Fig 1: TFIIH recruitment to the chromatin fraction after DNA damage is compromised in XP-J cells.(A) TFIIH recruitment to damaged chromatin is only detectable in normal cells (input samples). (B–D) Immunoprecipitation was performed using the following antibodies: anti-XPB/p89 (B), XPD (C), p62 (D), and p52 (N-terminal) (E). TCL, total cell lysate; NucCyt, mixture of nuclear and cytoplasmic fractions; Chr, chromatin fraction. Antibodies used for immunodetection are indicated. The p52 (C) antibody recognizes the C-terminal domain of p52. PCNA serves as a loading control, and H2B is a histone protein localized in the chromatin fraction.