MYH3 (F1.652) Antibody sc-53091 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemMYH3 (F1.652) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-53091
Antibody TypePrimary Antibodies
NCBI Gene AliasesMyHC-peri, DA2B3, MYHC-EMB, gtMHC-F, CPSFS1A, MYHSE1, CPSFS1B, Myhse, CPSKF1B, CPSKF1A, DA7, HEMHC, MyHC-emb, DA2B, DA2A, MyHC-pn, DA8, SMHCE, Myhs-e
CloneF1.652
Quantity200 µg/ml
Reactivitymouse, rat, human, rabbit
ApplicationsWB, IP, IF, IHC(P)
FormatPurified
Conjugate/TagUnconjugated
Hostmouse
ClonalityMonoclonal
NCBI Full Gene Namemyosin heavy chain 3, myosin, heavy polypeptide 3, skeletal muscle, embryonic, myosin heavy chain 8
IsotypeIgG1
TargetMYH3

Questions and Answers

Can this antibody be used for detecting myosin heavy chain 3 in muscle tissue samples?

Yes, the MYH3 (F1.652) Antibody has been utilized in immunohistochemistry to detect embryonic myosin heavy chain in muscle tissue sections, as demonstrated in studies involving mouse models of muscular dystrophy and muscle regeneration ().

What species can this antibody react with in myogenic studies?

The MYH3 antibody is reactive with multiple species, including humans, mice, rats, and rabbits, making it suitable for comparative studies across different animal models and human tissues ().

Has this antibody been cited in research related to muscle regeneration?

Yes, the MYH3 antibody has been cited in various studies focusing on muscle regeneration and differentiation, including its role in the expression of myosin heavy chain during muscle repair processes in mouse models (, ).

What is the recommended dilution for using this antibody in Western blot applications?

The MYH3 antibody is recommended to be used at a dilution of 1:500 for Western blot applications, as indicated in studies assessing myosin heavy chain expression in muscle samples ().

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) The therapeutic potential of Myoki, a novel peptide in muscle atrophy: mechanisms and applicationsFrontiers in PharmacologyFebruary 12, 2026Eun Mi Kim, Seon Soo Kim, Yong Geon Hyun, Su Yeon Lee, Yong Ji Chungincubated overnight with primary antibodies, anti-MyoD (Santa Cruz, sc-377460) and anti-MYH3 (Santa Cruz, sc-53091), followed by washing with PBS. The samples were then incubated for 1 h Alexa Fluor 488–conjugated goat

(2) TRPV1 manipulating polarization of M1/M2 macrophages to promote skeletal muscle regenerationSkeletal MuscleFebruary 3, 2026Jinfang Liu, Na Li, Shuwei Jing, Fangyu Wu, Guoshuai An, Liangliang Wang, Jian Li, Ximei Cao, Junhong Sunwith the primary antibody for TRPV1, CD86, CD206, mouse anti-MYH3 (1:200, Santa Cruz Biotechnology, sc-53091), rabbit anti-IL-6 polyclonal antibody (1:200, Proteintech, #21865-1-AP), and rabbit anti-Arginase-1

(3) Regenerative Index reveals declining muscle regeneration in paediatric patients with Duchenne muscular dystrophybioRxivJanuary 7, 2026Johnathan K. Smid, Charis A. McPherson, Jacob G. Monast, Shanti S. S. Rayagiri, Steven A. Moore, Michael A. RudnickiMouse IgG2a anti-human IgG mAb (Abcam, Cat# ab200699) and Mouse IgG1 anti-eMHC mAb (Santa Cruz, Cat# sc-53091) were prepared in PBS at a dilution of 1:200. In addition, to identify myofiber membranes Rabbit Anti-Laminin

(4) Compromised Regeneration, Damage to Blood Vessels and the Endomysium Underpin Permanent Muscle Damage Induced by Puff Adder (Bitis arietans) VenomToxins (Basel)October 6, 2025Sodiq Opeyemi Adeyemi, Nicholas John Richards, Ali Alqallaf, Husain Bin Haidar, Mustafa Jawad Jalil Al-Asady, Jarred Williams, José R. Almeida, Ketan Patel(Clone A14.1025 DSHB, USA cell supernatant) 1:200, Mouse anti-Myosin Heavy Chain 3, 1:200, (Santa Cruz, SC53091, USA), Rabbit anti-Laminin, 1:200, (Sigma Aldrich, L9393, UK), Rabbit anti-Dystrophin, 1:200, (Abcam

(5) Gallic Acid Improves Muscular Function Through Enhanced Myoblast Myogenesis in MiceFood Sci NutrJuly 18, 2025Sangsoo Lee, Dong-Yup Han, Kee K. Kimat room temperature, followed by overnight incubation with primary antibodies against Myh3 (1:500, SC‐53091, Santa Cruz) and GAPDH (1:2000, SC‐47724, Santa Cruz). After washing with PBST, immunoreactive proteins

