Fig 1: Binding of human condensin I complex to the TSS of active genes transcribed by RNAP2 and RNAP3.(a,b) ChIP-seq profiles of condensin I (using an antibody against NCAPG), RNAP2 (using 8WG16 monoclonal antibody, a) and RNAP3 (using monoclonal antibody against RPC32 subunit, b) in HeLa cells under the indicated conditions. M, cells arrested in prometaphase by nocodazole; Asy, asynchronous cells. Where indicated, the NCAPG subunit was depleted by siRNA treatment (Supplementary Fig. 8a). The upper boxes show the positions of protein-coding genes, and the lower boxes in b indicate the positions of tRNA genes (those within ORFs are shown in purple; others are shown in orange). The four sets of graphs in a and b show ChIP-seq profiles. The y axes of the ChIP-seq profiles indicate the normalized read intensity. Red indicates regions where enrichment determined by ChIP was statistically significant. (c) Distance of condensin I peaks from TSSs. More than 70% of NCAPG-binding sites are localized within ±5 kb of a TSS. (d) Level of condensin I binding during mitosis correlates with level of transcription during interphase. All RefSeq genes were ranked and divided into four equal groups based on their expression levels (high to low) in asynchronous cells, and averaged condensin I binding profiles around the TSS for each group were calculated. (e) Averaged mitotic NCAPG ChIP-seq profiles around tRNA genes. The profile for tRNA genes associated with RNAP3 in asynchronous cells is shown in purple.