The Phospho-RB-S795 Polyclonal Antibody from MyBioSource.com is a Rabbit Polyclonal antibody to p105-Rb, pRB, and RB1. This antibody recognizes Human, Mouse, and Rat antigen. The Phospho-RB-S795 Polyclonal Antibody has been shown to work in the following applications: Immunofluorescence, and Western Blot.
Description
The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9)