IFN-γRα (GIR-94) Antibody sc-12755 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemIFN-γRα (GIR-94) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-12755
NCBI Gene AliasesIMD27B, IMD27A, CD119, Ifgr, Ifngr, IFN-gammaR, IFNGR, Nktar
Antibody TypePrimary Antibodies
Reactivitymouse, rat, human
CloneGIR-94
ApplicationsWB, IP, IF, FCM
Quantity200 µg/ml
FormatPurified
ClonalityMonoclonal
Conjugate/TagUnconjugated
Hostmouse
NCBI Full Gene Nameinterferon gamma receptor 1
TargetIFN-gammaRalpha
IsotypeIgG2b

Questions and Answers

Can this antibody be used for immunostaining in cell lines expressing IFN-γ receptor 1?

Yes, the IFN-γRα (GIR-94) antibody has been successfully used for immunostaining in cell lines such as Cos-7, which express IFN-γ receptor 1. In a study, cells were fixed and permeabilized before incubation with this antibody, demonstrating its effectiveness in detecting the receptor in various experimental setups ().

Is there any evidence that this antibody can be utilized in studies involving viral infections?

Yes, the IFN-γRα antibody has been employed in research examining the role of interferon gamma in inhibiting varicella-zoster virus replication. The antibody was used to confirm the presence of IFN-γ receptor in various cell lines, supporting its application in studies related to viral infections and immune responses ().

How has this antibody been applied in cancer research?

The IFN-γRα antibody has been utilized in studies investigating the immune microenvironment in high-grade canine mast cell tumors. It was used to assess the expression of IFN-γ in tumor samples, highlighting its relevance in understanding immune checkpoint interactions and potential therapeutic targets in cancer ().

What types of applications is this antibody suitable for?

The IFN-γRα antibody is suitable for various applications, including Western blotting, flow cytometry, immunofluorescence, and immunoprecipitation. Its versatility allows researchers to utilize it in multiple experimental contexts to study the interferon gamma receptor and its signaling pathways.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Clinical Implications of Immune Checkpoints and the RANK/RANK-L Signaling Pathway in High-Grade Canine Mast Cell TumorsAnimals : an open access journal from MDPIJune 6, 2023Talavera Guillén NC, Barboza de Nardi A, Noleto de Paiva F, Dias QC, Pinheiro Fantinatti A, Fávaro WJ.RANK-L",sc-52950,"Santa Cruz Biotechnology, USA",MCT skin: 1:50 "Rabbit, polyclonal anti-IFN-γ Rα",sc-12755,"Santa Cruz Biotechnology, USA",MCT skin: 1:50 Antibodies tested and antibodies dilution used for

(2) Inflammatory Cytokines Associated with Multiple Sclerosis Directly Induce Alterations of Neuronal Cytoarchitecture in Human NeuronsJournal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune PharmacologyJune 1, 2023Meyer-Arndt L, Kerkering J, Kuehl T, Infante AG, Paul F, Rosiewicz KS, Siffrin V, Alisch M.IL-10RB (clone F6, sc-271969, Santa Cruz), TNFR1 (clone H-5, sc-8436 Santa Cruz) or IFNGR1 (clone GIR 94, sc-12755, Santa Cruz) were added, and cells were incubated for 1 h at room temperature. Afterwards, matching

(3) N-glycosylation Regulates Intrinsic IFN-γ Resistance in Colorectal Cancer: Implications for ImmunotherapyGastroenterologyMarch 1, 2023Krug J, Rodrian G, Petter K, Yang H, Khoziainova S, Guo W, Bénard A, Merkel S, Gellert S, Maschauer S, et al.

(4) Chemical composite of indigenous whole grain scented joha rice varietal prevents type 2 diabetes in rats through ameliorating insulin sensitization by the IRS-1/AKT/PI3K signalling cascadeFood & functionNovember 14, 2022Choudhury P, Samanta SK, Bhattacharjee S, Sarma H, Devi R.

(5) Role of C5a and C5aR in doxorubicin-induced cardiomyocyte senescenceExperimental and therapeutic medicineOctober 1, 2021Wen Y, Shen F, Wu H.factor-binding protein 3 (IGFBP3; 1:1,000; cat. no. ab220429; Abcam), anti-IFN-γ (1:1,000; cat. no. sc-12755; Santa Cruz Biotechnology, Inc.), anti-TNF-α (1:1,000; cat. no. sc-12744; Santa Cruz Biotechnology, Inc

(6) Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and DegradationCancer discoveryJuly 1, 2021Du W, Hua F, Li X, Zhang J, Li S, Wang W, Zhou J, Wang W, Liao P, Yan Y, et al.(3% BSA and 0.01% saponin in PBS) for 30 minutes at room temperature. The IFNGR1 antibody (GIR-94) (sc-12755, Santa Cruz), LAMP1 antibody (D2D11) (9091, Cell Signaling Technology), or AP3D1 antibody (16454–1-AP,

