Immunoprecipitation: ASPP1 Antibody [NB110-40683] - Detection of human ASPP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASPP1 antibody NB110-40683 (lot NB110-40683-2) used for IP at 6 ug per reaction. ASPP1 was also immunoprecipitated by a previous lot of this antibody (lot NB110-40683-1) and rabbit anti-ASPP1 antibody NB110-40684. Detection: Chemiluminescence with an exposure time of 3 minutes.