Fig 2: Knockdown of CBFB inhibited proliferation, invasion and lipid synthesis of metastatic OSCC cells. A/B, CCK8 (A) and Transwell (B) assays were used to analysis the effects of CBFB knockdown on proliferation and invasion of BHY and HSC3 cells. C Protein expression of FASN, SCD and ACACA in BHY and HSC3 cells with si-CBFB or si-NC transfection. D/E TC (D) and TG (E) levels in BHY and HSC3 cells upon CBFB knockdown. All of the experiments were performed with three-time independently. **P < 0.001, si-CBFB versus si-NC. TC, total cholesterol. TG, triglyceride

Fig 3: Validation of PLA on pre-B lymphoblasts. a Quantitative real-time RT-PCR and western blot analysis showed respectively mRNA and protein expression of RUNX1, ETV6 or ETV6-RUNX1 in Nalm6 and REH cells. All RT-PCR (left panel) were performed in triplicate and gene expression was normalized to ABL1 expression (error bars are S.D.) while western blot analyses (right panel) are representative images from the whole-cell lysates. b Technical controls demonstrate the specificity of PLA signals in Nalm6 cells and the proximity between two proteins (RUNX1 and CBFB). Nuclei were stained with DAPI. RUNX1 ab110035 antibodies were incubated alone (−) or with RUNX1 ab23980, CBFB ab133600 or ETV6 sc11382 antibodies. Each picture (upper panel) is representative of a typical cell staining observed in 5 fields randomly chosen. The quantification of the number of PLA dot per nucleus is presented with the mean values ± S.D

Fig 4: Prediction and expression correlation analysis of transcription factors for ACAT1, OXSM and VAPA. A, GIGGLE score of 20 predicted transcription factors. B, Pearson’s correlation analysis of RXRA, CBFB, JMJD1C, NCOR1, ACAT1, OXSM and VAPA expression based on TCGA database

Fig 5: Regulation of ACAT1, OXSM and VAPA expression by CBFB in metastatic OSCC cells. A Genome regions with enhanced H3K27ac signaling at ACAT1, OXSM and VAPA locus in metastatic OSCC cells (HN120Met, BHY and HSC3 cells) and primary OSCC cell (HN120Pri cell) were analyzed using GSE88976. B/C qRT-PCR and Western blotting were used to measure the mRNA (B) and protein (C) levels of CBFB in BHY and HSC3 cells transfected with si-NC or si-CBFB. D ChIP-qPCR was performed to detect the alterations in H3K27ac modification of enhancers of ACAT1, OXSM and VAPA after CBFB knockdown in BHY and HSC3 cells. E/F qRT-PCR and Western blotting were performed to detect the effects of CBFB knockdown on mRNA (E) and protein (F) expression of ACAT1, OXSM and VAPA in BHY and HSC3 cells. All of the experiments were performed with three-time independently. **P < 0.001, si-CBFB versus si-NC