(7) Role of CD4+ T Helper Cells in the Development of BAC-Induced Dry Eye Syndrome in MiceInvestigative ophthalmology & visual scienceJanuary 4, 2021Ouyang W, Wu Y, Lin X, Wang S, Yang Y, Tang L, Liu Z, Wu J, Huang C, Zhou Y, et al.µg/mL, catalog no. ab79056; Abcam) and monoclonal mouse anti-IFN-γ antibody (1:50, 4 µg/mL, catalog no. sc-12755; Santa Cruz Biotechnology, Dallas, TX, USA) diluted with 1% BSA overnight at 4°C. After they were washed

(8) Covalently Immobilizing Interferon-γ Drives Filopodia Production through Specific Receptor-Ligand Interactions Independently of Canonical Downstream SignalingBioconjugate chemistryMay 20, 2020Christie SM, Ham TR, Gilmore GT, Toth PD, Leipzig ND, Smith AW.anti-STAT1p (Tyr701, Invitrogen, 44–376G, 1:300 dilution), mouse anti-IFNγR1 (Santa Cruz Biotechnology, sc-12755, 1:400 dilution), and mouse anti-vinculin (7F9, Santa Cruz Biotechnology, sc-73614, 1:300 dilution).

(9) EGFR inhibition triggers an adaptive response by co-opting antiviral signaling pathways in lung cancerNature cancerApril 1, 2020Gong K, Guo G, Panchani N, Bender ME, Gerber DE, Minna JD, Fattah F, Gao B, Peyton M, Kernstine K, et al.IκBα (4814) antibodies were from Cell Signaling Technology (Danvers, MA); IFNAR1 (sc-7391), IFNGR1 (sc-12755), TNFR1 (sc-8346), and β-Actin (sc-47778) were from Santa Cruz Biotechnology (Dallas, TX); TRIM32 (Mab6515)

(10) Interferon Gamma Inhibits Varicella-Zoster Virus Replication in a Cell Line-Dependent MannerJournal of virologyJune 15, 2019Shakya AK, O'Callaghan DJ, Kim SK.extracts were used for Western blot analyses with the anti-human IFN-␥R␣ monoclonal antibody (MAb) (sc-12755; Santa Cruz Biotechnology, Santa Cruz, CA) and anti-␤-actin pAb. Molecular mass standards were from

(11) Helicobacter pylori Depletes Cholesterol in Gastric Glands to Prevent Interferon Gamma Signaling and Escape the Inflammatory ResponseGastroenterologyApril 1, 2018Morey P, Pfannkuch L, Pang E, Boccellato F, Sigal M, Imai-Matsushima A, Dyer V, Koch M, Mollenkopf HJ, Schlaermann P, et al.

(12) Swiprosin-1 deficiency impairs macrophage immune response of septic miceJCI insightFebruary 8, 2018Zhang S, Tu Y, Sun YM, Li Y, Wang RM, Cao Y, Li L, Zhang LC, Wang ZB.(rabbit pAb, sc-7606), P65 (rabbit pAb, sc-372), HLA-DRα (mouse mAb, sc55593), and IFN-γRα (mouse mAb, sc12755) antibodies were from Santa Cruz Biotechnology Inc. Swiprosin-1/ EFHD2 (goat pAb, ab24368), STAT1 (mouse

(13) The novel IFNGR1 mutation 774del4 produces a truncated form of interferon-gamma receptor 1 and has a dominant-negative effect on interferon-gamma signal transductionJournal of medical geneticsAugust 1, 2007Okada S, Ishikawa N, Shirao K, Kawaguchi H, Tsumura M, Ohno Y, Yasunaga S, Ohtsubo M, Takihara Y, Kobayashi M.

(14) Interferon (IFN)-gamma , tumor necrosis factor-alpha , interleukin-6, and IFN-gamma receptor 1 are the major immunological determinants associated with post-kala azar dermal leishmaniasisThe Journal of infectious diseasesOctober 1, 2006Ansari NA, Ramesh V, Salotra P.

(15) Measles virus suppresses interferon-alpha signaling pathway: suppression of Jak1 phosphorylation and association of viral accessory proteins, C and V, with interferon-alpha receptor complexVirologyFebruary 1, 2003Yokota S, Saito H, Kubota T, Yokosawa N, Amano K, Fujii N.

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IFN-γRα (GIR-94) Antibody

IFN-γRα (GIR-94) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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IFN-γRα (GIR-94) Antibody from Santa Cruz Biotechnology, Inc.

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