Fig 1: Recruitment and phosphorylation of Pol II at Irf1gene and occupancy of TFIIH, p-TEFb, and Mediator components at the Irf1 TSS. (A) Schematic representation of Pol II, GTFs, and the Mediator complex at a GAS-driven gene promoter. Components of the Mediator, TFIIH, and p-TEFb complexes analyzed by ChIP are indicated. (B–L) BMDMs from WT and Stat1β/β mice were stimulated with IFNγ for the times indicated or left untreated (0 h). Association of Pol II, S5pPol II, and S2pPol II with the Irf1 promoter around the TSS (B–D) and of S2pPol II within the Irf1 gene body (E). Association of ERCC3 (F) and CDK9 (G) at the Irf1 TSS and of MED18 (H), MED4 (I), MED24 (J), MED26 (K), and MED1 (L) at the Irf1 GAS. Data were normalized to the input control. Mean values ± SE from two (H) or three (all others) independent experiments are shown; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.