Cya A (3D1) Antibody sc-13582 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemCya A (3D1) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-13582
Antibody TypePrimary Antibodies
ApplicationsWB, IP
ReactivityB. pertussis
Clone3D1
Quantity200 µg/ml
ClonalityMonoclonal
Conjugate/TagUnconjugated
Hostmouse
FormatPurified
TargetCya A
IsotypeIgG1

Questions and Answers

What applications has the Cya A (3D1) antibody been used for in scientific research?

The Cya A (3D1) antibody has been shown to work effectively in Western Blot applications, as evidenced by its use in various studies, including those investigating the role of Bordetella pertussis in stress responses and toxin release ().

Can this antibody be used to study Bordetella pertussis specifically?

Yes, the Cya A (3D1) antibody is specific for the adenylate cyclase toxin (CyaA) produced by Bordetella pertussis, making it suitable for research focused on this pathogen and its virulence factors ().

How has the Cya A (3D1) antibody been utilized in the context of Escherichia coli research?

The Cya A (3D1) antibody has been utilized in studies involving Escherichia coli, particularly in the context of the stringent response and the role of proteins like YtfK and SpoT in regulating (p)ppGpp accumulation, which is critical for bacterial stress responses ().

What is the reactivity profile of the Cya A (3D1) antibody?

The Cya A (3D1) antibody is reactive to bacteria and archaea, specifically targeting the CyaA protein in Bordetella species, which is relevant for studies on bacterial physiology and pathogenesis ().

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) The Chlamydia trachomatis -secreted effector protein CT181 binds to Mcl-1 and prolongs neutrophil survivalmBioJune 10, 2026Robert Faris, Rebecca Koch, Paige McCaslin, Naveen Challagundla, Brianna Steiert, Shelby E. Andersen, Alix McCullough, Parker Smith, C. A. Jabeena, Peter Yau, et al.running buffer. Proteins were transferred to a PVDF membrane and probed using anti-CyaA (Santa Cruz Cat# sc-13582), anti-BlaM (QED BioScience Cat# 15720), GSK-3β-Tag (Cell Signaling Cat# 9325S), or Phospho-GSK-3-beta

(2) Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule SensitivityAdvanced ScienceApril 10, 2026Marilyne Davi, Daniel Ladantmembrane (Millipore), incubated with the anti‐cyaA monoclonal antibody 3D1 [41] (Santa Cruz Biotechnology; sc‐13582) then with a horseradish peroxidase‐conjugated goat anti‐mouse secondary antibody (Santa Cruz Biotechnology,

(3) Control of cell division by an Acinetobacter baumannii protein with a novel nucleotidyl-cyclase-like foldbioRxivApril 7, 2026Andrew Farinha, Mark W Soo, George Minasov, Nicole L Inniss, Ludmilla Shuvalova, Vimisha Dharamdasani, Jessica Finkler, Oliver Stearns, Trisha Shenoy, Christopher Kim, et al.goat anti-rabbit IgG, HRP (Invitrogen 65-6120, 1:5000); or primary mouse CyaA antibody 3D1 (Santa Cruz Biotechnology SC-13582, 1:200) followed by secondary rabbit anti-mouse IgG, HRP (A27025, 1:5000). Blots were developed with

(4) Functional and intricate interaction network connecting Helicobacter pylori Cag type 4 secretion system surface proteins with outer membrane proteins HopQ and HopZMicrolifeOctober 6, 2025Felix Metz, Johanna Beilmann, Simon H Bats, Andreas Latoscha, Gregor Witte, Remco T A Megens, Karl-Peter Hopfner, Kaisa Thorell, Wolfgang Fischer, Laurent Terradot, et al.(αT18-antibody obtained from Santa Cruz Biotechnology, mouse monoclonal antibody 3D1 against B. pertussis CyaA, #sc-13582) fusion fragments of B. pertussis adenylate cyclase Cya. The latter two antisera also detect the T25

(5) A Retropepsin-Like Bacterial Protease Regulates Ribosome Modification and Polypeptide ProductionJournal of Biological ChemistryFebruary 18, 2025Richard H. Little, Govind Chandra, Gerhard Saalbach, Carlo Martins, Catriona M.A. Thompson, Jacob G. MaloneProtein was subsequently detected with 1/5000 Anti-CyaA primary antibody (Santa Cruz Biotechnology, # SC-13582) and goat anti-mouse secondary antibody (Millipore # 12–349) in 1x PBS pH 7.4, 0.01% Tween 20, 1% milk

(6) Characterization of DNA Processing Protein A (DprA) of the Radiation-Resistant Bacterium Deinococcus radioduransMicrobiology spectrumDecember 21, 2022Sharma DK, Misra HS, Soni I, Rajpurohit YS.working protocols were used, as described previously (51). The antibody against the T18 (catalog number SC-13582) domain of the CyaA protein of Bordetella pertussis was procured from Santa Cruz Biotechnology, Inc.,

(7) Identification of a Family of Vibrio Type III Secretion System Effectors That Contain a Conserved Serine/Threonine Kinase DomainmSphereAugust 25, 2021Plaza N, Urrutia IM, Garcia K, Waldor MK, Blondel CJ.the following dilutions: anti-CyaA (mouse monoclonal, 1:2,000; Santa Cruz Biotechnology catalog no. sc-13582), anti-RpoB (rabbit monoclonal, 1:2,000; Abcam catalog no. ab191598), anti-mouse IgG conjugated to horseradish

(8) The cyanobacterial taxis protein HmpF regulates type IV pilus activity in response to lightProceedings of the National Academy of Sciences of the United States of AmericaMarch 23, 2021Harwood TV, Zuniga EG, Kweon H, Risser DD.