(6) Immune aging impairs muscle regeneration via macrophage-derived anti-oxidant selenoprotein PEMBO RepJuly 18, 2025Dieu-Huong Hoang, Jessica Bouvière, Johanna Galvis, Pauline Moullé, Orane Mercier, Eugenia Migliavacca, Ananga Ghosh, Gaëtan Juban, Sophie Liot, Pascal Stuelsatz, et al.anti-PDGFRα (AF1062, RD systems), anti-CD31 (ab7388, Abcam), anti-F4/80 (ab6640, Abcam), anti-eMHC/MYH3 (sc-53091, Santa Cruz), anti-Pax7 (hybridoma from DSHB), anti-perilipin (ab3526, Abcam), revealed by secondary

(7) Forskolin treatment enhances muscle regeneration and shows therapeutic potential with limitations in Duchenne muscular dystrophySkelet MuscleMay 7, 2025Andreea Iuliana Cojocaru, Kaouthar Kefi, Jean-Daniel Masson, Laurent Tiret, Frederic Relaix, Valentina Tagliettiovernight at 4 °C with primary antibodies specific for regenerating myofibers (eMHC, MYH3 Antibody sc-53091, Santa Cruz), FAPs (PDGFRα, Tebubio, 250E-AB-22250), MuSCs (Pax7, DSHB), and macrophages (CD68, ab31630,

(8) RNF138 regulates skeletal muscle differentiation via the Wnt/β-catenin signaling pathwayTheranosticsMarch 18, 2025Wenhao Wang, Zhuohua Wang, Rourong Li, Weiyi Huang, Qiao Ling, Xiaoxiao Li, Zan Li, Manqi Cao, Zhihui Zhang, Qingrong Sun, et al.antibodies: anti-RNF138 (Cat# A10304, ABclonal, China) anti-MyHC (Cat# sc-376157, Santa Cruz, USA) anti-eMyHC (Cat# sc-53091, Santa Cruz, USA) anti-Myogenin (Cat# ab1835, Abcam, UK) anti-APC (Cat# ab40778, Abcam, UK) anti-β-catenin

(9) Enhanced expression of dystrophin, IGF-1, CD44 and MYH3 in plasma and skeletal muscles including diaphragm of mdx mice after oral administration of Neu REFIX beta 1,3-1,6 glucanScientific ReportsFebruary 28, 2025Senthilkumar Preethy, Shuji Sakamoto, Takuma Higuchi, Koji Ichiyama, Naoki Yamamoto, Nobunao Ikewaki, Masaru Iwasaki, Vidyasagar Devaprasad Dedeepiya, Subramaniam Srinivasan, Kadalraja Raghavan, et al.mounted with ProLong Gold antifade reagent (Invitrogen, USA). The following antibody was used: Myh3 (sc-53091, Santa Cruz Biotechnology, 1:100). Images were taken with all-in-one fluorescence microscope BZ-X800

(10) Neuromuscular electrical stimulation training induces myonuclear accretion and hypertrophy in mice without overt signs of muscle damage and regenerationSkelet MuscleFebruary 5, 2025Aurélie Fessard, Aliki Zavoriti, Natacha Boyer, Jules Guillemaud, Masoud Rahmati, Peggy Del Carmine, Christelle Gobet, Bénédicte Chazaud, Julien Gondinprimary antibodies were used: anti-MYH3 (i.e., to label myosin heavy chain embryonic; 1/200, mouse, sc-53091, Santa Cruz Biotech), anti-PCM1 (1/1000, rabbit, HPA023370, Sigma), anti-Laminin (1/200, rabbit, L9393,

(11) Tissue-resident skeletal muscle macrophages promote recovery from viral pneumonia-induced sarcopenia in normal agingbioRxivJanuary 14, 2025Constance E. Runyan, Lucy Luo, Lynn C. Welch, Ziyan Lu, Fei Chen, Maxwell J. Schleck, Radmila A. Nafikova, Rogan A. Grant, Raul Piseaux Aillon, Karolina J. Senkow, et al.F4/80-Biotin (1:200 Biolegend catalog 123106)) and MYH3 (1:400 dilution; Santa Cruz catalog number sc-53091) Overnight at 4°C in a humidified chamber. Secondary antibody incubation was performed at room temperature

(12) Progressive cardiomyopathy with intercalated disc disorganization in a rat model of Becker dystrophyEMBO reportsNovember 1, 2024Taglietti V, Kefi K, Mirciloglu B, Bastu S, Masson JD, Bronisz-Budzyńska I, Gouni V, Ferri C, Jorge A, Gentil C, et al.(Leica, B-DG-CE), anti-nNOS (Thermofisher, Catalog #61-7000), anti-eMHC (Santa Cruz Biotechnology, sc-53091), anti-laminin (Sigma-Aldrich, L9393), anti-Connexin-43 (Millipore, C6219), anti-N-cadherin (Sigma-Aldrich,

