IFN-gamma (D3H2) XP ® Rabbit mAb 8455S from Cell Signaling Technology

Product Specs
ItemIFN-gamma (D3H2) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number8455S
Quantity100 µl
ApplicationsW, IP, IF-IC, FC-FP
ReactivityH
ClonalityMonoclonal
TargetIFN-gamma
HostRabbit
NCBI Full Gene Nameinterferon gamma
NCBI Gene AliasesIFG, IFI, IMD69
Research AreaImmunology and Inflammation
IsotypeIgG
CloneD3H2
Molecular Weight17, 19, 23

Questions and Answers

Can this antibody be used to study the role of IFN-γ in cancer research?

Yes, the IFN-γ (D3H2) XP® Rabbit mAb has been utilized in various studies to investigate the role of IFN-γ in cancer. For instance, in the study by Feng et al. (2021), the antibody was employed to assess the expression levels of IFN-γ in gastric cancer cells, highlighting its relevance in understanding tumor microenvironments and immune responses.

What applications has this antibody been shown to work in?

The IFN-γ (D3H2) XP® Rabbit mAb has been shown to work in several applications, including Western blotting, flow cytometry, immunocytochemistry, immunofluorescence, and immunoprecipitation. This versatility allows researchers to utilize the antibody in various experimental setups to study IFN-γ expression and function.

Is there any evidence of this antibody being used in studies related to immune responses?

Yes, the antibody has been cited in studies examining immune responses. For example, in the research by Hu et al. (2021), the antibody was used to detect IFN-γ levels in bone marrow-derived dendritic cells, contributing to the understanding of adaptive immunity and the effects of nicotine on immune cell function.

What is the recommended dilution for using this antibody in immunocytochemistry?

The recommended dilution for the IFN-γ (D3H2) XP® Rabbit mAb in immunocytochemistry is 1:100, as indicated in the literature. This dilution has been effectively used in various studies to visualize IFN-γ expression in different cell types.

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Comparison and analysis of the immune landscape at the tumour invasion front in patients with pMMR/MSI-H and pMMR/MSS colorectal cancerInternational Journal of Colorectal DiseaseJanuary 14, 2026Miao Shen, Guoqun Chen, Fengli Cai, Yangye Ren, Yifan Zhang, Jiajun Shiantibodies: CD4 (25229, CST, MA, USA), CD8α (98941, CST, MA, USA), CK-Pan (4545, CST, MA, USA), IFN-γ (8455, CST, MA, USA), CD16 (24326, CST, MA, USA) and CD56 (99746, CST, MA, USA). For each cycle, sections

(2) CD8 + T‐Cell Deletion Suppressed the Development of Injury‐Induced Experimental Neointimal Hyperplasia in Mice With or Without Chronic StressThe FASEB JournalSeptember 30, 2025Jingyuan Jin, Meiling Piao, Xianji Piao, Shangzhi Shu, Longguo Zhao, Zhibo Wang, Xueling Yue, Jinshun Piao, Xianglan Jin, Lina Hu, et al.immunofluorescence using mouse monoclonal anti‐CD8a and rabbit monoclonal anti‐IFN‐γ (1:200, cat. no. #8455, Cell Signaling Technology, Beverly, MA) to identify the cell source of IFN‐γ in the carotid arteries

(3) Upregulation of the interferon-inducible antiviral gene RSAD2 in neuroendocrine prostate cancer via PVT1 exon 9 dependent and independent pathwaysJournal of Biological ChemistryApril 1, 2025Rachel E. Bonacci, Meghan McGill, Nu Thuy Anh Le, Murtaza Barkarar, Colin Finnegan, Maya Wilson, Oluwabusola Ajagbe, Chinedum C. Udekwu, Kathryn Gorski, Jyothi Manohar, et al.anti-RSAD2 or anti-interferon gamma antibody (Thermo Fisher Scientific, PA5-106337; Cell Signaling, 8455) was applied at a concentration of 1:500 in 5% BSA for 1 h at room temperature. Subsequently, Alexa

(4) Therapeutic potency and possible mechanism of Wuhu decoction underlying asthmatic progression via Th1/Th2 imbalanceJournal of Thoracic DiseaseJanuary 24, 2025Yan Hu, Mengqing Wang, Yinhe Luo, Jing Xie, Luojia Jiao, Wei Tang, Yijue Deng, Bing Yao(Danvers, USA) at a 1:1,000 dilution, including IL-4 (#12227), IL-10 (#12163), GATA3 (#5852), IFN-γ (#8455), IL-2 (#12239), T-bet (#13232) and β-Actin (#3700). After washed, the membranes were incubated with

