MHC class I (W6/32) Antibody sc-32235 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemMHC class I (W6/32) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-32235
Quantity200 µg/ml
Reactivityhuman
ApplicationsWB, IP, IF, FCM
Conjugate/TagUnconjugated
FormatPurified
Antibody TypePrimary Antibodies
CloneW6/32
Hostmouse
TargetMHC class I
IsotypeIgG2a
NCBI Gene AliasesHLAB, HLA-JY3, AS, PSORS1, D6S204, HLAC, B-4901, HLAA, HLC-C, MHC
ClonalityMonoclonal
NCBI Full Gene Namemajor histocompatibility complex, class I, C, major histocompatibility complex, class I, B, major histocompatibility complex, class I, A

Questions and Answers

Can this antibody be used for flow cytometry applications?

Yes, the MHC class I (W6/32) antibody has been shown to be effective for flow cytometry applications, as evidenced by its use in studies where it was employed to stain cells for analysis of cell surface markers, including in the context of immune responses and tumor microenvironments ().

Is this antibody suitable for use in immunoprecipitation experiments?

The MHC class I (W6/32) antibody has been utilized in immunoprecipitation experiments, where it was used to isolate MHC class I molecules from cell lysates, demonstrating its effectiveness in capturing these proteins for further analysis ().

What specific human proteins does this antibody target?

This antibody targets human major histocompatibility complex class I molecules, specifically HLA-A, HLA-B, and HLA-C, which are critical for immune recognition and response to pathogens and tumors.

Has this antibody been referenced in studies related to cancer immunotherapy?

Yes, the MHC class I (W6/32) antibody has been referenced in studies exploring cancer immunotherapy, particularly in the context of enhancing T cell responses against tumor cells by facilitating the transfer of MHC-I molecules to cancer cells, thereby promoting CD8+ T lymphocyte cytotoxic activity ().

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) HIF regulates multiple translated endogenous retroviruses: Implications for cancer immunotherapyCellApril 3, 2025Qinqin Jiang, David A. Braun, Karl R. Clauser, Vijyendra Ramesh, Nitin H. Shirole, Joseph E. Duke-Cohan, Nancy Nabilsi, Nicholas J. Kramer, Cleo Forman, Isabelle E. Lippincott, et al.lysate supernatant was incubated with 10 μg of HLA-I antibody (Santa Cruz Biotechnology Clone W6/32, sc-32235) and coupled with 25 μL dry weight GammaBind Plus Sepharose beads (Cytiva 17088601) under rotary agitation

(2) Cytokine screening identifies TNF to potentially enhance immunogenicity of pediatric sarcomasFrontiers in ImmunologyDecember 11, 2024Hendrik Gassmann, Melanie Thiede, Jennifer Weiß, Emilie Biele, Luisa Flohé, Helena Lachermaier, Carolin Prexler, Valentina Evdokimova, Laszlo Radvanyi, Irfan Akhtar, et al.(RPN2232; Amersham) using the ChemiDoc XRS+ imaging instrument. The primary antibody against MHC-I (W6/32, sc-32235), was obtained from Santa Cruz Biotechnology. GAPDH (ab9485) was purchased from Abcam. 2.6 xCELLigence

(3) PROTAC-mediated degradation of HIV-1 Nef efficiently restores cell-surface CD4 and MHC-I expression and blocks HIV-1 replicationCell chemical biologyApril 18, 2024Emert-Sedlak LA, Tice CM, Shi H, Alvarado JJ, Shu ST, Reitz AB, Smithgall TE.250 uL flow buffer and stained with CD4-APC (BD Biosciences #555349) or MHC-I-PE (W6/32, Santa Cruz #sc-32235) for 45 min at room temperature in the dark. Cells were then washed 3 more times with flow buffer and

(4) Hsp90 inhibition leads to an increase in surface expression of multiple immunological receptors in cancer cellsFrontiers in molecular biosciencesJanuary 1, 2024Wickenberg M, Mercier R, Yap M, Walker J, Baker K, LaPointe P.HLA-C (Novus Biologicals, NBP2-50419 PE), a PE-conjugated W6/32 antibody (Santa Cruz Biotechnology, sc-32235) to detect MHC1, a PE-conjugated antibody to detect EGFR1 (Invitrogen, MA5-28544), a PE-conjugated CD119 antibody