(13) Steamed Ginseng Berry Powder Ameliorates Skeletal Muscle Atrophy via Myogenic EffectsJournal of Microbiology and BiotechnologyJanuary 28, 2024Jungbum Kim, Hui-Ji Choi, Donghyuk Seo, Sang-Ah Lee, Jong Beom Heo, Dong Hyuk Baek, Wonhwa Lee, Gyu Yong Song(MAB1914P, Millipore, USA), mouse anti-AREG (sc-74501, Santa Cruz Biotechnology), mouse anti-e-MyHC (sc-53091, Santa Cruz Biotechnology), rabbit anti-laminin (ab11575, abcam), and mouse anti-MyoD (sc-377186, Santa Cruz Biotechnology

(14) AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretioniScienceDecember 15, 2023Kneppers A, Ben Larbi S, Theret M, Saugues A, Dabadie C, Gsaier L, Ferry A, Rhein P, Gondin J, Sakamoto K, et al.α7integrin-647,AbLab,Cat#67-0010-05; Clone R2F2 embryonic Myosin Heavy Chain (eMyHC),Santa Cruz,Cat#sc-53091 Laminin α1 (Laminin),Sigma-Aldrich,Cat#L9393 Pericentriolar Material 1 (PCM1),Sigma-Aldrich,Cat#HPA023370 Laminin

(15) Myopathologic trajectory in Duchenne muscular dystrophy (DMD) reveals lack of regeneration due to senescence in satellite cellsActa Neuropathologica CommunicationsOctober 19, 2023Nastasia Cardone, Valentina Taglietti, Serena Baratto, Kaouthar Kefi, Baptiste Periou, Ciryl Gitiaux, Christine Barnerias, Peggy Lafuste, France Leturcq Pharm, Juliette Nectoux Pharm, et al.myosin heavy chain (eMHC) as a marker of newly regenerated myofibers [34] (Santa Cruz Biotechnology, sc-53091), Laminin (Sigma-Aldrich, L9393) and CD45 (BD biosciences, 555,843). Alexa fluo secondary antibodies

(16) Pax7 haploinsufficiency impairs muscle stem cell function in Cre-recombinase mice and underscores the importance of appropriate controlsStem Cell Research & TherapyOctober 13, 2023Despoina Mademtzoglou, Perla Geara, Philippos Mourikis, Frederic Relaix1/200 Rabbit polyclonal anti-Laminin,Sigma L9393,IF 1/500 IgG1 mouse monoclonal eMyHC,Santa Cruz sc-53091,IF 1/200 Mouse monoclonal mCadherin,Nanotools MCAD-12G4,IF 1/100 "Goat anti-mouse IgG1, Alexa Fluor

(17) TGF-β1 stimulation and VDR-dependent activation modulate calcitriol action on skeletal muscle fibroblasts and Smad signalling-associated fibrogenesisSci RepAugust 23, 2023Ratchakrit Srikuea, Muthita Hirunsaimouse monoclonal anti-MyoD (G-1) (1:500, sc-377460), and mouse monoclonal anti-MYH3 (F1.652) (1:200, sc-53091) (Santa Cruz Biotechnology, CA, USA); rabbit monoclonal anti-vimentin (D21H3) XP® (1:400, 5741) and

(18) Indian Ornamental Tarantula (Poecilotheria regalis) Venom Affects Myoblast Function and Causes Skeletal Muscle DamageCellsAugust 15, 2023Richards NJ, Alqallaf A, Mitchell RD, Parnell A, Haidar HB, Almeida JR, Williams J, Vijayakumar P, Balogun A, Matsakas A, et al.The primary antibodies used in this study were mouse anti-Myosin heavy chain 3, 1:200, (Santa Cruz, SC53091, USA), Rabbit anti-Dystrophin, 1:200, (Abcam, 15277, UK), Rabbit anti-Laminin, 1:200, (Sigma Aldrich,

(19) Slo1 deficiency impaired skeletal muscle regeneration and slow-twitch fibre formationJournal of cachexia, sarcopenia and muscleAugust 1, 2023Xia C, Wang Y, Jiang T, Hu Y, Chen Y, Ma X, Zhang X, Gao Y.MP Imaging System (Bio‐Rad). Primary antibodies and dilutions used were as follows: MYH3 (cat. no. sc‐53091), Pax7 (cat. no. sc‐81648), MyoG (cat. no. sc‐12732) (1:200, Santa Cruz Biotechnology); Slo1 (1:1000,

(20) Endurance Exercise-Induced Fgf21 Promotes Skeletal Muscle Fiber Conversion through TGF-β1 and p38 MAPK Signaling PathwayInternational journal of molecular sciencesJuly 13, 2023Luo X, Zhang H, Cao X, Yang D, Yan Y, Lu J, Wang X, Wang H.

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MYH3 (F1.652) Antibody

MYH3 (F1.652) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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MYH3 (F1.652) Antibody from Santa Cruz Biotechnology, Inc.

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