RBPSUH (D10A4) XP ® Rabbit mAb 5313S from Cell Signaling Technology

Product Specs
ItemRBPSUH (D10A4) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number5313S
Quantity100 µl
ApplicationsW, IHC-P, ChIP
ReactivityH, M, R, Mk
ClonalityMonoclonal
TargetRBPSUH
HostRabbit
NCBI Full Gene Namerecombination signal binding protein for immunoglobulin kappa J region
NCBI Gene AliasesAOS3, CBF-1, CBF1, IGKJRB, IGKJRB1, KBF2, RBP-J, RBP-J kappa, RBP-JK, RBPJK, RBPSUH, SUH, csl
Research AreaDevelopmental Biology
IsotypeIgG
CloneD10A4
Molecular Weight61

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Cell Signaling Technology

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(1) Nitrate Enhances Gastric Mucosa Defense and Repair Process in Ethanol-Induced Gastric Ulcer Rats via the Notch-Tff2 PathwayMedComm (2020)February 5, 2026Ying Liu, Xin Wen, Yuxuan Lin, Chunmei Zhang, Jinsong Wang, Guangyong Sun, Dong Zhang, Renhong Yan, Mo Chen, Songlin Wang, et al.mutated oligonucleotides. The probes were incubated with the GES‐1 cells extract and RBPJ antibody (5313; Cell Signaling Technology, USA) at room temperature for 50 min. To comprehend the specificity of the

(2) The notch-miR-188-5p-TIMP2/3 axis orchestrates exosome-driven pre-metastatic niche formation in colorectal cancerCancer Cell InternationalJanuary 12, 2026Yaochun Wang, Jingzhuo Song, Shaoran Song, Shuhang Zheng, Yuyao Li, Lingxiao Zhang, Bo Wang, Shuhong WangDiagenode). Immunoprecipitation was performed using 5 µg anti-RBP-J antibody (Cell Signaling Technology, #5313) or IgG control (Millipore). Precipitated DNA was analyzed by qPCR with primers flanking the miR-188-5p

(3) Defective Notch1 signaling in endothelial cells drives pathogenesis in a mouse model of Adams-Oliver syndromeJournal of Clinical InvestigationDecember 1, 2025Alyssa F. Solano, Kristina Preusse, Brittany Cain, Rebecca Hotz, Parthav Gavini, Zhenyu Yuan, Benjamin Bowen, Gabrielle Maco, Hope Neal, Ellen K. Gagliani, et al.room temperature, and then incubated with antibodies against RBPJ (1:1,000, Cell Signaling Technology, 5313) and β-actin (1:2,000, LI-COR, 926-42212) overnight at 4°C. The membrane was washed in PBS with 0.1%

(4) Disruption of Krox20-Notch1 signaling blocks meibomian gland development and homeostasis leading to dry eye diseaseNature CommunicationsNovember 23, 2025Yumeng Zhang, Edem Tchegnon, Elnaz Ghotbi, Zhiguo Chen, Stefanie L. Moye, Yi He, Renée M. McKay, Lu Q. Le(ab13970, Abcam, 1:100), Rabbit anti-HES1 (11988, Cell Signaling, 1:100), Rabbit anti-RBPSUH (RBPJ) (5313, Cell Signaling, 1:100) for 24 h at 4 °C. Subsequently, the slides were incubated in secondary antibody

(5) SEL1L confers reduced EMT and drug resistance to breast cancer cells by promoting Shh degradationiScienceNovember 21, 2025Hao Zhou, Jie Wang, Xu Wang, Zhaoji Guo, Yan Chen, Erli Gao, Jie Sun, Siying Zhou, Xinyi Guan, Yuxin Wu, et al.,Cell Signaling Technology,Cat#3608S; RRID: AB_2153354 RBPSUH (D10A4) XP® Rabbit mAb,Cell Signaling Technology,Cat#5313S; RRID: AB_2665555 YAP (D8H1X) XP Rabbit mAb,Cell Signaling Technology,Cat#14074S; RRID: AB_2650491 TAZ (E8E9G) Rabbit mAb

(6) Numb-exon3 and full length Numb equivalently alleviate cholestatic liver fibrosis by inhibiting ductular reactionScientific ReportsNovember 14, 2025Yan-nan Xu, Meng-yao Zong, Wen Xu, Shi-hao Zhang, Dan-yang Wang, Xin-rui Zheng, Fei-fei Xing, Jun-yi Zhan, Jia-mei Chen, Gao-feng Chen, et al.Sox9 (E-9, sc-166505) (Santa Cruz Biotechnology, Inc., CA, USA); rabbit polyclonal antibody to RBP-Jκ (5313; Cell Signaling Technology, Danvers, MA, USA); rabbit polyclonal antibodies to Hes1 (ab108937) and EpCam

