Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab Rabbit mAb mix 9477S from Cell Signaling Technology

Product Specs
ItemPhospho-CDK Substrate Motif [(K/H)pSP] MultiMab Rabbit mAb mix
CompanyCell Signaling Technology
Price $431.00 Inquire
Catalog Number9477S
Quantity100 µl
ApplicationsW, IP, E-P
ReactivityAll
ClonalityMonoclonal
HostRabbit
ModificationsCDKs Substrate (Ser) phosphate
Research AreaMotif Antibodies
IsotypeIgG

Questions and Answers

Can this antibody be used to study the phosphorylation of proteins in the context of viral infections?

Yes, the Phospho-CDK Substrate Motif MultiMab has been utilized in studies examining the phosphorylation of viral proteins, such as the p12 protein of murine leukemia virus, which is critical for its life cycle and nuclear entry. The antibody has been shown to detect specific phosphorylated motifs that are essential for viral function ().

Is this antibody effective for use in immunoprecipitation experiments?

The Phospho-CDK Substrate Motif MultiMab has been successfully employed in immunoprecipitation assays, as indicated in various studies. For instance, it was used to analyze the phosphorylation status of proteins involved in cell cycle regulation and DNA repair pathways, demonstrating its utility in such applications (, ).

What types of samples can this antibody be applied to?

This antibody is versatile and has been shown to work across a wide range of species, including human, mouse, zebrafish, and various other vertebrates and invertebrates. Its broad reactivity makes it suitable for diverse biological studies ().

How has this antibody been utilized in studies related to cell cycle regulation?

The Phospho-CDK Substrate Motif MultiMab has been referenced in studies investigating the role of cyclin-dependent kinases in regulating cell cycle progression. For example, it has been used to assess the phosphorylation of proteins involved in DNA repair and cell cycle checkpoints, highlighting its relevance in understanding cell cycle dynamics (, ).

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Website: http://www.cellsignal.com/

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(1) UVB-induced genotoxic stress activates the DNA damage response and innate immune pathways in sea urchin coelomocytesFrontiers in ImmunologyMarch 19, 2026Riss M. Kell, Margaret K. Weber, Rosa Y. Escalante, Jeffrey C. Silva, Andrea G. Bodnar(Cell Signaling Technology, mix of #9614 and #10001), phospho-MAPK/CDK Substrate Motif (Cell Signaling Technology, mix of #9477 and #2325), and phospho-PKC Substrate Motif (Cell Signaling Technology, #6967). β-actin rabbit polyclonal

(2) Geminin inhibits DNA replication licensing by sterically blocking CDT1-MCM2 interactionsNat CommunDecember 9, 2025Joshua Tomkins, Lucy V. Edwardes, Sarah V. Faull, Matthew Peach, Peter J. Gillespie, Vera Leber, Anna Schmidt, Halil Bounoua, Nicholas Sim, Rosa Camarillo, et al.above, but with Phospho-CDK Substrate Motif [(K/H)pSP] primary antibody (Cell Signalling Technology, #9477 lot 2, 1:1000) and anti-rabbit IgG HRP-linked secondary antibody (Cell Signalling Technology, #7074,

(3) Unveiling an Arpp-19 phosphorylation switch that grants chromosome stabilityCommun BiolMay 30, 2025Angela Flavia Serpico, Caterina Pisauro, Asia Trano, Domenico Grieco(CREST; Cat# 15-234, Antibodies Incorporated; 1:100), rabbit anti-Cdk substrate motif [(K/H)pSP] (Cat# 9477, Cell Signaling Technology; 1:1000), anti-Flag Peroxidase (HRP) (Cat# A8592, Sigma-Aldrich; 1:500),

(4) CDK4/6-mediated phosphorylation of DUB3 promotes YAP1 stability and hepatocellular carcinoma progressionCell Death DiscovApril 30, 2025Lei Huang, Wenying Yuan, Xinying Li, Yixia Liu, Rui Wan, Xiuqing Ma, Tongzheng Liu, Junjie Liang, Yingjie ZhuAnti-ubiquitin (sc-8017, dilution: 1:300) antibody was purchased from Sigma-Aldrich. Anti-CDK substrate (9477S, dilution: 1:500), Anti-CDK4 (12790, dilution: 1:1,000) and Anti-CDK6 (13331, dilution: 1:1,000) antibodies

(5) Lineage-specific CDK activity dynamics characterize early mammalian developmentCell ReportsApril 22, 2025Bechara Saykali, Andy D. Tran, James A. Cornwell, Matthew A. Caldwell, Paniz Rezvan Sangsari, Nicole Y. Morgan, Michael J. Kruhlak, Steven D. Cappell, Sergio RuizSox2,Cell Signaling,"23064S, RRID:AB_2714146" Rabbit Anti Phospho-CDK substrate Motif,Cell Signaling,"9477, RRID:AB_2714143" Goat Anti Gata6,R&D systems,"AF1700, RRID:AB_2108901" Rabbit Anti Nanog,Abcam,"ab80892,

