Fig 1: Heterogeneous nuclear ribonucleoprotein K (hnRNPK) interacts with peroxiredoxin 5 (Prdx5) in osteoblasts.(A) To determine co-localization, osteoblasts were stained with antibodies against Prdx5 and hnRNPK, and images were acquired via confocal microscopy (scale bar, 20 µm). The upper images were magnified as depicted by the dotted box in the lower images. (B) Immunoprecipitation (IP) was performed using HEK293T cells expressing various combinations of HA-tagged Prdx5 and flag-tagged hnRNPK. (C) Osteoblasts were differentiated from the precursors derived from wild-type (WT) and Prdx5Ko mice via bone morphogenic protein 2 (BMP2) treatment for 7 days. hnRNPK localization was analyzed via confocal microscopy (scale bar, 20 µm). (D) hnRNPK levels were determined in the cytoplasmic and nuclear fractions of WT and Prdx5Ko cells. Osteoblasts were harvested on day 7 after BMP2 stimulation. (E) Osteocalcin luciferase assay was performed using MC3T3-E1 cells differentially expressing Prdx5 and BMP2 stimulation. Data are presented as mean ± SD. **p <0.01 via an unpaired two-tailed Student’s t-test. Figure 6—source data 1.Full blots for Figure 6B and D. Figure 6—source data 2.Original blots for Figure 6B and D.