CPT1A (D3B3) Rabbit mAb 12252S from Cell Signaling Technology

Product Specs
ItemCPT1A (D3B3) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number12252S
Quantity100 µl
ApplicationsW, W-S, IP
ReactivityH
ClonalityMonoclonal
TargetCPT1a
HostRabbit
NCBI Full Gene Namecarnitine palmitoyltransferase 1A
NCBI Gene AliasesCPT1, CPT1-L, L-CPT1
Research AreaMetabolism
IsotypeIgG
CloneD3B3
Molecular Weight88

Questions and Answers

Can this antibody be used to study CPT1A in cancer research, particularly in uveal melanoma?

Yes, the CPT1A (D3B3) Rabbit mAb has been utilized in studies investigating uveal melanoma, where it was shown that targeting fatty acid metabolism, including CPT1A, can suppress tumor growth. Specifically, elevated levels of CPT1A were associated with metabolic adaptations in uveal melanoma cells, making it a relevant target for therapeutic strategies ().

Is this antibody effective for detecting CPT1A in Western blotting applications?

The CPT1A (D3B3) Rabbit mAb has been successfully employed in Western blotting, as evidenced by its use in various studies to quantify CPT1A protein levels in different cell types, including cancer cells. It has been cited in multiple publications that detail its application in this technique (, ).

How does CPT1A expression relate to metabolic processes in cancer cells?

CPT1A plays a crucial role in fatty acid oxidation, which is essential for energy metabolism in cancer cells. Studies have indicated that inhibition of CPT1A can lead to reduced mitochondrial activity and promote anoikis in high-grade serous ovarian cancer cells, highlighting its importance in metabolic reprogramming during cancer progression ().

Are there any specific experimental conditions under which this antibody has been validated?

The CPT1A (D3B3) Rabbit mAb has been validated for use in immunoprecipitation and Western blotting under various experimental conditions, including in vitro studies with cancer cell lines and in vivo models. It has been cited in research that demonstrates its effectiveness in detecting CPT1A under conditions of metabolic stress and during cancer treatment responses (, ).

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Inhibiting TRIM21 neddylation rejuvenates oocyte quality in PCOS by regulating ubiquitination of CPT1AResearch (Wash D C)April 3, 2026Xinni Na, Jin-Chi Liu, YINAN TAN, LINGBO MENG, CUISHAN GUO, NA ZUO, WANLIN DAI, Ruiting Cong, BOWEN ZHANG, WANTING SHI, et al.12108-1-AP), anti-TRIM21 (Proteintech, cat# 67136-1-Ig), anti-CPT1A (Cell Signaling Technology, cat# 12252), anti- CPT1A (Cell Signaling Technology, cat# 97361), anti-CPT1A (Proteintech, cat# 66039-1-Ig), anti-CPT1A

(2) Etifoxine drives macrophage M2 polarization via Schwann cell-derived progesterone activation of PPARγ to accelerate peripheral nerve repairFrontiers in Cellular NeuroscienceMarch 23, 2026Chao Guo, Song Liuovernight at 4°C with the following primary antibodies: anti-PPARγ (CST, #2435), anti-CPT1A (CST, #12252), anti-PGC1α (CST, #2178), anti-BNIP3L (Selleck, F0469), anti-Parkin (Selleck, F0296), anti-NLRP3 (Selleck,

(3) Apigenin Suppresses Bladder Cancer via the SIRT6-NCOA2-PPARα AxisInternational Journal of Biological SciencesFebruary 26, 2026Ying Liu, Zhen-Duo Shi, Yun-Fei Wei, Si-Yuan Jiang, Harsh Patel, Qian-Zi Liu, Yang Dong, Yi-Fang Liu, Lin Hao, Shan Gao, et al.goat serum and incubated overnight at 4 °C with primary antibodies diluted in PBS: CPT1A (CST, USA, 12252), FASN (CST, USA, 3180). HRP-conjugated anti-rabbit secondary antibody (Abcam, UK, ab205718) was applied

