Fig 1: miR-153 inhibited the expression of ROCK1 and NFATc3 proteins in HPASMCs. (A) Western blotting showing the protein expression levels of ROCK1 and NFATc3. Quantification of the western blotting analysis for the protein expression of (B) ROCK1 and (C) NFATc3. β-actin was used as the internal control and the expression value of inhibitor control was set to 1. Data represent the mean ± standard deviation. n=3. **P<0.01. (D) miR-153 mimics attenuated the expression of NFATc3 proteins in HPASMCs and the miR-153 inhibitor promoted the expression of NFATc3 proteins in HPASMCs. (scale bar=100 µm). miR, microRNA; ROCK1, ρ-associated, coiled-coil-containing protein kinase 1; NFATc3, nuclear factor of activated T cells cytoplasmic 3; HPASMCs, human pulmonary artery smooth muscle cells.
Fig 2: miRNA-135a expression is inversely correlated to ROCK1 protein expression in human early gastric cancer tissue.(A) miRNA-135a expression was measured by real-time PCR analysis. The ROCK1 protein level was evaluated using western blot analysis and quantified using densitometric method. In 59 patients with early gastric cancer, expression of ROCK1 shows a significantly negative correlation with that of miRNA-135a (r = −0.697, p<0.001). (B) Western blot analysis shows that patients with down-regulated miRNA-135a expression have significantly higher ROCK1 protein expression in tumor tissues compared to expression in corresponding normal tissues (p<0.001).
Fig 3: Effect of apatinib on expression of proteins in the RhoA/Rho-associated coiled-coil domain-containing protein kinase signaling pathway. Apatinib upregulated expression of RhoA and ROCK II proteins but had no significant effect on ROCK I expression in all groups (n = 8 in each group). ∗P less than 0.05. ROCK, Rho-associated coiled-coil domain-containing protein kinase.
Fig 4: Comparison of protein expression in osteosarcoma and adjacent noncancerous bone tissues. Representative in situ hybridization images for miR-335 expression in (A) osteosarcoma tissues and (B) adjacent noncancerous bone tissues (original magnification, ×400). Compared with the adjacent noncancerous bone tissues, the expression of (C) Rock1 protein was significantly increased, while (D) miR-335 was significantly decreased in osteosarcoma tissues. miR, microRNA; Rock1, Rho-associated serine-threonine protein kinase 1.
Fig 5: CID16020046 ameliorates sepsis-induced renal injury by inhibiting the RhOA/ROCK pathway. The protein expression levels of GRP55, RhOA, ROCK1 and ROCK2 in kidney tissues of in different groups were detected via western blotting. *P<0.05 and ***P<0.001. CLP, cecal ligation/perforation; RhOA, ras homolog family member A; ROCK, Rho-associated protein kinase.
Supplier Page from Abcam for Anti-ROCK1 antibody [EPR638Y]