Fig 2: Graphical summary of study. Ca2+, calcium; SR, sarcoplasmic reticulum; DHPR, dihydropyridine receptor; FKBP12, 12kDa FK506 binding protein; RyR1, ryanodine receptor1; CSQ1, calsequestrin1; SERCA1, sarcoplasmic reticulum calcium ATPase; SLN, sarcolipin; PLB, phospholamban; P-PLB, phosphorylated phospholamban; CaM, calmodulin; CaMK2, calmodulin kinase2; P-CaMK2, phosphorylated calmodulin kinase2; β-AR2, β-adrenergic receptor 2; cAMP-PKA, cyclic adenosine monophosphate -protein kinase A signaling pathway.

Fig 3: Protein overlap levels of DHPR and RyR1 in skeletal muscles during different periods. (A) Reticulate subcellular distribution of DHPR and RyR1 fluorescently labeled proteins in skeletal muscles. DAPI (blue) for nuclei, FITC (green) for DHPR, 647 (red) for RyR1. Scale bar = 20 µm. (B) Representative immunofluorescence of protein overlap between DHPR and RyR1 in three different types of muscle during different periods. Scale bar = 20 µm. (C) Box-plot representing protein overlap level of DHPR and RyR1. Boxes represent upper and lower quartiles, middle horizontal line represents median, hollow circle represents average, lines extending from upper and lower ends represent upper and lower edges, respectively, asterisks represent extreme outliers, and points represent individual sample values. n = 8. SOL, soleus muscle; EDL, extensor digitorum longus; GAS, gastrocnemius muscle; SA, summer active group; PRE, pre-hibernation group; LT, late torpor group; IBA, inter-bout arousal group; ET, early torpor group; POST, post-hibernation group. Different letters (such as a and b) indicate differences between period groups (P < 0.01), same letters (including a and ab) indicate no differences between period groups, and no letters indicate no differences among all six period groups.