(4) Transplantation of Soluble Epoxide Hydrolase Inhibitor-Treated Human Brown Adipocytes Promotes Adipose Tissue Activation in High-Fat-Diet-Fed Nude MiceInt J Mol SciJanuary 31, 2026Haoying Wu, Xinyun Xu, Jiangang Chen, Christophe Morisseau, Bruce D. Hammock, Yu-Hua Tseng, Ling Zhao"Novus Biologicals (Centennial, CO, USA)",NB400-144 Anti-CPT1A,"Cell Signaling Technology (Danvers, MA, USA)",12252S Anti-CPT1B,"Proteintech North America (Rosemont, IL, USA)",22170-1-AP Anti-ERK1/2,"Cell Signaling

(5) Putative Role of Fatty Acid Metabolic Therapy Using Ketogenic Diet and HIF-1α Inhibition in Hepatocellular Carcinoma: Evidence from an In Vitro StudyInt J Mol SciDecember 5, 2025Naoya Kimura, Yoshihiko Kitajima, Kazuki Higure, Shohei Matsufuji, Shota Ikeda, Shunsuke Furukawa, Kumpei Yukimoto, Tomokazu Tanaka, Hirokazu Noshiro13989-1-AP; Proteintech, Rosemont, IL, USA), CPT1A (carnitine palmitoyltransferase 1A; 1:1000; cat. no. 12252; Cell Signaling Technology), MCAD (medium-chain acyl-CoA dehydrogenase; 1:1000; cat. no. 55210-1-AP;

(6) Ei24 deficiency in brown adipocytes induces severe hypothermia under cold stress independent of UCP1 activityNat CommunDecember 1, 2025Su Bin Lee, Bui Thanh Thu, Tae Wook Nam, Yaechan Song, Jae Hoon Lee, Yangsik Jeong, Han-Woong LeeCell Signaling Technology) HSL (#4107, Cell Signaling Technology), FABP4 (#3544 T, Cell Signaling Technology), CPT1A (#12252S, Cell Signaling Technology), PPARγ (sc-7273, Santa Cruz Biotechnology), PLIN1 (#9349, Cell Signaling Technology

(7) Comparative Assessment of Synthetic and Algal‐Derived Astaxanthin Supplementation on Growth Traits, Lipid Homeostasis, and Liver Well‐Being in High‐Fat Diet‐Fed Trachinotus ovatusAquaculture NutritionOctober 29, 2025Yucai Guo, Haiqi Pu, Sihan Lin, Baoyang Chen, Anqi Chen, Mingyan Huai, Wei Zhao, Jin Niu(AMPK) (5831S), and phospho-AMPK (p AMPK) (2535S) antibodies, CPT1 (Carnitine palmotoyl transferase 1) (12252S), FAS (Fatty acid synthase) (4233S) and β-actin (4970S) were purchased from Cell Signaling Technology

(8) Development of a diagnostic model for MASLD and identification of daidzein as the potential drug using bioinformatics analysis and experimentsFrontiers in ImmunologyOctober 22, 2025Tao Wang, Hao Zhang, Kaixia Wang, Chunxue Liu, Nan Kong, Luocheng Zhou, Lihong Qu,Cell Signaling Technology; 3180S SCD1,Rabbit,Cell Signaling Technology; 2794 CPT1A,Rabbit,Cell Signaling Technology; 12252 CD36,Rabbit,Cell Signaling Technology; 28109S β-actin,Rabbit,ABGENT; AP14779b Antibodies used for western blot

