Anti-Sheep RBC (red blood cells), (rabbit IgM) from MyBioSource.com

Yes, the Anti-Sheep RBC antibody has been utilized in studies examining phagocytosis. In a recent study, sheep red blood cells were sensitized with this antibody and subsequently used to investigate the phagocytic capacity of microglial cells in the context of Zika virus infection, demonstrating its applicability in such experimental setups (bortolin2025).

The use of rabbit IgM is significant because it provides a strong immune response against sheep red blood cells. IgM antibodies are known for their ability to agglutinate and activate complement pathways, which is crucial for lysing sensitized erythrocytes in experimental assays, thereby facilitating studies on immune responses and phagocytosis.

Yes, this antibody is specific to sheep red blood cells. Its specificity is supported by its use in experimental protocols where it effectively sensitizes sheep erythrocytes for subsequent lysis in the presence of complement, as demonstrated in the literature (bortolin2025).

It is recommended to incubate the sheep red blood cells with the Anti-Sheep RBC antibody at room temperature for 30 minutes with rotation, followed by centrifugation and washing steps. This protocol ensures optimal sensitization of the erythrocytes for subsequent assays involving phagocytosis and complement activation (bortolin2025).