(6) Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regenerationNat CommunMarch 24, 2025Dongcheng Cai, Chungeng Liu, Haotong Li, Chiyin Wang, Lina Bai, Jie Feng, Miaoqing Hu, Hao Wang, Shen Song, Yifan Xie, et al.82669-2-RR, USA), and anti-Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab (1:1000, Cell Signaling Technology 9477, USA). anti-Flag (1:1000, Sigma Aldrich F7425), and anti-β-tubulin (1:1000, Cell Signaling Technology

(7) High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cellsNat CommunMarch 16, 2025Camilla Rega, Ifigenia Tsitsa, Theodoros I. Roumeliotis, Izabella Krystkowiak, Maria Portillo, Lu Yu, Julia Vorhauser, Jonathon Pines, Jörg Mansfeld, Jyoti Choudhary, et al.Scientific, A301-175A, 1:1000), Phospho-CDK substrate motif [(K/H)pSP] (Cell Signalling Technology, 9477, 1:1000), JUNB (Proteintech, 10486-1-AP, 1:1000), CHAF1B (Thermo Fisher Scientific, A301-085A, 1:2000),

(8) Targeting CDK4/6 suppresses colorectal cancer by destabilizing YAP1MedComm (2020)February 17, 2025Yalei Wen, Xiao Yang, Shengrong Li, Lei Huang, Jiayi Chen, Lirong Tan, Xiuqing Ma, Yingjie Zhu, Zhengqiu Li, Changliang Shan, et al.1:1000), CTGF (625474‐1‐AP, dilution: 1:1000) were from Proteintech Group. Phospho‐CDK substrate antibody (9477, 1:500), K48 or K63‐linkage Specific Polyubiquitin Rabbit mAb (8081, 5621, 1:1000) were from Cell Signaling Technology

(9) Revisiting phosphoregulation of Cdc25C during M-phase inductioniScienceJanuary 17, 2025Tan Tan, Chuanfen Wu, Ruoning Wang, Bih-Fang Pan, David Hawke, Fumin Yin, Zehao Su, Boye Liu, Sue-Hwa Lin, Wei Zhang, et al.Antibodies used to immunoblot K/HpSp and RXXpS/T motifs were obtained from Cell Signaling Technology (9477 and 9614). Horseradish peroxidase-conjugated goat anti-rabbit or goat anti-mouse IgG antibodies from

(10) CDK5–cyclin B1 regulates mitotic fidelityNatureSeptember 26, 2024Xiao-Feng Zheng, Aniruddha Sarkar, Humphrey Lotana, Aleem Syed, Huy Nguyen, Richard G. Ivey, Jacob J. Kennedy, Jeffrey R. Whiteaker, Bartłomiej Tomasik, Kaimeng Huang, et al.IB; phospho-Ser/Pro antibody: rabbit phospho (Ser)CDKs substrate P-S2–100 Cell Signaling Technology 9477S 10μL was used per IP; rabbit PP4R3/SMEK2 Abcam Ab224222, 1:50 for IF; PP4R3β/SMEK2 rabbit phospho-S840

(11) CDK phosphorylation of Sfr1 downregulates Rad51 function in late-meiotic homolog invasionsThe EMBO JournalAugust 22, 2024Inés Palacios-Blanco, Lucía Gómez, María Bort, Nina Mayerová, Silvia Bágeľová Poláková, Cristina Martín-Castellanos(1:3000, Living Colors, Clontech), rabbit monoclonal mix anti-P(S)CDK Substrate antibodies (1:1000, 9477 Cell Signaling), monoclonal anti-Cdc2 Y100.4 (1:3000, Santa Cruz), anti-mouse HRP-conjugated antibodies

(12) Hippo-PKCζ-NFκB signaling axis: A druggable modulator of chondrocyte responses to mechanical stressiScienceJune 21, 2024Xiaomin Cai, Christopher Warburton, Olivia F. Perez, Ying Wang, Lucy Ho, Christina Finelli, Quinn T. Ehlen, Chenzhou Wu, Carlos D. Rodriguez, Lee Kaplan, et al.,Cell Signaling Technology,Cat# 5759; RRID: AB_10949320 p-CDK substrates,Cell Signaling Technology,Cat# 9477; RRID: AB_2714143 p-ATM/ATR substrates,Cell Signaling Technology,Cat# 6966; RRID: AB_10949894 pT202/Y204ERK1/2,Cell Signaling Technology

(13) Maintaining Drosha expression with Cdk5 inhibitors as a potential therapeutic strategy for early intervention after TBIExperimental & Molecular MedicineJanuary 10, 2024Lu Huang, Li Xia, Tiejian Nie, Bozhou Cui, Jianjun Lu, Fangfang Lu, Feiyan Fan, Dongni Ren, Yuan Lu, Guodong Gao, et al.CST), cleaved Caspase 3 (1:1000, 9661 S, CST), p-Tau (1:800, 9632 S, CST), α-p/s-substrate (1:1000, 9477 S, CST), β-actin (1:5000, AC026, ABclonal) and FLAG (1:1000, 70586SF, CST). Histopathological analysis For

(14) Synergistic anticancer effect by targeting CDK2 and EGFR–ERK signalingJournal of Cell BiologyJanuary 1, 2024Jinhuan Wu, Yuping Chen, Rui Li, Yaping Guan, Mu Chen, Hui Yin, Xiaoning Yang, Mingpeng Jin, Bingsong Huang, Xin Ding, et al.Proteintech), anti-phosphor-Histone-H3 (ab5176; Abcam), anti-cyclinA2 (67955S; CST), anti-P-CDK substrate (9477S; CST), anti-RSK (9333S; CST), anti-MSK (3489S; CST), anti-Phospho-p90RSK (Ser380; 9341S; CST), anti-Phospho-MSK1 (Thr581

(15) Synthetic lethal interaction between WEE1 and PKMYT1 is a target for multiple low-dose treatment of high-grade serous ovarian carcinomaNAR CancerJune 13, 2023Jan Benada, Daria Bulanova, Violette Azzoni, Valdemaras Petrosius, Saba Ghazanfar, Krister Wennerberg, Claus Storgaard SørensenCell Signaling Technology, cat#9111S; phospho-CDK substrate motif 1:1000, Cell Signaling Technology, cat#9477; CHK2 1:500, Santa Cruz, cat#sc-56296; CHK2pT18 1:500, Cell Signaling Technology, cat#2661; CHK1 1:500,

(16) CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growthCell reportsApril 25, 2023Yin S, Liu L, Ball LE, Wang Y, Bedford MT, Duncan SA, Wang H, Gan W.14145; RRID:AB_2773717 Rabbit monoclonal phosphor-CDK substrate motif,Cell Signaling Technology,Cat# 9477; RRID:AB_2714143 Phospho-MAPK/CDK Substrates,Cell Signaling Technology,Cat# 2325 Mouse monoclonal

(17) Protocol to assess substrate dephosphorylation by serine/threonine phosphoprotein phosphatases in vitroSTAR protocolsApril 18, 2023Wasserman JS, Feiser F, Palacio S, Patel K, Gonzalez J, Fowle H, Graña X.,Cell Signaling Technology,2290S Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab (rabbit monoclonal)1:1000,Cell Signaling Technology,9477S PP2A/C subunit (1D6) (mouse monoclonal)1:2000,Millipore,05-421 Flag M2 (mouse monoclonal)1:2000,Sigma,A8592-.2MG GST

(18) PIN1 and CDK1 cooperatively govern pVHL stability and suppressive functionsCell death and differentiationApril 1, 2023Chen J, Li M, Liu Y, Guan T, Yang X, Wen Y, Zhu Y, Xiao Z, Shen X, Zhang H, et al.dilution: 1:1000) antibody was purchased from Proteintech Group (IL, USA). Anti-CDK substrate antibody (9477 S, dilution: 1:500), anti-p-Akt (T308) antibody (13038 S, dilution: 1:1000) and anti-Akt (pan) antibody

(19) Phosphorylation of USP29 by CDK1 Governs TWIST1 Stability and Oncogenic FunctionsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)April 1, 2023Guan T, Li M, Song Y, Chen J, Tang J, Zhang C, Wen Y, Yang X, Huang L, Zhu Y, et al.(13116T, dilution: 1:1000), anti‐Vimentin (5741T, dilution: 1:1000) and anti‐CDK substrate antibody (9477S, dilution: 1:500) were purchased from CST (Cell Signaling Technology). Anti‐TWIST1 (ab50887, dilution:

(20) Tceal7 Regulates Skeletal Muscle Development through Its Interaction with Cdk1International journal of molecular sciencesMarch 27, 2023Xiong Z, Wang M, Wu J, Shi X.antibody (#9234, Cell Signaling Technology, Danvers, MA, USA), phospho-CDK Substrate Motif antibody (#9477, Cell Signaling Technology, Danvers, MA, USA), 4E-BP1 antibody (#9644, Cell Signaling Technology, Danvers,

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Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab ™ Rabbit mAb mix

Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab Rabbit mAb mix from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab Rabbit mAb mix from Cell Signaling Technology

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