(9) Rebamipide Induces Hair Regeneration Through EP4-Driven Lipid Metabolism RemodelingInternational journal of molecular sciencesOctober 18, 2025Feng C, Dong H, Jiang D, Gao Y, Gu X, Diao W, Zhou Y, Xu D, Li R, Wu L.50081, CST), anti-AMPKα (1:1000; 5832, CST), anti-Beclin-1 (1:1000; 3495, CST), anti-CPT1A (1:1000; 12252, CST), anti-ATGL (1:1000; 2439, CST), anti-FABP4 (1:2000; 2120, CST), anti-PPARα (1:1000; ab126285,

(10) HSP90 deficiency promotes cholesteryl ester accumulation in lipid droplets via endocytosis of low-density lipoproteinCommun BiolAugust 6, 2025Chengxin Ma, Xinmeng Che, Yan Liang, Jiacheng Li, Kuan Yu, Tong-Jin Zhao, Peng Li, Feng-Jung Chen70–80%. Antibodies and chemicals Antibodies against HSP90 (Cat# 4877), PNPLA2 (Cat# 2138), CPT1A (Cat# 12252), TOMM20 (Cat# 42406), COXIV (Cat# 4850), ATG7 (Cat# 8558T), ULK1 (Cat# 8054T), LC3 (Cat# 12741), FASN

(11) GSTP1 knockdown induces metabolic changes affecting energy production and lipid balance in pancreatic cancer cellsMol Cell OncolJune 17, 2025Jenna N. Duttenhefner, Katie M. ReindlSanta Cruz Biotechnology, while 4-hydroxynonenal (4-HNE, ab46545) was purchased from Abcam. Antibodies targeting CPT1A (12252S), GAPDH (97166S), β-actin (4970S), and horseradish peroxidase (HRP)-conjugated secondary antibodies

(12) Lipid exposure leads to metabolic dysfunction in fetal sheep cardiomyocytesPhysiol RepMay 26, 2025Natasha N. Chattergoon, Karthikeyan Bose, Samantha Louey, Sonnet S. Jonkerweight,Vendor,Catalog number,RRID CD36,75–85 kDa,Abcam,ab133625,AB_2716564 CPT1A,88 kDa,Cell Signaling Technology,12252,AB_2797857 CPT1B,88 kDa,Abcam,ab104662,AB_10712608 PPARA,42–45 kDa,Abcam,ab24509,AB_448110 Total

(13) Metabolic reprogramming by endothelial ANGPTL4 depletion protects against diabetic kidney diseaseBIORXIVMay 12, 2025Swayam Prakash Srivastava, Han Zhou, Rachel Shenoi, Myshal Morris, Begoña Lainez-Mas, Yuta Takagaki, Barani Kumar Rajendran, Ocean Setia, Binod Aryal, Keizo Kanasaki, et al.antibodies were purchased from Abcam (Cambridge, UK). Carnitine palmitoyl transferase 1a (CPT1a) (Cat:12252), rabbit polyclonal anti-E-cadherin antibody (24E10) (Cat:3195) and rabbit polyclonal PKM2 (D78A4)-XPCR

(14) Resistin upregulates fatty acid oxidation in synoviocytes of metabolic syndrome-associated knee osteoarthritis via CAP1/PKA/CREB to promote inflammation and catabolismArthritis Res TherApril 29, 2025Lu Ding, Jin-Yi Ren, Yi-Fan Huang, Jian-Zeng Zhang, Zi-Ran Bai, Yi Leng, Jun-Wei Tian, Jing Wei, Min-Li Jin, Guan Wang, et al.(Cell Signaling Technology, #2867), LDHA (Cell Signaling Technology, #3582), CPT1A (Cell Signaling Technology, #12252), CAP1 (Abcam, ab155079), TLR4 (WanleiBio, WL00196), CREB (Cell Signaling Technology, #9197), pCREBser133

