MyBioSource.com's Mouse Anti Human High-Mobility Group Box 1 is a Mouse monoclonal antibody. The Mouse Anti Human High-Mobility Group Box 1 Antibody was generated using HMG1, and HMGB1 as the antigen. It reacts with Human. This antibody has been shown to work in applications such as: ELISA, Immunohistochemistry, and Western Blot.
Description
Introduction: High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins