Rabbit monoclonal [R18-2] Anti-Rat IgG Fc ab125900 from Abcam

Product Specs
ItemRabbit monoclonal [R18-2] Anti-Rat IgG Fc
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab125900
ApplicationsELISA
Concentration1 mg/ml
Antibody TypePrimary Antibody
TargetIg gamma-1 chain C region
Research AreaImmunology & Infectious Disease
CloneR18-2
HostRabbit
IsotypeIgG
ClonalityMonoclonal
Conjugate/TagUnconjugated
Quantity100 µL

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Renal Tissue-Derived Exosomal miRNA-34a in Diabetic Nephropathy Induces Renal Tubular Cell Fibrosis by Promoting the Polarization of M1 MacrophagesIET NanobiotechnologyApril 17, 2024Shuai Zheng, Yi Zeng, Liqing Chu, Taiyang Gong, Sihong Li, Min Yangincubated overnight at 4°C. After rinsing with 0.01 mol/L PBS, the secondary antibody (ab150081, ab175783, ab125900, 1 : 1,000, Abcam) mixture was added to the sample and incubated for 2 hr at 4°C in the dark. After

(2) MSCs-Derived miR-150-5p-Expressing Exosomes Promote Skin Wound Healing by Activating PI3K/AKT Pathway through PTENInternational Journal of Stem CellsNovember 30, 2022Cheng Xiu, Huining Zheng, Manfei Jiang, Jiaxu Li, Yanhong Zhou, Lan Mu, Weisong LiuCells of each group were treated with 5 μg Ago2 antibodies for each RIP, with normal rabbit IgG (Abcam, ab125900) as the NC. Then co-precipitated RNAs were extracted and detected by RT-qPCR analysis. Wound healing

(3) TTP protects against acute liver failure by regulating CCL2 and CCL5 through m6A RNA methylationJCI insightDecember 8, 2021Xiao P, Li M, Zhou M, Zhao X, Wang C, Qiu J, Fang Q, Jiang H, Dong H, Zhou R.assay was performed as previously described (38). IgG was used as a negative control (Abcam, catalog ab125900). Primers used for RIP are shown in Supplemental Table 1. iCLIP-PCR. iCLIP-PCR was performed as previously

(4) Overexpression of ADAM9 decreases radiosensitivity of hepatocellular carcinoma cell by activating autophagyBioengineeredSeptember 16, 2021Lijin Zhu et al.(ab109012, 1:5000), antibodies under 4°C overnight. TBSA-diluted anti-rabbit secondary antibody (Abcam, ab125900, 1:1000) was used for further incubation. The electrochemiluminescence kit (Thermo, Waltham, MA, USA)

(5) Long non-coding RNA TUG1 knockdown prevents neurons from death to alleviate acute spinal cord injury via the microRNA-338/BIK axisBioengineeredSeptember 14, 2021Hongbo Wu, Yi Li, Xiaofeng Wang, Zhiwen Zhang, Yuliang HuangThe cell extracts were then ice-bathed with the primary antibodies to Ago2 (ab32381, Abcam) or IgG (ab125900 or ab181236, Abcam) coupled with A/G agarose beads. The particles were washed twice with RIPA buffer

(6) Circular RNA circ_0001287 inhibits the proliferation, metastasis, and radiosensitivity of non-small cell lung cancer cells by sponging microRNA miR-21 and up-regulating phosphatase and tensin homolog expressionBioengineeredJanuary 20, 2021Chuan-Cui Zhang, Yuhua Li, Xian-Zhen Feng, Dian-Bo Lithe PVDF membrane was rinsed with TBST solution and incubated with Goat Anti-Rabbit IgG H & L (Abcam, ab125900, 1:1000) for 1 h at room temperature. After the membrane was rinsed with TBST solution again, the protein

(7) SCARA5 suppresses the proliferation and migration, and promotes the apoptosis of human retinoblastoma cells by inhibiting the PI3K/AKT pathwayMolecular Medicine ReportsJanuary 13, 2021Jinwei Wang, Sha Wang, Lu Chen, Jia Tananti-rabbit IgG secondary antibody (1:20,000; cat. no. ab6721; Abcam) or anti-rat IgG (1:20,000; cat. no. ab125900; Abcam) for 2–3 h at room temperature; after which, the immunostained protein bands were detected using