(7) Effective in vivo binding energy landscape illustrates kinetic stability of RBPJ-DNA bindingNat CommunFebruary 1, 2025Duyen Huynh, Philipp Hoffmeister, Tobias Friedrich, Kefan Zhang, Marek Bartkuhn, Francesca Ferrante, Benedetto Daniele Giaimo, Rhett A. Kovall, Tilman Borggrefe, Franz Oswald, et al.desired antibody. We immunoprecipitated the chromatin with anti-RBPJ antibody (Cell Signaling Technology, 5313S) and after washing, we eluted the chromatin with Elution Buffer (50 mM Tris pH 8.0, 10 mM EDTA pH 8.0,

(8) Gualou Guizhi Granule inhibits microglia-mediated neuroinflammation to protect against neuronal apoptosis in vitro and in vivoFront ImmunolJanuary 9, 2025Xuezhen Li, Han Huang, Yanan Li, Yi Feng, Jinxuan Wang, Shuping Luo, Yaping Chen, Yuqin Zhang, Guohong Yan, Lihong Nan(SC-70586, SANTACRuZ, USA), Notch1 (10062-2-AP, Proteintech, China), HES1 (#11988, CST, USA), RBPSUH (#5313, CST, USA), NICD (ab83232, abcam, USA), and β-actin (66009-1-lg, Proteintech, China). The degree of

(9) Functional screening reveals genetic dependencies and diverging cell cycle control in atypical teratoid rhabdoid tumorsGenome BiologyDecember 2, 2024Daniel J. Merk, Foteini Tsiami, Sophie Hirsch, Bianca Walter, Lara A. Haeusser, Jens D. Maile, Aaron Stahl, Mohamed A. Jarboui, Anna Lechado-Terradas, Franziska Klose, et al.3590), RB-pSer608 (Cell Signaling, 8147), RB-pSer795 (Cell Signaling, 9301), RBPSUH (Cell Signaling, 5313), RPA2 p34 (Millipore, 04–1481), Survivin (Cell Signaling, 2802), TOPK (Cell Signaling, 4942), and TOPO

(10) Reciprocal suppression between TGFβ signaling and TNF stimulation finetunes the macrophage inflammatory responseFASEB journal : official publication of the Federation of American Societies for Experimental BiologyJuly 15, 2024Xia Y, Inoue K, Zheng T, Qin Y, Zhao B.magnetic beads (Themo Fisher) with 5 μg of the appropriate antibody overnight at 4°C. RBP-J antibody (#5313) was from Cell Signaling Technology. After overnight incubation, antibody-bound magnetic beads were

(11) Reciprocal suppression between TGFβ signaling and TNF stimulation finetunes the macrophage inflammatory responseThe FASEB JournalJuly 15, 2024Yuhan Xia, Kazuki Inoue, Ting Zheng, Yongli Qin, Baohong Zhaomagnetic beads (Themo Fisher) with 5 μg of the appropriate antibody overnight at 4°C. RBP-J antibody (#5313) was from Cell Signaling Technology. After overnight incubation, antibody-bound magnetic beads were

(12) Differentiation of intestinal stem cells toward goblet cells under systemic iron overload stress are associated with inhibition of Notch signaling pathway and ferroptosisRedox BiologyJune 1, 2024Jing Zhao, Wan Ma, Sisi Wang, Kang Zhang, Qingqing Xiong, Yunqin Li, Hong Yu, Huahua Du"WB, IF" Anti-Occludin,HuaBio,R1510-33,WB Anti-OLFM4,Cell Signaling Technology,39141,IF Anti-RBP-Jk,Cell Signaling Technology,5313,WB Anti-SGLT1,HuaBio,ER1916-51,WB Anti-SLC7A5,HuaBio,ER1916-84,WB Anti-SLC7A11,HuaBio,ER1905-55,WB Anti-TfR,Bioss,bs-0988R,WB Anti-Vil1,Proteintech,16488-1-AP,"WB, IF

(13) Comprehensive genomic features indicative for Notch responsivenessNucleic Acids ResearchMay 22, 2024Benedetto Daniele Giaimo, Tobias Friedrich, Francesca Ferrante, Marek Bartkuhn, Tilman Borggrefe#14111), H3K36me2 (abcam 176921), H3K36me3 (Cell Signaling #4909S), or RBPJ (Cell Signaling Technology 5313). ChIP experiments were analyzed on a StepOnePlus™ sequence detector system (Applied Biosystem) using