(15) IDH status dictates oHSV mediated metabolic reprogramming affecting anti-tumor immunityNat CommunApril 24, 2025Upasana Sahu, Matthew P. Mullarkey, Sara A. Murphy, Joshua C. Anderson, Vasanta Putluri, Abu Hena Mostafa Kamal, Jun Hyoung Park, Tae Jin Lee, Alexander L. Ling, Benny A. Kaipparettu, et al.ThermoFisher Scientific, USA), GPX4 (Cat. No. 52455S, Cell Signaling Technology, USA), CPT1A (Cat. No. 12252S, Cell Signaling Technology, USA), LPCAT3 (Cat. No. 72964, Cell Signaling Technology, USA) and pan-p-PKC

(16) Metabolic Inhibition Induces Pyroptosis in Uveal MelanomaMolecular Cancer ResearchApril 1, 2025Scott D. Varney, Dan A. Erkes, Glenn L. Mersky, Manal U. Mustafa, Vivian Chua, Inna Chervoneva, Timothy J. Purwin, Emad Alnemri, Andrew E. Aplin(Cell Signaling Technology Cat# 9542, RRID:AB_2160739), anti-CPT1A (Cell Signaling Technology Cat# 12252, RRID:AB_2797857), anti-Vinculin (Santa Cruz Biotechnology Cat# sc-73614, RRID:AB_1131294), and anti-β-actin

(17) Biguanides antithetically regulate tumor properties by the dose-dependent mitochondrial reprogramming-driven c-Src pathwayCell Rep MedFebruary 18, 2025Jun Hyoung Park, Kwang Hwa Jung, Dongya Jia, Sukjin Yang, Kuldeep S. Attri, Songyeon Ahn, Divya Murthy, Tagari Samanta, Debasmita Dutta, Meron Ghidey, et al.,Cell Signaling Technology,Cat#2793S; RRID: AB_915794 Mouse monoclonal anti-CPT1A,Cell Signaling Technology,Cat#12252S; RRID: AB_2797857 Rabbit polyclonal anti-c-Myc,Cell Signaling Technology,Cat#9402S; RRID: AB_2151827 Rabbit

(18) MSCs with upregulated lipid metabolism block hematopoietic stem cell differentiation via exosomal CTP-1A in MDSStem cell research & therapyFebruary 7, 2025Yin C, Yan X, Ren J, Zhang C, Liu J, Wang Z, Liu J, Li W, Li X.specific antibodies (1:1000). Anti-CD63 Rabbit pAb (Wanleibio, # WL02549), CPT1A (D3B3) Rabbit mAb (CST, #12252). Flow cytometry Harvested cells were incubated with respective antibodies for 40 min at 4 °C after

(19) PPARα-mediated lipid metabolism reprogramming supports anti-EGFR therapy resistance in head and neck squamous cell carcinomaNat CommunFebruary 1, 2025Valentin Van den Bossche, Julie Vignau, Engy Vigneron, Isabella Rizzi, Hannah Zaryouh, An Wouters, Jérôme Ambroise, Steven Van Laere, Simon Beyaert, Raphaël Helaers, et al.Cell Signaling Technology; 1:1000), CD36 (#NB400-144, Novus Biologicals; 1:1000), CPT1A (clone D3B3, #12252, Cell Signaling Technology; 1:1000), phosphorylated (Tyr-1068; clone D7A5, #3777, Cell Signaling Technology;

(20) Targeting ketone body metabolism in mitigating gemcitabine resistanceJCI insightDecember 20, 2024Rohena-Rivera K, You S, Kim M, Billet S, Ten Hoeve J, Gonzales G, Huang C, Heard A, Chan KS, Bhowmick NA.antibodies were used: OXCT1 (HPA061425, Sigma-Aldrich), HMGCS2 (20940S, Cell Signaling Technology), CPT1A (12252S, Cell Signaling), PPARγ (sc-7273, Santa Cruz Biotechnology Inc.) OVOL1 (PA5-41480, Thermo Fisher Scientific),

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CPT1A (D3B3) Rabbit mAb

CPT1A (D3B3) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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CPT1A (D3B3) Rabbit mAb from Cell Signaling Technology

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