(8) RETRACTED: miR-363 Alleviates Detrusor Fibrosis via the TGF-ÃŽÂ²1/Smad Signaling Pathway by Targeting Col1a2 in Rat Models of STZ-Induced T2DMMolecular Therapy - Nucleic AcidsDecember 4, 2020Xue-Feng Li, Shu-Hua Zhang, Gui-Feng Liu, Shao-Nan YuAbcam), positive control anti-β-actin antibody (ab179467, Abcam), or NC normal mouse IgG antibody (ab125900, Abcam). The samples were next incubated with proteinase K by shaking in order to detach the protein,

(9) The microRNA-130a-5p/RUNX2/STK32A network modulates tumor invasive and metastatic potential in non-small cell lung cancerBMC CancerDecember 1, 2020Fang Ma, Yangchun Xie, Yiyu Lei, Zengshuyu Kuang, Xianling Liuchromatin. Protein-DNA complexes were immunoprecipitated using RUNX2 antibody (ab23981, Abcam) or IgG (ab125900, Abcam) as a control. The immunoprecipitated DNA was purified and tested by qPCR. STK32A promoter fragment

(10) LINC01089 is a tumor-suppressive lncRNA in gastric cancer and it regulates miR-27a-3p/TET1 axisCancer Cell InternationalDecember 1, 2020Xufeng Guo, Ming Lithat, the PVDF membrane was rinsed with TBST solution, then incubated with secondary antibodies (Abcam, ab125900, 1: 2000, Cambridge, UK) for 1 h at room temperature. After the PVDF membrane was rinsed with TBST solution

(11) LncRNA MIR4435‑2HG inhibits the progression of osteoarthritis through miR‑510‑3p spongingExperimental and Therapeutic MedicineJune 5, 2020Yingli Liu, Yun Yang, Liangjia Ding, Yuqin Jia, Yuntao Jiprimary incubation, cells were incubated with a goat anti‑rabbit IgG (HRP‑conjugated) antibodies (1:5,000; ab125900, Abcam) at room temperature for 1 h. Cells were observed using a CX23 fluorescence microscope (magnification,

(12) LPS-Inducible lncRNA TMC3-AS1 Negatively Regulates the Expression of IL-10Frontiers in immunologyJanuary 1, 2020Ye M, Xie M, Zhu J, Wang C, Zhou R, Li X.(Life Technologies) and measured by real-time PCR. Anti-p65 (D14E12; CST) and immunoglobulin G (IgG) (ab125900; Abcam, Cambridge, MA, USA) were used for RIP. Chromatin Immunoprecipitation Chromatin immunoprecipitation

(13) Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesiclesNature communicationsNovember 9, 2017de Castro Martin IF, Fournier G, Sachse M, Pizarro-Cerda J, Risco C, Naffakh N.

(14) Simulation of heterogeneous molecular delivery in tumours using µCT reconstructions and MRI validationMicrovascular researchNovember 1, 2016Thomas Rea Wittenborn, Thomas Nielsen, Jesper Skovhus Thomsen, Michael Robert Horsman, Jens Vinge Nygaard

(15) Mechanism of irradiation-induced mammary cancer metastasis: A role for SAP-dependent Mkl1 signalingMolecular oncologyOctober 1, 2015Asparuhova MB, Secondini C, Rüegg C, Chiquet-Ehrismann R.HRP‐conjugated polymer ImmPRESS (MP‐7401, Vector Laboratories) was used preceded by R18‐2 anti‐rat IgGs (ab125900, Abcam) in the case of tenascin‐C detection. All slides were counterstained with Hematoxylin II and

(16) Tumor vasculature is regulated by FGF/FGFR signaling-mediated angiogenesis and bone marrow-derived cell recruitment: this mechanism is inhibited by SSR128129E, the first allosteric antagonist of FGFRsJournal of cellular physiologyJanuary 1, 2015Pierre Fons, Geneviève Gueguen-Dorbes, Jean-Pascal Herault, Fabien Geronimi, Joël Tuyaret, Dol Frédérique, Paul Schaeffer, Cécile Volle-Challier, Jean-Marc Herbert, Françoise Bono

(17) Tenascin-C is required for normal Wnt/ß-catenin signaling in the whisker follicle stem cell nicheMatrix biology : journal of the International Society for Matrix BiologyNovember 1, 2014Ismaïl Hendaoui, Richard P Tucker, Dominik Zingg, Sandrine Bichet, Johannes Schittny, Ruth Chiquet-Ehrismann

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Rabbit monoclonal [R18-2] Anti-Rat IgG Fc from Abcam

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Rabbit monoclonal [R18-2] Anti-Rat IgG Fc from Abcam

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