(14) Shared and distinct interactions of type 1 and type 2 Epstein-Barr Nuclear Antigen 2 with the human genomeBMC GenomicsMarch 12, 2024Kenyatta C. M. F. Viel, Sreeja Parameswaran, Omer A. Donmez, Carmy R. Forney, Matthew R. Hass, Cailing Yin, Sydney H. Jones, Hayley K. Prosser, Arame A. Diouf, Olivia E. Gittens, et al.sc-100974; EBF1, Santa Cruz sc-137065; EBNA2, [PE2] Abcam ab90543; JUNB, Active Motif 39549; RBPJ, CST 5313; and SPI1, CST 2266) was performed using antibodies against each, in duplicate per cell line, using

(15) Single-cell CRISPR screens in vivo map T cell fate regulomes in cancerNatureDecember 1, 2023Zhou P, Shi H, Huang H, Sun X, Yuan S, Chapman NM, Connelly JP, Lim SA, Saravia J, Kc A, et al.Tonbo Bioscience, APC–anti-RUNX3/CBFA3 (527327, IC3765A, 1:100) was from R&D Systems, and anti-RBPJ (D10A4, 5313 T), Alexa Fluor 647–anti-TCF-1 (C63D9, 6709, 1:100) and APC–anti-pS6 (S235/236) (D57.2.2E, 14733, 1:100)

(16) Context-dependent regulation of Notch signaling in glial development and tumorigenesisScience advancesNovember 10, 2023Guo R, Han D, Song X, Gao Y, Li Z, Li X, Yang Z, Xu Z.Pharmingen, 558774), rabbit anti-pSMAD1/5/9 (Cell Signaling, 13820), rabbit anti-RBPJ (Cell Signaling, 5313T), rabbit anti-S100B (Dako, Z0311), goat anti- SOX10 (R&D Systems, AB-442208), guinea pig anti-SOX10

(17) Jagged1 Acts as an RBP-J Target and Feedback Suppresses TNF-Mediated Inflammatory OsteoclastogenesisThe Journal of ImmunologyNovember 1, 2023Courtney Ng, Yongli Qin, Yuhan Xia, Xiaoyu Hu, Baohong Zhao(Cell Signaling Technology, 9006) for 6 hours at 4°C after incubation with anti-RBP-J antibody (Cell Signaling Technology, 5313, 1:20) or equivalent amount of normal rabbit IgG (Cell Signaling Technology, 2729) as the negative isotype

(18) ZFYVE28 mediates insulin resistance by promoting phosphorylated insulin receptor degradation via increasing late endosomes productionNature CommunicationsOctober 26, 2023Liang Yu, Mengchen Xu, Yupeng Yan, Shuchen Huang, Mengmeng Yuan, Bing Cui, Cheng Lv, Yu Zhang, Hongrui Wang, Xiaolei Jin, et al.9004) following standard procedures. The prepared chromatin was reacted with anti-RBPJ (1:50, CST, 5313) and anti-IgG (1:50, CST, 2729) at 4 °C overnight. The enrichment of the DNA template was analyzed by

(19) Notch Signaling Regulates Mouse Perivascular Adipose Tissue Function via Mitochondrial PathwaysGenesOctober 20, 2023Yang C, Yang X, Harrington A, Potts C, Kaija A, Ryzhova L, Liaw L.μg/mL PHB2,Cell Signaling,14805,0.05 mg/mL PINK1,Novus Biologicals,BC100-494,1 mg/mL RBPJ-k,Cell Signaling,5313,0.025 mg/mL UCP1,Cell Signaling,14670,0.4 mg/mL V5 tag,ThermoFisher,R960-25,0.204 μg/mL Antibodies.

(20) Rbpj Deficiency Disrupts Vascular Remodeling via Abnormal Apelin and Cdc42 (Cell Division Cycle 42) Activity in Brain Arteriovenous MalformationStrokeJune 1, 2023Adhicary S, Fanelli K, Nakisli S, Ward B, Pearce I, Nielsen CM.standard procedures with PVDF membranes immunoblotted against Rabbit anti-RBPJ (1:1000, Cell Signaling, #5313) and Rabbit anti-GAPDH (1:1000, Cell Signaling Technology, #5174). Migration and Immunolabeling in

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RBPSUH (D10A4) XP ® Rabbit mAb

RBPSUH (D10A4) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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RBPSUH (D10A4) XP ® Rabbit mAb from Cell Signaling Technology

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