(5) TCR ligand potency differentially impacts PD-1 inhibitory effects on diverse signaling pathwaysThe Journal of experimental medicineDecember 4, 2023Chan W, Cao YM, Zhao X, Schrom EC, Jia D, Song J, Sibener LV, Dong S, Fernandes RA, Bradfield CJ, et al.chamber at the indicated time point. For MHCI blocking experiments, mouse anti-human MHCI clone W6/32 (sc-32235; Santa Cruz Biotechnology) antibody was added to APC immediately before coculture time course with human

(6) Androgen receptor inhibition suppresses anti-tumor neutrophil response against bone metastatic prostate cancer via regulation of TβRI expressionCancer LettersNovember 7, 2023Massar Alsamraae, Diane Costanzo-Garvey, Benjamin A. Teply, Shawna Boyle, Gary Sommerville, Zachary T. Herbert, Colm Morrissey, Alicia J. Dafferner, Maher Y. Abdalla, Rachel W. Fallet, et al.Burlington MA), Catalase (#ab76024, Abcam, Boston MA), CuZnSOD/SOD1 (#ab51254, Abcam, Boston MA), GAPDH (#sc-32235, Santa Cruz Biotechnology, Dallas TX). The blot was washed in TBST prior to incubating with the secondary

(7) Androgen receptor is a determinant of melanoma targeted drug resistanceNature communicationsOctober 14, 2023Samarkina A, Youssef MK, Ostano P, Ghosh S, Ma M, Tassone B, Proust T, Chiorino G, Levesque MP, Goruppi S, et al.anti-PAI-1 (#19773; Novus Biologicals, 1:200), anti-Axl (#AF154, R&Dsystems, 1:500), anti-MHC class I (#sc-32235, Santa Cruz, 1:500), anti-CD8a (#550281, BD Biosciences, 1:1000), anti-granzyme B (#AF1865, R&Dsystems,

(8) The N-terminal Proline Hinge Motif Controls the Structure of Bovine Herpesvirus 1-encoded Inhibitor of the Transporter Associated with Antigen Processing Required for its Immunomodulatory FunctionJournal of molecular biologyMarch 1, 2023Graul M, Karska N, Wąchalska M, Krupa P, Ślusarz MJ, Lubocki M, Bieńkowska-Szewczyk K, Rodziewicz-Motowidło S, Sieradzan AK, Lipińska AD.

(9) Targeting of tumor cells by custom antigen transfer: a novel approach for immunotherapy of cancerFrontiers in oncologyJanuary 1, 2023Nesi I, Della Bella C, Taddei ML, Santi A, Pranzini E, Paoli P, D'Elios MM, Ramazzotti M, Genovese M, Caselli A, et al.Western Blot Detection were purchased from Bio-Rad. Anti-β actin (C4) (sc-47778), anti MHC-I (F3) (sc-32235) were purchased from Santa Cruz Biotechnology. Anti HLA-ABC (polyclonal) (PA5115364) was obtained from

(10) HLA-I immunopeptidome profiling of human cells infected with high-containment enveloped virusesSTAR protocolsDecember 16, 2022Weingarten-Gabbay S, Pearlman LR, Chen DY, Klaeger S, Taylor HB, Welch NL, Keskin DB, Carr SA, Abelin JG, Saeed M, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, HLA Class I Antibody W6/32 (200 μg/mL),Santa Cruz Biotechnology,Cat # sc32235 "FITC anti-human HLA-A,B,C Antibody (W6/32 clone)",BioLegend,Cat # 311403 ,, Bacterial and virus

(11) Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expressionNature communicationsJuly 6, 2022Lei X, Lin H, Wang J, Ou Z, Ruan Y, Sadagopan A, Chen W, Xie S, Chen B, Li Q, et al.cancer cells lysis, the target cells were preincubated with MHC-I blocking antibody w6/32 (Cat. No. sc-32235, Santa Cruz, 10 μg/mL) for 2 h at 37 °C before coculture28. The result was further verified by using

(12) Reversal of viral and epigenetic HLA class I repression in Merkel cell carcinomaThe Journal of clinical investigationJuly 1, 2022Lee PC, Klaeger S, Le PM, Korthauer K, Cheng J, Ananthapadmanabhan V, Frost TC, Stevens JD, Wong AY, Iorgulescu JB, et al.PE (BioLegend 311406), APC (BioLegend 311410), or Alexa Fluor 647 (AF647; Santa Cruz Biotechnology sc-32235 AF647), and HLA-DR–FITC (BioLegend 307604). Whole-exome sequencing and mutation calling. Genomic DNA library preparation

