Ser474 (#8599), pPRAS40Thr246 (#2997), PRAS40 (#2691), pS6Ser235/236 (#4858), pS6Ser240/244 (#5364), HA (#3724), PI3K

(7) SerpinA3k Deficiency Ameliorates Experimental type 2 DiabetesCell Mol Life SciDecember 2, 2025Isaac González-Soria, Esmeralda Palacios-Brito, Dulce Gómez-Trujillo, Karla Barragán-Jiménez, Axel D. Soto-Valadez, Miguel Angel Martínez-Rojas, Daniel Oliva-García, Jorge Aranda, Rosalba Pérez-Villalva, Lorena López-Griego, et al.against SerpinA3k (1:1,000; Cat. 55480-1-AP, Proteintech, IL. USA), pAKT2 Ser474 (1,2,000; Cat. No, 8599 S, Cell Signaling, MA, USA), totalAKT2 (1:2,000; Cat. No. 3063 S, Cell Signaling, MA, USA) and interleukin

(8) Androgen deprivation–mediated activation of AKT is enhanced in prostate cancer with TMPRSS2:ERG fusionJournal of Clinical InvestigationDecember 1, 2025Fen Ma, Sen Chen, Luigi Cecchi, Betul Ersoy-Fazlioglu, Joshua W. Russo, Seiji Arai, Seifeldin Awad, Carla Calagua, Fang Xie, Larysa Poluben, et al.9018, 1:1,000), AKT2 (Cell Signaling Technology, 3063, 1:1,000), AKT2 pS474 (Cell Signaling Technology, 8599, 1:1,000), PTEN (Cell Signaling Technology, 9559, 1:1,000), PHLPP1 (Abcam, ab305295, 1:1,000), and PHLPP2

(9) Dual Role of Diallyl Disulfide (DADS) on Invasive Potential and β-Catenin Dynamics in HER2-Positive Breast Cancer CellsCancers (Basel)November 5, 2025Marcello Dell’Aira, Silvia Grassilli, Marina Pierantoni, Valeria Bertagnolo, Federica BrugnoliSanta Cruz Biotechnology (Heidelberg, Germany); Akt (AF2055), p-Akt (#3787S), Akt2 (#3063), p-Akt2 (Ser474, #8599), p-GSK-3β (Ser9, #5558) and GSK-3β (#9315) from Cell Signaling Technology (Danvers, MA, USA); against

(10) Androgen deprivation–mediated activation of AKT is enhanced in prostate cancer with TMPRSS2:ERG fusionJ Clin InvestOctober 2, 2025Fen Ma, Sen Chen, Luigi Cecchi, Betul Ersoy-Fazlioglu, Joshua W. Russo, Seiji Arai, Seifeldin Awad, Carla Calagua, Fang Xie, Larysa Poluben, et al.9018, 1:1,000), AKT2 (Cell Signaling Technology, 3063, 1:1,000), AKT2 pS474 (Cell Signaling Technology, 8599, 1:1,000), PTEN (Cell Signaling Technology, 9559, 1:1,000), PHLPP1 (Abcam, ab305295, 1:1,000), and PHLPP2

(11) AKT2 Modulates Astrocytic Nicotine Responses In VivoGliaOctober 1, 2025Andrew M. Lombardi, Mina Griffioen, Helen Wong, Ryan Milstead, Curtis Borski, Erin Shiely, Myra E. Bower, Emily Schmitt, Lauren LaPlante, Marissa A. Ehringer, et al.non-specific band was excluded from analysis. The phospho-AKT2 Ser474 (pAKT2) antibody (Cell Signaling, 8599; diluted 1:750) recognized only the expected band at 60 kDa on western blots of mouse brains, consistent

(12) Tissue-Specific Enhancement of Insulin Function and Restoration of Glucose-Stimulated Insulin Secretion by Croton guatemalensis Lotsy and Eryngium cymosum F. DelarochePharmaceuticals (Basel)September 24, 2025Fernanda Artemisa Espinoza-Hernández, Angelina Daniela Moreno-Vargas, Andrea Díaz-Villaseñor, Gerardo Mata-Torres, Jazmín Samario-Román, Adolfo Andrade-Cettoprimary antibodies anti-Akt cat. 9272, anti-pAkt1 (Ser473) cat. 9018, and anti-pAkt2 (Ser474) cat. 8599 (Cell Signaling Technology, Danvers, MA, USA). Dilutions for liver protein: anti-Akt 1:300, anti-pAkt1

(13) Akt isoform specificity drives intrinsic immune regulation during HSV-1 infectionProc Natl Acad Sci U S AJuly 8, 2025Rahul K. Suryawanshi, Chandrashekhar D. Patil, Hemant Borase, Deepak Shukla(2938) 1:500, Phospho-Akt1 (Ser473) (9018) 1:500, Akt2 (D6G4) (3063) 1:1,000, and Phospho-Akt2 (Ser474) (8599) 1:500, all purchased from Cell Signaling Technology (Danvers, MA); GAPDH (10494-1-AP) 1:2,000 was purchased

(14) Microneurotrophin BNN27 Exerts Significant Anti-Inflammatory Effects on Murine T-Lymphocytes Following CFA-Induced Inflammatory PainInt J Mol SciJune 8, 2025Smaragda Poulaki, Aikaterini Kalantidou, Ioanna Lapi, Achille Gravanis, Maria Venihakiantibodies for AKT1 and AKT2 (total/phospho 1:1000, rabbit; Cell Signaling, Danvers, MA, cat# 2964S and 8599S, respectively). To detect the bands of interest, we used the Benchmark Pre-Stained Protein Standard

(15) Dietary oleic acid drives obesogenic adipogenesis via modulation of LXRα signalingCell ReportsApril 22, 2025Allison Wing, Elise Jeffery, Christopher D. Church, Jennifer Goodell, Rocío del M. Saavedra-Peña, Moumita Saha, Brandon Holtrup, Maud Voisin, N. Sima Alavi, Mariana Floody, et al.Akt2, 1:1000",Cell Signaling,"3063, RRID:AB_2225186" "Rabbit phospho-Akt2, 1:500",Cell Signaling,"8599, RRID:AB_2630347" "Anti-rabbit HRP, 1:10,000",Jackson ImmunoResearch,"211-032-171, RRID:AB_2339149" "Phospho-AKT

(16) S100A8/A9 innate immune signaling as a distinct mechanism driving progression of smoking-related breast cancersOncogeneJanuary 25, 2025Samson Mugisha, Shahnawaz A. Baba, Shreyas Labhsetwar, Devam Dave, Aran Zakeri, Richard Klemke, Jay S. Desgrosellierantibodies were used for immunoblotting at the following dilutions: 1:1000 S100A9 (Cat# 72590), p-AKT (Cat# 8599), total Akt (Cat# 3063), p-STAT3 (Cat# 9131), total STAT3 (Cat# 9139), p-ERK(1/2) (Cat# 4370), total

(17) Pleiotropic tumor suppressive functions of PTEN missense mutations during gliomagenesisiScienceDecember 20, 2024Hyun Jung Jun, Joao A. Paulo, Victoria A. Appleman, Tomer M. Yaron-Barir, Jared L. Johnson, Alan T. Yeo, Vaughn A. Rogers, Shan Kuang, Hemant Varma, Steven P. Gygi, et al.,Cell Signaling Technology,9018 anti-AKT1,Cell Signaling Technology,2938 anti-AKT2Ser-474,Cell Signaling Technology,8599 anti-AKT2,Cell Signaling Technology,3063 anti-PRAS40-Thr-246,Cell Signaling Technology,2997 anti-PRAS40,Cell Signaling Technology

(18) Cancer cell stiffening via CoQ(10) and UBIAD1 regulates ECM signaling and ferroptosis in breast cancerNature communicationsSeptember 18, 2024Tosi G, Paoli A, Zuccolotto G, Turco E, Simonato M, Tosoni D, Tucci F, Lugato P, Giomo M, Elvassore N, et al.Cell Signaling Cat. #9018, RRID:AB_2629283), anti-human p-AKT2 (Ser474) (1:1000, Cell Signaling Cat. #8599, RRID:AB_2630347), anti-human GSK-3β (1:1000, Cell Signaling Cat. #9832, RRID:AB_10839406), anti-human

(19) CRLF1 bridges AKT and mTORC2 through SIN1 to inhibit pyroptosis and enhance chemo-resistance in ovarian cancerCell Death & DiseaseSeptember 10, 2024Cong Xiang, Li Chen, Shilei Zhu, Yue Chen, Haodong Huang, Chunmao Yang, Yugang Chi, Yanzhou Wang, Yunlong Lei, Xiongwei Caispecific for Akt (9272), phospho-Akt (Ser473, 4060S), phospho-Akt (Thr308, 13038S), phospho-Akt2 (Ser474, 8599S), phospho-ASK1 (Thr845, 3765S), phospho-FOXO1, phospho-S6K, S6K, mTOR (2983S), Cleaved Caspase-3 (9661S),

(20) Carvedilol Activates a Myofilament Signaling Circuitry to Restore Cardiac Contractility in Heart FailureJACC: Basic to Translational ScienceAugust 1, 2024Ying Wang, Meimi Zhao, Xianhui Liu, Bing Xu, Gopireddy R. Reddy, Aleksandra Jovanovic, Qingtong Wang, Chaoqun Zhu, Heli Xu, Elizabeth F. Bayne, et al.Cell Signaling Technology), anti-MYPT1 (1:500; 8574, Cell Signaling Technology), anti-phospho-Akt2 serine 474 (1:1,000; 8599, Cell Signaling Technology), anti-phospho-Akt1 serine 473 (1:1,000; 9018, Cell Signaling Technology),

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Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific)

Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb from Cell Signaling Technology

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