(5) CD8 + T-cell deficiency protects mice from abdominal aortic aneurysm formation in response to calcium chloride 2Journal of HypertensionNovember 1, 2024Zhuo Lin, Mantong Zhao, Xian Zhang, Jinshun Piao, Xintong Zheng, Shangzhi Shu, Longguo Zhao, Meiping Zhang, Guo-Ping Shi, Yanna Lei, et al.California, USA), anti-MMP-9 (1 : 100, sc-393859, Santa Cruz) and rabbit anti-IFN-γ (1 : 100, cat. no. 8455, CST, Cell Signaling Technology Beverly, Massachusetts, USA), anti-CD68 (1 : 100, bs-0649R, Bioss, Woburn,

(6) Identification of colon adenocarcinoma necroptosis subtypes and tumor antigens for the development of mRNA vaccinesHeliyonJune 30, 2024Yuqi Luo, Caijie Lu, Yiwen Huang, Weihua Liao, Yaoxing Huang(1:2000, 17563-1-AP, Proteintech), anti-MLKL (1:1500, 21066-1-AP, Proteintech), anti–IFN–γ (1:1000, 8455, CST), anti-TNF-α (1:1000, ab1793, Abcam) and anti-GAPDH (1:15000, 60004-1-lg, Proteintech) overnight

(7) Effect of Granzyme K, FasL and Interferon-γ Expression in Placentas with PreeclampsiaBiomedicinesApril 11, 2024Martina Vukoja, Marina Ćurlin, Katarina Vukojević, Nevenka Jelić-Knezović, Anita Kolobarić, Martina Orlović Vlaho, Violeta ŠoljićUSA)" FasL,1:300,Rabbit,Cytoplasm,"Sigma Aldrich SAB4501532 (St. Louis, MI, USA)" IFN-γ,1:100,Rabbit,Cytoplasm,"Cell signalling 8455 (Cell Signaling, Boston, MA, USA)" Primary antibodies used for double immunofluorescence staining.

(8) Thymopentapeptide Affects T-Cell Subsets by Modulating the Flora of the Skin Surface to Alleviate PsoriasisDrug design, development and therapyJanuary 1, 2024Liu X, Xi R, Du X, Wang Y, Cheng L, Yan G, Lu H, Liu T, Li F.Wuhan, China); anti-CD4 rabbit pAb (GB11064; Servicebio, Wuhan, China); IFN-γ (D3H2) XP® rabbit mAb (#8455; Cell Signaling Technology, Shanghai, USA); anti-ROR gamma antibody (ab113434; Abcam, Cambridge, UK);

(9) Myeloid-specific knockout of SHP2 regulates PI3K/PLCγ signaling pathway to protect against early myocardial infarction injuryAging (Albany NY)September 30, 2023Menglin Sha, Hongxing Li, Bingyan Guo, Xiaoyong Geng1:1000), p-NF-κB (CellSignaling, 3303,1:1000), TNFα (CellSignaling, 11948, 1:1000), IFNγ (CellSignaling, 8455, 1:1000), IL-1β (CellSignaling, 12703, 1:1000), and GADPH (CellSignaling, 5174, 1:1000) were added,

(10) Resveratrol Enhances the Radiosensitivity by Inducing DNA Damage and Antitumor Immunity in a Glioblastoma Rat Model under 3 T MRI MonitoringJournal of OncologyOctober 13, 2022Liping Qian, Lihua Mao, Weixing Mo, Rong Wang, Yunlong Zhangab135372, Abcam, UK), anti-Foxp3 antibody (1 : 800, #12653, CST, USA), and anti-IFN-γ antibody (1 : 100, #8455, CST, USA) at 4°C for 12 h. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488, ab150077, Abcam, UK) was subsequently

(11) Weipiling decoction alleviates N-methyl-N-nitro-N′-nitrosoguanidine-induced gastric precancerous lesions via NF-κB signalling pathway inhibitionChinese MedicineSeptember 9, 2022Penghui Yang, Hongmei Yang, Hengli Zhou, Qiuyue Li, Sufen Wei, Qi Wang, Yan Yan, Yongqiang Liu, Huafeng Pan, Siyi Li3033S), IκB-α (CST, 4814S), phospho-IκB-α (CST, 2859S), CD4+ (CST, 93518S), interferon-gamma (IFN-γ, CST, 8455S), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, CST, 5174S) () were used for western blotting

(12) Regulation of the Proliferation of Diabetic Vascular Endothelial Cells by Degrading Endothelial Cell Functional Genes with QKI-7Contrast Media & Molecular ImagingJune 3, 2022Jinmei Xu, Qingsong Zhao, Xu Han, Zheqi Zhang, Jiahui Qu, Zhifeng ChengIL-1β-specific antibody was purchased from CST, No.: 12703. IFN-γ-specific antibody was purchased from CST, No.: 8455. HRP-labeled goat anti-rabbit and goat anti-mouse secondary antibodies were purchased from Quanshijin