(13) Unannotated proteins expand the MHC-I-restricted immunopeptidome in cancerNature biotechnologyFebruary 1, 2022Ouspenskaia T, Law T, Clauser KR, Klaeger S, Sarkizova S, Aguet F, Li B, Christian E, Knisbacher BA, Le PM, et al.expressing B721.221 cells or 0.1 to 0.2g cancer cells were immunoprecipitated with W6/32 antibody (sc-32235, Santa Cruz) as described previously1,2. 10 mM iodoacetamide was added to the lysis buffer to alkylate

(14) Systematic Determination of TCR-Antigen and Peptide-MHC Binding Kinetics among Field Variants of a Theileria parva Polymorphic CTL EpitopeJournal of immunology (Baltimore, Md. : 1950)February 1, 2022Svitek N, Saya R, Zhang H, Nene V, Steinaa L.captures biotinylated heavy chains, and incubated for 3 h at 4°C. After washing, the W6/32 mAb (cat # SC-32235; Santa Cruz Biotechnology, TX), which binds to a monomorphic epitope on β2m, but only when it is incorporated

(15) Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistanceNature communicationsDecember 6, 2021Deng Y, Xia X, Zhao Y, Zhao Z, Martinez C, Yin W, Yao J, Hang Q, Wu W, Zhang J, et al.(1:500, Santa Cruz Biotechnology, sc-55582, RRID: AB_831547), MHC-I (1:500, Santa Cruz Biotechnology, sc-32235, RRID: AB_627934), B2M (1:1000, Cell Signaling Technology, 12851S, RRID: AB_2716551), phospho-STAT1

(16) Endosomal recycling tubule scission and integrin recycling involve the membrane curvature-supporting protein LITAFJournal of cell scienceAugust 1, 2021Wunderley L, Zhang L, Yarwood R, Qin W, Lowe M, Woodman P.1:2000 for western blotting (WB), 1:200 for IF]; anti-MHC class I (Santa Cruz Biotechnology; W6/32; Cat. Sc-32235; 1:200 for IF); anti-CD59 (Thermo Fisher Scientific; MEM43; Cat. MA-19133; batch 75162694; 1:200 for

(17) Profiling SARS-CoV-2 HLA-I peptidome reveals T cell epitopes from out-of-frame ORFsCellJuly 22, 2021Weingarten-Gabbay S, Klaeger S, Sarkizova S, Pearlman LR, Chen DY, Gallagher KME, Bauer MR, Taylor HB, Dunn WA, Tarr C, et al.(Millipore Sigma, GE17-0886-01) and 50 ul of an MHC class I antibody (W6/32) (Santa Cruz Biotechnology, sc-32235). The immune complexes were captured on the beads by incubating on a rotor at 4°C for 3hr in the BSL3

(18) Biochemical and functional characterization of mutant KRAS epitopes validates this oncoprotein for immunological targetingNature communicationsJuly 16, 2021Bear AS, Blanchard T, Cesare J, Ford MJ, Richman LP, Xu C, Baroja ML, McCuaig S, Costeas C, Gabunia K, et al.

(19) Hepatitis C virus modulates signal peptide peptidase to alter host protein processingProceedings of the National Academy of Sciences of the United States of AmericaJune 1, 2021Hirano J, Yoshio S, Sakai Y, Songling L, Suzuki T, Itoh Y, Zhang H, Chen DV, Haga S, Oomori H, et al.(MBL); EMR8-5), anti-human MHC class I mouse monoclonal antibody (W6/32) (Santa Cruz Biotechnology, sc-32235), anti-mouse CD8a rat monoclonal antibody (BioLegend, 53-6.7), and anti-mouse CD4 rat monoclonal antibody

(20) Optimized Liquid and Gas Phase Fractionation Increases HLA-Peptidome Coverage for Primary Cell and Tissue SamplesMolecular & cellular proteomics : MCPJanuary 1, 2021Klaeger S, Apffel A, Clauser KR, Sarkizova S, Oliveira G, Rachimi S, Le PM, Tarren A, Chea V, Abelin JG, et al.to 0.2 g from tumor tissue or tumor-derived cell lines were immunoprecipitated with W6/32 antibody (sc-32235; Santa Cruz) as described previously (8) (supplemental Table S1). Iodoacetamide (10 mM) was added to

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MHC class I (W6/32) Antibody

MHC class I (W6/32) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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MHC class I (W6/32) Antibody from Santa Cruz Biotechnology, Inc.

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