Fig 2: TCF12 mRNA expression level is increased in PC and associated with poor prognosis. (A) Gene Expression Profiling Interactive Analysis revealed that the mRNA expression of TCF12 in PC tumor tissues was higher compared with that in normal tissues. (B) Patients with PC and high TCF12 expression had shorter overall survival time. (C) The results from the Encyclopedia of RNA Interactomes website showed that TCF12 expression level was positively correlated with the expression of MAGT1 in tumor tissues from patients with PC. (D) Protein and (E) mRNA expression levels of TCF12 in PC cells, and human normal HPDE6c7 pancreatic ductal epithelial cell line were analyzed using western blot analysis and reverse transcription-quantitative PCR. *P<0.05 vs. normal tissue. **P<0.01, ***P<0.001 vs. HPDE6c7 group. TCF12, transcription factor 12; PC, pancreatic carcinoma; FC, fold change; T, tumor tissue; N, normal tissue; PAAD, pancreatic adenocarcinoma.

Fig 3: TCF12 promotes the proliferation, migration and invasion of pancreatic carcinoma cells by regulating the expression of MAGT1. (A) Cell Counting Kit-8 assay was used to determine the cell viability in cells transfected with si-NC, si-TCF12-2, si-TCF12-2+pcDNA3.1-NC and si-TCF12-2+pcDNA3.1-MAGT1-2 at different time points (24, 48 and 72 h. (B) Protein and mRNA expression levels of Ki67, and PCNA in BxPC-3 cells were decreased in cells transfected with si-TCF12-2 compared with that in cells transfected with si-NC group. Overexpression of MAGT1 reversed the protein expression level of Ki67 and PCNA. GAPDH served as the loading control. (C) The BxPC-3 cells transfected with si-TCF12-2 had decreased migration and invasion compared with that in cells transfected with si-NC. Overexpression of MAGT1 reversed the decreased migration and invasion. (D) Protein and mRNA expression level of MMP2, and MMP9 was significantly decreased in the BxPC-3 cells transfected with si-TCF12-2 compared with that in cells transfected with si-NC. The expression level of MMP2 and MMP9 were partly increased when co-transfected with pcDNA3.1-MAGT1, and si-TCF12-2. **P<0.01, ***P<0.001 vs. si-NC group; #P<0.05, ###P<0.001 vs. si-TCF12-2 + pcDNA3.1-NC group. NC, negative control; TCF12, transcription factor 12; MMP2, matrix metallopeptidase 2; si, small interfering.