(13) Autophagy-based unconventional secretion of HMGB1 in glioblastoma promotes chemosensitivity to temozolomide through macrophage M1-like polarizationJournal of experimental & clinical cancer research : CRFebruary 22, 2022Li Z, Fu WJ, Chen XQ, Wang S, Deng RS, Tang XP, Yang KD, Niu Q, Zhou H, Li QR, et al.1:100, Abcam), anti-HMGB1 (sc-56698, 1:100, Santa), anti-TNF-α (MAB610, 1: 200, NOVUS), anti-IFN-γ (8455S, 1: 200, CST), anti-IL-1β (12703S, 1: 200, CST), anti-IL-6 (ab9324, 1: 100, Abcam), anti-IL-8 (MAB208,

(14) Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitisMolecular Medicine ReportsJanuary 13, 2022Yangzi Jin, Aichun ZhangCell Signaling Technology, Inc.), IL-17 (cat. no. 13828, 1:2,000; Cell Signaling Technology, Inc.), IFN-γ (cat. no. 8455, 1:1,500; Cell Signaling Technology, Inc.) and β-actin (cat. no. ab8227; 1:1,000; Abcam) at 4°C overnight.

(15) SLFN11 captures cancer-immunity interactions associated with platinum sensitivity in high-grade serous ovarian cancerJCI insightSeptember 22, 2021Winkler C, King M, Berthe J, Ferraioli D, Garuti A, Grillo F, Rodriguez-Canales J, Ferrando L, Chopin N, Ray-Coquard I, et al.anti–p-STAT1 Tyr701 (CST, 9167 1/1000 for IB), anti–p-STAT5 (CST, 9359 1/1000 for IB), anti–IFN-γ (CST, 8455 1/1000 for IB), and anti-histone H3 (CST, 3638 1/2000 for IB). RNA sequencing and RT-PCR. For RNA sequencing

(16) Banxia xiexin decoction affects drug sensitivity in gastric cancer cells by regulating MGMT expression via IL‑6/JAK/STAT3‑mediated PD‑L1 activityInternational journal of molecular medicineAugust 1, 2021Feng X, Xue F, He G, Ni Q, Huang S.cat. no. 29261s), anti-STAT3 (1:1,000; cat. no. 9139s), anti-IFN receptor (IFNR; 1:1,000; cat. no. 8455s), anti-PD-L1 (1:1,000; cat. no. 13684s), anti-MGMT (1:1,000; cat. no. 86039s) and anti-GAPDH (1:1,000;

(17) Banxia Xiexin Decoction Inhibits the Expression of PD-L1 Through Multi-Target and Multi-Pathway Regulation of Major Oncogenes in Gastric CancerOncoTargets and TherapyMay 19, 2021Xuan Feng, Feng Xue, Guihua He, Suiping Huang, Qing NiEGFR (1:1,000; cat. no. 2085S), anti- STAT3 (1:1,000; cat. no. 9139s), anti- IFNR (1:1,000; cat. no. 8455s), antiPD-L1 (1:1,000; cat. no. 13684s), anti- TLR4 (1:1,000; cat. no. 14358S), anti-Na/K ATPase (1:1000,

(18) TNF‑α promotes insulin resistance in obstructive sleep apnea‑hypopnea syndromeExperimental and Therapeutic MedicineMarch 29, 2021Lin Zhao, Yang Liu, Xiangrong Wangab216995; dilution, 1:1,000; Abcam), IL‑6 (cat. no. ab233706; dilution, 1:1,000; Abcam), IFN‑γ (cat. no. 8455; dilution, 1:1,000; Cell Signaling Technology, Inc.), insulin receptor substrate 1 (IRS1; cat. no. ab40777;

(19) K48-Linked Ubiquitination Contributes to Nicotine-Augmented Bone Marrow-Derived Dendritic-Cell-Mediated Adaptive ImmunityVaccinesMarch 19, 2021Hu CF, Liao XY, Xu DD, Ruan YB, Gao FG.K63-linkage specific polyubiquitin (D7A11, #5621), β-actin (13E5, #4970), interferon-γ (IFN-γ) (D3H2, #8455), perforin (E7D8R, #62550), horseradish peroxidase (HRP) conjugate secondary antibody were bought from

(20) MUC1-C integrates activation of the IFN-γ pathway with suppression of the tumor immune microenvironment in triple-negative breast cancerJournal for ImmunoTherapy of CancerJanuary 24, 2021Nami Yamashita et al.(pSTAT1) (Y701) (#7167S, 1:1000 dilution; CST), anti-JAK1 (#3332S, 1:1000 dilution; CST), anti- IFN-γ (#8455S, 1:1000 dilution; CST), anti- IRF1 (#8478S, 1:1000 dilution; CST), anti- IDO1 (#86 630S, 1:1000 dilution;

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IFN-gamma (D3H2) XP ® Rabbit mAb

IFN-gamma (D3H2) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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IFN-gamma (D3H2) XP ® Rabbit mAb from Cell Signaling Technology

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