(9) Novel Template Plasmids pCyaA'-Kan and pCyaA'-Cam for Generation of Unmarked Chromosomal cyaA' Translational Fusion to T3SS Effectors in SalmonellaMicroorganismsFebruary 25, 2021Fernández PA, Zabner M, Ortega J, Morgado C, Amaya F, Vera G, Rubilar C, Salas B, Cuevas V, Valenzuela C, et al.

(10) PprA Protein Inhibits DNA Strand Exchange and ATP Hydrolysis of Deinococcus RecA and Regulates the Recombination in Gamma-Irradiated CellsFrontiers in cell and developmental biologyJanuary 1, 2021Rajpurohit YS, Sharma DK, Misra HS.molecular biology techniques were used as described (Green and Sambrook, 2012). Antibodies against the T18 (SC-13582) and T25 (SC-33620) domains of CyaA of Bordetella pertussis were procured commercially (Santa Cruz Biotechnology, Inc

(11) PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthusNature communicationsOctober 7, 2020Treuner-Lange A, Chang YW, Glatter T, Herfurth M, Lindow S, Chreifi G, Jensen GJ, Søgaard-Andersen L.were used to detect GFP-tagged proteins (Roche; 11814460001) and CyaA-tagged proteins (Santa Cruz, sc-13582). As secondary antibodies, goat anti-rabbit immunoglobulin G peroxidase conjugate (Sigma-Aldrich, A8275)

(12) Structural basis for effector protein recognition by the Dot/Icm Type IVB coupling protein complexNature communicationsMay 26, 2020Kim H, Kubori T, Yamazaki K, Kwak MJ, Park SY, Nagai H, Vogel JP, Oh BH.each Legionella strain expressing a CyaA-tagged effector were used. Anti-CyaA mouse monoclonal IgG (sc-13582, Santa Cruz Biotechnology, Inc.) diluted to 1:3000 and goat anti-mouse IgG (#31430, Invitrogen) conjugated

(13) Deciphering the specific interaction between the acyl carrier protein IacP and the T3SS-major hydrophobic translocator SipB from SalmonellaFEBS lettersJanuary 1, 2020Canestrari MJ, Serrano B, Bartoli J, Prima V, Bornet O, Puppo R, Bouveret E, Guerlesquin F, Viala JP.

(14) YtfK activates the stringent response by triggering the alarmone synthetase SpoT in Escherichia coliNature communicationsDecember 17, 2019Germain E, Guiraud P, Byrne D, Douzi B, Djendli M, Maisonneuve E.buffer for 1 h (PBST + 5% milk). The first anti-cyaA antibody has been diluted 1/5.000 (Santa Cruz, ref: sc-13582) in PBST milk and incubated for 1 h at room temperaure. The secondary antibody (Sigma, ref: A4416) has

(15) Synthetic hydrophobic peptides derived from MgtR weaken Salmonella pathogenicity and work with a different mode of action than endogenously produced peptidesScientific reportsOctober 24, 2019Olvera MR, Garai P, Mongin G, Vivès E, Gannoun-Zaki L, Blanc-Potard AB.antibody (Tebubio) was used at 1:5000 dilution, whereas mouse anti-T18 antibody (monoclonal antibody sc-13582, Santa-Cruz) was used at 1:1000 dilution. Rabbit anti-OmpF and anti-OmpC antibodies were used at 1:1000.

(16) Interaction of ArmZ with the DNA-binding domain of MexZ induces expression of mexXY multidrug efflux pump genes and antimicrobial resistance in Pseudomonas aeruginosaAntimicrobial agents and chemotherapySeptember 9, 2019Kawalek A, Modrzejewska M, Zieniuk B, Bartosik AA, Jagura-Burdzy G.Western blot analysis of CyaT18-fused proteins was performed by the use of mouse anti-CyaA antibodies (3D1, catalog no. SC-13582; Santa Cruz Biotechnology). For Western blot analysis, cell pellets were resuspended in loading buffer

(17) N-terminal domain of DivIVA contributes to its dimerization and interaction with genome segregation proteins in a radioresistant bacterium Deinococcus radioduransInternational journal of biological macromoleculesMay 1, 2019Chaudhary R, Gupta A, Kota S, Misra HS.

(18) ParA proteins of secondary genome elements cross-talk and regulate radioresistance through genome copy number reduction in Deinococcus radioduransThe Biochemical journalMarch 15, 2019Kumar Maurya G, Kota S, Kumar NN, Tewari R, Misra HS.

(19) Role of leucine zipper-like motifs in the oligomerization of Pseudomonas putida phasinsBiochimica et biophysica acta. General subjectsFebruary 1, 2019Tarazona NA, Maestro B, Revelles O, Sanz JM, Prieto MA.

(20) Structure of the type VI secretion system TssK-TssF-TssG baseplate subcomplex revealed by cryo-electron microscopyNature communicationsDecember 19, 2018Park YJ, Lacourse KD, Cambillau C, DiMaio F, Mougous JD, Veesler D.assembly. Western blotting was performed using a mouse anti-CyaA (1:5000, Santa Cruz Biotechnologies #: SC-13582, Lot: C2715) and detected with an anti-mouse horseradish peroxidase-conjugated antibodies (1:5000, Sigma).

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Cya A (3D1) Antibody

Cya A (3D1) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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Cya A (3D1) Antibody from Santa Cruz Biotechnology, Inc.

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